Ptch2 (NM_008958) Mouse Untagged Clone

CAT#: MC223858

Ptch2 (untagged) - Mouse patched homolog 2 (Ptch2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptch2
• Synonyms: ptc; ptc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223858 representing NM_008958
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCGGCCACTGTCCCTTGGAGAGCTACCTCCCAGCTACACACCTCCAGCTCGGTCCTCCGCACCTC
  ATATCCTAGCTGGGAGCCTGCAGGCTCCTCTCTGGCTTCGAGCTTACTTCCAAGGTCTACTCTTCTCCCT
  GGGGTGCAGGATCCAGAAACACTGCGGCAAAGTGCTCTTCCTGGGACTGGTGGCCTTTGGGGCTCTGGCA
  CTGGGTCTCCGAGTGGCTGTAATTGAGACAGACCTAGAACAGCTCTGGGTAGAAGTGGGCAGCAGGGTGA
  GCCAGGAGCTGCACTACACCAAGGAGAAGCTGGGGGAAGAGGCTGCGTACACCTCCCAGATGTTGATTCA
  GACTGCGCACCAGGAAGGGGGAAACGTCCTCACCCCCGAGGCACTTGACTTGCACCTCCAGGCGGCGCTC
  ACTGCCAGTAAAGTCCAAGTATCACTCTATGGGAAATCCTGGGATTTGAACAAGATCTGCTACAAATCAG
  GGGTTCCCCTTATTGAAAATGGGATGATCGAGCGGATGATTGAGAAGCTGTTTCCCTGTGTGATCCTCAC
  CCCGCTTGACTGCTTCTGGGAAGGAGCCAAACTCCAAGGGGGCTCTGCCTACTTGCCGGGCCGCCCTGAT
  ATCCAGTGGACCAACCTGGACCCTCAGCAGCTGCTCGAGGAGCTGGGCCCCTTTGCCTCTCTGGAGGGCT
  TCCGGGAACTGCTAGATAAGGCACAGGTGGGCCAGGCCTATGTGGGGCGACCCTGTCTGGACCCTGATGA
  CCCCCACTGTCCACCTAGTGCTCCCAACCGGCACAGCAGGCAGGCTCCCAATGTGGCTCAGGAGCTGAGT
  GGGGGCTGCCATGGCTTCTCCCACAAGTTCATGCACTGGCAGGAGGAACTGCTACTAGGGGGCACAGCCA
  GAGATCTCCAAGGACAGCTGCTGAGGGCAGAGGCCTTGCAGAGCACCTTCCTGCTCATGAGTCCCCGTCA
  GCTGTACGAGCACTTCCGGGGCGACTACCAGACACATGACATCGGCTGGAGCGAGGAGCAGGCCAGCATG
  GTGCTGCAGGCCTGGCAGAGGCGCTTTGTGCAGCTAGCCCAGGAGGCTCTGCCGGCCAACGCGTCCCAGC
  AGATCCATGCCTTCTCCTCCACCACCCTGGATGACATCCTGCGCGCGTTCTCTGAAGTCAGCACCACCCG
  TGTGGTAGGAGGCTATCTGCTTATGCTGGCCTATGCCTGCGTAACAATGCTGCGGTGGGACTGCGCCCAG
  TCCCAGGGTGCCGTAGGTCTTGCTGGGGTGTTGCTGGTGGCCCTGGCGGTGGCCTCAGGTCTTGGGCTTT
  GTGCCCTACTTGGCATCACTTTCAATGCTGCCACTACACAGGTGCTGCCCTTCTTGGCTCTGGGCATTGG
  CGTGGATGACATCTTCCTGCTGGCGCATGCCTTCACAAAAGCCCCACCTGACACCCCTCTCCCAGAGCGT
  ATGGGTGAGTGTTTGAGGAGCACGGGCACCAGCGTGGCACTCACATCCGTCAACAACATGGTCGCCTTTT
  TCATGGCTGCCCTGGTTCCCATTCCTGCGCTGCGGGCCTTCTCCCTGCAGGCAGCCATAGTGGTCGGCTG
  CAACTTTGCAGCGGTAATGCTCGTCTTCCCTGCCATCCTCAGCCTCGACCTGCGCCGGCGCCACCGCCAA
  CGCCTGGACGTTCTCTGCTGCTTTTCTAGCCCCTGCTCCGCTCAGGTCATTCAGATGCTGCCCCAGGAGC
  TAGGGGACAGAGCAGTACCAGTGGGCATTGCCCACCTGACTGCCACCGTGCAAGCCTTCACCCACTGCGA
  AGCCAGCAGCCAGCATGTAGTCACCATTTTGCCTCCTCAAGCCCACCTGCTGTCTCCAGCTTCTGACCCA
  CTGGGCTCCGAACTCTATAGCCCTGGAGGGTCTACACGGGACCTTCTCAGTCAGGAGGAGGGGACAGGGC
  CACAGGCGGCCTGCAGGCCCCTGCTCTGTGCCCACTGGACTCTCGCCCATTTTGCCCGCTATCAGTTTGC
  ACCTTTACTGCTCCAGACACGAGCCAAGGCCCTGGTGTTGCTGTTCTTTGGGGCTCTTTTGGGCCTGAGC
  CTCTATGGAGCCACCTTGGTACAAGATGGGCTGGCCCTGACAGATGTGGTCCCTAGGGGCACCAAGGAAC
  ATGCTTTCCTGAGCGCCCAGCTCAGGTACTTCTCCCTGTACGAGGTGGCTCTAGTGACACAGGGTGGCTT
  TGACTACGCCCACTCCCAACGCGCCCTCTTTGATCTGCACCAGCGCTTCAGCTCCCTCAAGGCTGTGCTG
  CCCCCACCTGCCACCCAGGCACCCCGCACCTGGCTTCACTACTACCGCAGCTGGCTACAGGGTATCCAGG
  CTGCATTTGACCAAGACTGGGCTTCTGGGCGCATCACCTGCCACTCTTACCGCAACGGCTCAGAAGATGG
  CGCCCTGGCCTACAAGCTGCTCATCCAAACCGGGAATGCCCAGGAGCCTCTGGATTTCAGCCAGCTGACC
  ACAAGGAAACTGGTGGACAAGGAGGGACTCATTCCCCCAGAGCTCTTCTACATGGGGCTAACCGTGTGGG
  TGAGCAGCGACCCCCTGGGCCTCGCAGCCTCTCAGGCCAACTTCTACCCCCCACCTCCAGAGTGGCTTCA
  CGACAAATATGATACCACCGGGGAGAACCTTCGCATCCCGGCAGCCCAGCCCTTGGAGTTTGCCCAGTTC
  CCCTTCCTGCTGCATGGACTCCAGAAGACTGCAGACTTTGTAGAAGCCATCGAAGGGGCCAGGGCGGCAT
  GCACAGAGGCAGGCCAGGCAGGGGTGCATGCCTACCCCAGTGGCTCCCCCTTCCTCTTCTGGGAGCAGTA
  TCTGGGTCTTCGGCGCTGCTTCCTGCTGGCAGTCTGCATCTTGCTGGTGTGCACCTTCCTCGTCTGTGCC
  CTGCTTCTACTCAGCCCGTGGACAGCTGGCCTCATAGTGCTGGTCCTGGCAATGATGACTGTGGAGCTCT
  TTGGTATCATGGGATTCCTGGGCATCAAACTGAGTGCCATCCCCGTGGTAATCCTCGTGGCCTCTATAGG
  CATTGGTGTTGAATTCACAGTTCACGTGGCTCTGGGCTTCCTGACCAGCCATGGTAGCCGGAACCTGCGG
  GCTGCTAGCGCTCTGGAACAGACCTTTGCCCCTGTGACTGATGGAGCTGTCTCCACCTTGCTGGGTCTGC
  TCATGCTTGCTGGTTCTAACTTTGACTTCATCATAAGGTATTTCTTTGTGGTGCTGACGGTGCTGACACT
  CTTGGGCCTGCTCCATGGACTCCTGCTGCTGCCTGTGCTGCTCTCTATCCTAGGCCCCCCGCCACAGGTG
  GTGCAGGTATACAAGGAGAGTCCACAGACCCTAAACTCCGCTGCTCCACAGAGAGGTGGGCTCAGGTGGG
  ACAGGCCCCCCACCCTGCCCCAGAGTTTTGCCAGAGTGACTACCTCCATGACTGTGGCCCTCCACCCACC
  ACCTCTGCCTGGAGCATACGTCCACCCAGCCTCCGAGGAGCCCACATAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_008958
• Insert Size: 3549 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008958.3, NP_032984.1
• RefSeq Size: 5119 bp
• RefSeq ORF: 3549 bp
• Locus ID: 19207
• UniProt ID: O35595
• Cytogenetics: 4 53.41 cM
• Gene Summary: This gene encodes a member of the patched family of transmembrane receptor proteins. The encoded protein may be a functional receptor for the morphogen sonic hedgehog (Shh) and is reportedly involved in limb and skin development. Homozygous mutant mice for this gene exhibit hair loss and epidermal hyperplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).