Wdr35 (NM_172470) Mouse Untagged Clone

CAT#: MC223848

Wdr35 (untagged) - Mouse WD repeat domain 35 (Wdr35), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wdr35
• Synonyms: 4930459M12Rik; 4931430C06; mKIAA1336
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223848 representing NM_172470
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTTCTACCTGAGCAAGAAAATTGCTGTTCCCAATAATGTGAAGCTGAAATGTATATCCTGGAACA
  AGGACCAAGGGTTCATAGCATGTGGCGGTGAGGATGGATTACTGAAAGTTTTAAGACTCGAGACACAGAC
  AGATGACTCAAAGCTGAGGGGTCTAGCCGCCCCTAGTAACCTTTCCATGAACCAAAATCTTGAAGGTCAT
  AGTGGTGCTGTTCAAGTTGTAACGTGGAATGAACAGTATCAGAAGTTGACTACCAGTGATCAAAATGGGC
  TTATCATTGTCTGGATGCTGTATAAAGGCTCTTGGTATGAGGAGATGATCAACAATCGCAACAAGTCTGT
  GGTTCGAAGCATGAGCTGGAATGCGGACGGACAGAAGATCTGCATTGTGTACGAAGATGGAGCTGTGATC
  GTCGGCTCAGTAGACGGGAATCGGATTTGGGGGAAAGACCTGAAGGGAATTCAGCTGTGCCATGTGACCT
  GGTCTGCAGATAGTAAAATCCTACTTTTTGGAATGGCAAATGGAGAAATACACATTTATGATAATCAAGG
  AAATTTTATAATGAAAATGAAGCTGAATTGCTTGGTGAACGTCACTGGAGCGATCAGTATCGCTGGGATT
  CACTGGTACCATGGCACCGAAGGCTACGTGGAGCCCGATTGCCCTTGCCTGGCAATTTGCTTTGACAATG
  GGAGATGCCAAATAATGCGGCATGAGAATGACCAAAACCCGGTTTTGATCGACACGGGCATGTATGTGGT
  AGGCATCCAGTGGAACCACATCGGCAGTGTGTTAGCTGTGGCCGGCTCCCAGAAGGTAGTCACTCAGGAC
  AAGGACATCAACATCGTGCAGTTCTACACACCGTTCGGTGAGCATCTGGGTACTTTGAAAGTTCCAGGAA
  AGCAGATGTGTTCGCTGTCTTGGGAAGGAGGCGGACTGAAGATTGCTCTAGCTGTTGACTCCTTTATATA
  TTTTGCAAATATTCGACCTGATTATAAGTGGGGCTACTGTTCCAACACAGTGGTTTATGCGTACACCAGA
  CCCGATCGTCCCGAATACTGTGTTGTGTTCTGGGACACAAAAAACAGTGAGAAATACGTTAAATATGTGA
  AGAGCCTCATTTCTATCACCACGTGTGGAGATTTCTGCATTTTGGCTACAAAAGCTGATGAGAATCACCC
  TCAGGAGGAGAATGAAATGGAGACATTTGGTGCAACGTTTGTGCTTGTCCTTTGCAATTCTATTGGCACA
  CCCTTGGACCCTAAATACATTGATCTTGTACCATTATTTGTTGCAATGACCAAAACCCATGTGATAGCAG
  CTTCAAAAGAAGCATTTTATACCTGGCAATATCGTGTGGCAAAGAAGCTCACAGCACTGGAAATTAATCA
  GATCACACGGTCTCGAAAAGAAGGAAGAGAAAGAATTTATCACGTTGATGATGTCCCTTCCGGATCTGTG
  GATGGGGTGTTTGATTACAGTAAAGCCATTCAAGGTACAAGGGATCCAATTTGTGCCATTACTGCATCTG
  ATAAGACATTGATTGTGGGGCGTGAATCTGGCGTCATTCAGAGGTACAGCTTTCCTAATGTTGCTCTGAT
  TCAAAAGTATTCCCTGGATTGCCGTGCCTGCCAGTTATCTCTGAACTGCAACTCCAGTCGTCTTGCTATC
  ATCGACATCGCTGGAGTCTTGACTTTCTTTGACTTGGACACTCGGGTGACCGACAGTACAGGGCAGCAGG
  TCGTTGGCGAGTTGCTAAAACTGGAGCGCAAAGACGTCTGGGATATGAAGTGGGCCAAGGACAATCCTGA
  TTTGTTTGCAATGATGGAGAAGACGAGAATGTATGTTTTCAGAAACTTGGATCCTGAGGAACCCATTCAG
  ACCTCTGGATATATTTGCAACTTTGAGGATTTGGAAATTAAGTCTGTTCTCTTGGATGAGATACTGAAGG
  ACCCAGAGCACCCTAGCAAGGATTACATAATGAACTTTGAGATCCGGTCCCTGCGAGACAGCCGAGCATT
  GATTGAGAAAGTTGGAATTGAAGATGCGTCTCAGTTCATAGAGGACAACCCACACCCCCGACTTTGGCGC
  CTGCTGGCTGAAGCTGCTCTCCAGAAGCTGGACCTGTACACCGCACAGCAAGCGTTTGTGCGCTGCAAGG
  ATTACCAAGGCATCAAGTTTGTGAAGCTCCTGGGCAATCTGCAGAGCGAGTCAATGAAGCAGGCTGAAGT
  TATTGCCTACTTTGGCAGGTTCGAAGATGCCGAAAGGATGTATCAAGATATGGACAGACGGGACCTCGCT
  ATTGGTCTCAGAATGAAATTGGGGGACTGGTTCAGAGTACTGCAGCTCCTGAAAACTGGATCTGGTGATG
  CAGACGACAGTCTCCTTGAACAAGCTAACAATGCCATTGGAGAGTACTTTGCTGACCGACAGAAGTGGCA
  GAATGCTGTGCAATATTATGTAAAAGGCAGGAACCAGGAGCGCCTGGCCGAATGCTATTATATGCTAGAA
  GATTATGAGGGGTTAGAGACTCTTGCCAATTCACTTCCAGAAAACCATAAGTTGCTTCCAGAAATAGCCC
  AGATGTTTGTGAGAGTTGGAATGTGTGAGCAAGCTGTGAGCGCATTCTTGAAGTGTAACCAACCAAAGGC
  AGCGGTGGACACCTGTGTACACCTGAACCAATGGAACAAAGCCGTCGAACTGGCTAAAAGTCATAGTATG
  AAGGAGATTGGATCTCTGTTAGCTAGGTACGCGTCCCATTTACTGGAGAAGAACAAGACTCTCGATGCCA
  TTGAACTCTATAGGAAAGCCAGCTACTTTTTTGATGCAGCTAAACTGATGTATAAGATTGCAGATGAAGA
  GGCGAAGAAAAGGACCAAGCCCCTGCGTGTGAAGAAACTCTACGTGCTGTCGGCTCTGCTCATCGAGCAG
  TATCACGAGCAGATGAAAAACGCCCAGCGCGGCAAAGTTAAAGGCAAGAACTCAGAGGCCACTTCTGCTT
  TGGCTGGCTTGCTAGAAGAGGAGGTTCTCTCTACAACTAGTCGGTTCACAGATAACGCCTGGAGAGGAGC
  GGAGGCCTACCATTTCTTTATACTCGCGCAGCGGCAGCTCTACGAGGGCTACGTTGACACTGCATTGAAG
  ACAGCTCTTCACCTGAGAGACTATGAAGACATCATTCCCTCAGTTGAGATCTACTCTCTGTTAGCACTCT
  GTGCGTGTGCAAGCAGAGCTTTTGGTACCTGTTCAAAAGCTTTTATTAAACTGGAATCTTTAGAGACGCT
  CAGTGCAGAGCAGAAGCAGCAATATGAAGACCTGGCTCTGGAAATCTTCACCAAACACACGCCAAAAGAC
  AACAGGAAGTCTGAATTAAACAGCCTTCTTGAAGGCGGGGAAGGCAAACTGCCGACCTGTATCGCCACTG
  GCAGCCCAATCATCGAGTATCAGTTCTGGGTGTGCAAAGTCTGCAAGCACTACGTCCTCGCCCAGGAGAT
  CAGCAACTACAACTTTTGCCCTTTATGCCACAGCTCGGTAGAATAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_172470
• Insert Size: 3546 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172470.3, NP_766058.3
• RefSeq Size: 4409 bp
• RefSeq ORF: 3546 bp
• Locus ID: 74682
• UniProt ID: Q8BND3
• Cytogenetics: 12 A1.1
• Gene Summary: As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in ciliogenesis and ciliary protein trafficking (PubMed:21473986). May promote CASP3 activation and TNF-stimulated apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).