Rtel1 (NM_001166666) Mouse Untagged Clone

CAT#: MC223841

Rtel1 (untagged) - Mouse regulator of telomere elongation helicase 1 (Rtel1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rtel1
• Synonyms: AI451565; AW540478; Rtel
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223841 representing NM_001166666
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGGGTAGTCCTGAATGGTGTGACAGTGGATTTTCCTTTCCAGCCCTACCCATGCCAACAGGAAT
  ATATGACCAAGGTGCTAGAATGTCTCCAGAAGAAAGTGAATGGCATCCTGGAGAGCCCCACAGGCACTGG
  GAAGACGCTGTGCCTCCTCTGTTCCACCTTGGCCTGGCAACAACATCTCCGAGATGCAGTTTCTTCCCTA
  AAGATTGCTGAGAGAGTTCAAGGGGAACTCTTTGCCAGTCGGACCTTGTCATCCTGGGGGAGTGCTGCTG
  CCGCCAGCGGAGACTCAATAGAGTGCTACACAGATATCCCAAAGATCATCTATGCTTCTAGAACGCACTC
  CCAGCTAACTCAGGTCATCCGTGAGCTTCGGAATACCGCCTACCGGCCCAAGGTATGTGTGCTGGGCTCC
  CGGGAGCAGCTGTGTATTCATCCTGAAGTGAAGAAGCAGGAGAGTAATCACATGCAGATCAGTTTGTGCC
  GCAAGAAGGTAGCAAGTCGCTCCTGTCATTTCTACAACAATGTGGAAGCGAAATTCCTGGAGCAAGATTT
  GGCTACCCCCATCCTGGATATTGAGGACCTTGTCAAGAATGGAAGCAAACAAAAAATGTGCCCATACTAC
  CTTTCTCGAAACATGAAACAGCAAGCGGACATCATCTTTATGCCATACAATTACCTGTTGGATGCTAAGA
  GTCGTAAGGCGCACAGCATTGACCTGAAGGGAACAGTTGTGATCTTTGATGAAGCTCACAATGTGGAGAA
  GATATGTGAGGAGTCAGCCTCCTTTGACTTGACTCCCCGTGATGTGGCTTCAGGACTGGAGATTATCAAC
  CAGGTTTTGGAGGAACAAGCCCGGGTGACTCAGCAGGGTGAACTCCAACAGGAGTTCATTGTAGACACGT
  CCAGCTCAGGACTCAACATGGAGCTGGAAGACATCGCAAAGCTAAAGATGATCCTGCTTCGCCTGGAGGA
  GGCTATTGATGCCGTTCAGCTGCCTGGGGATGACAGAGGCGTCACCAAACCTGGAAGCTATATCTTCGAG
  CTGTTTGCTGAAGCTCAAATAACATTTCAAACCAAAGGCTGCATTTTGGAATCACTGGACCAGATAATCC
  AGCACCTGGCAGGCCGTACTGGTGTGTTCACCAACACGGCTGGGTTGCAGAAGCTTATGGACATTATCCA
  GATTGTGTTCAGCGTGGACCCTCCGGAAGGCAGCCCTGGTTCTCTGGTGGGGCTGGGTATCTCACATTCC
  TATAAGGTACACATACACCCTGAAACCAGCCACCGGAGAGCAGCTAAGCGGTCAGATGCCTGGAGTACCA
  CTGCATCCAGGAAACAAGGGAAGGTGCTGAGCTATTGGTGCTTCAGTCCCAGCCAGAGTATGCGGGAACT
  GGTCTGCCAAGGAGTTCGTACCCTTATCCTCACCAGCGGTACCCTGGCTCCACTGTCTTCCTTTGCTCTG
  GAGATGCAGATTCCATTCCCAGTCTGTCTGGAGAATCCACACATCATTGACAAGAACCAGCTCTGGGTGG
  GGATTGTCCCCAGAGGCCCTGATGGTGTTCAGCTAAGCTCTGCCTATGACAAAAGGTTTTCTGAAGAGTG
  TTTATCTTCCCTGGGGAAGGCTCTGAGCAACATTGCTCGCGTGGTGCCCCATGGGCTTCTGGTCTTCTTC
  CCTTCCTACCCTGTCATGGAGAAAAGCCTGGAGTTCTGGCAGGTACAAGGATTGGCCAGGAAGGTAGAGG
  CACTAAAGCCTCTGTTCGTGGAACCCAGGAACAAAGGCAGCTTCTCAGAGGTCATTGATGCCTACTACCA
  ACAAGTTGCCTCCCCTGCGTCTAATGGGGCCACCTTCTTAGCAGTGTGTCGGGGAAAGGCCAGCGAAGGG
  CTGGACTTCTCAGACATGAATGGTCGTGGTGTGATTGTCACGGGCCTCCCATATCCTCCACGCATGGATC
  CCCGTGTTGTCCTCAAGATGCAGTTCTTGGATGAGATGAGAGGCCGGAGTGGGGTTGGAGGCCAGTGCCT
  CTCTGGGCAGGAATGGTACCAGCAGCAAGCATCCAGGGCTGTGAACCAGGCTATTGGGAGGGTTATTCGA
  CACCGCCATGACTATGGGGCCATCTTCCTGTGTGACCACAGGTTCGCCTATGCTGATGCCAGGGCCCAGC
  TGCCCTCCTGGGTGCGCCCCTACCTTAAGGTGTATGACAACTTTGGCCATGTCATCCGAGATGTGGCCCA
  GTTCTTCCGTGTTGCTCAGAAAACTATGCCTTTGCCAGTTCCTCAGGCTGTGACCTCAAGTGTGAGTGAG
  GGAGAAATTGCTCTCAAGGATGCTACATTGTCCAGCTACTCCCTCTCTACCAGGAAAGCCATGAGTTTGG
  ATGTGCATGTGCCCAGCCTGAGGCAGAAGCCCATAGGATTACCAGCTGCTGGAGACTCTGAGAGCAGCCT
  GTGTGGGGAGTATGAGCAGCAGACATTTTCTGCCCAGCAGAGACCTATGGGACTGCTAGCTGCCTTAGAA
  TACAACGAGCAGAAGGCTGGGGCATCTGAGGAGCAGGCACTGGGCTCCTCCACCCCATCTCTCCGGTGCG
  AGAAGAGGCTGTCTACTGAGCAAAAAGGAGGAAGGAAGAAAGTCAGGCTGGTCAACCATCCGGAGGAACC
  AATGGCTGGCACACAGGCAGGCAGAGCCAAGATGTTCATGGTGGCTGTGAAGCAAGCACTGAGCCAAGCT
  AACTTTGACACCTTTACCCAGGCCCTGCAGCACTATAAGAGTTCTGATGACTTTGAAGCCCTAGTGGCCT
  CTCTCACCTGTCTCTTTGCTGAAGACCCCAAGAAACACACCCTGCTTAAAGGTTTCTACCAGTTTGTTCG
  ACCCCACCACAAGCAGCAGTTTGAGGACATCTGCTTCCAGCTAACAGGCCAACGATGTGGCTACCAGCCA
  GCTCATTTTTCCAAACCAGGACACACCAGCCATTGTACAAAAGTGGGATGTGCAGTAGAAAAACCTGGCC
  AGCCTGCTGTGAGTGACTACCTATCTGATGTCCACAAGGCTCTGGGATCTGCAAGCTGCAACCAGCTTAC
  GGCAGCTCTGAGGGCATACAAACAGGATGATGACCTGGACAAGGTGGTGGCTGTGGTGGCAGCACTGACC
  ACTGCAAAACCTGAACACTTACCCTTGCTACAGAGATTTGGCATGTTTGTGCGTCGGCATCATAAGCCTC
  AGTTCCTACAGACCTGTGCAGACCTAATGGGCCTGCCTACCACAGGCAAGGACTTGGAGCTCGAAGGTCC
  CAGAGATGAGAGCCCAACTGTGCCTCCTGAGCTTACCCATGAGGACCTGAAACCAGGGCCCTCGATGTCC
  AAGAAACCTGAGAAGACCCAGAGTAAGATCTCATCCTTCTTTAGACAGAGGCCAGATGAGAGTGTGAGGT
  CTGATGATACCACCCCAAAGCCCATGCAACTTCCTCCTAGACTACCCCATGAGCTTATGAAGCCTCATCG
  GAGTAAGCAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166666
• Insert Size: 3513 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166666.1, NP_001160138.1
• RefSeq Size: 4334 bp
• RefSeq ORF: 3513 bp
• Locus ID: 269400
• UniProt ID: Q0VGM9
• Cytogenetics: 2 H4
• Gene Summary: ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (3) lacks an internal segment, as compared to isoform 1.