Diaph3 (NM_019670) Mouse Untagged Clone

CAT#: MC223829

Diap3 (untagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Diaph3
• Synonyms: 4930417P13Rik; Dia2; Diap3; Drf3; mDia2; mKIAA4117; p134MDia2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223829 representing NM_019670
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGCACCGGGCGCGCGCTCTCGGCCGGGACAGCAAGTCGTCGCGGAGGAAGGGCTTGCAGTCCG
  CGCCGCCCGCTGGCCCCTACGAGCCCGGGGAGAAGCGACCCAAGTTGCATTTAAATATTAGAACACTGAC
  AGATGATATGCTGGACAAATTTGCCAGTATAAGAATTCCAGGGAGCAAGAAAGAGAGACCTCCCCTTCCC
  CACCTGAAGACTGTGTCTGGGATCAGTGACAGCTCATCACTGTCCTCAGAGACAATGGAAAACAACCCAA
  AGGCGCTGCCAGAGAGTGAAGTCTTGAAGCTTTTTGAGAAGATGATGGAAGATATGAATTTAAATGAAGA
  TAAAAAGGCACCATTGCGGGAAAAAGACTTCGGTATCAAAAAAGAAATGGTGATGCAGTACATTAATACT
  GCTTCTAAGACAGGAAGTCTTAGAAGTAGCCGACAGATCTCACCTCAGGAATTTCTCCATGAGCTGAAAA
  TGGGTTACACAGACGAGAGACTTTTCACGTATCTGGAGTCACTCCGAGTATCATTGACCAGTCATCCTGT
  GAGTTGGGTGCAAAGCTTTGGACACGAGGGACTCGGATTATTGCTGGACATTTTGGAAAAACTAATTAAT
  GGGCAAATCCAAGAAAAAGTTGTGAAGAAGACTCAGCACAAAGTCATCCAGTGTCTGAGAGCCCTGATGA
  ACACACAGTATGGCTTAGAAAGGATTATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGA
  TCCCAGGCAGCCCGCTATGATGGCAGACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAA
  AGCATCCTTGAAGAAGTGTTAGAAGCCTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTT
  CCATTGTGGAAGGCCTCCGGCATAACTCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCT
  CGTTACATCTCCTGATGATTTGGACTTCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAA
  GAGATATTGCCAAACTTAAAGGGCATTAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGC
  ACAAAGAAGAAGATTTGAGTGAGTTTTTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATC
  TGATGTTTACAGCATGTTATGGGACACAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTT
  CAGCATCTCCTGCTCATTCGCAATGATCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTG
  TGTCACAGATTGTATTACATAGAGATGGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGA
  CTTAAGTCAGTTTGTAGATGTTTGCATAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAA
  CATTGCAAGAAATTTGAAAAAGAGTGTACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGG
  CAAAGATTAATGAGCTTCAAGCAGAGTTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTAC
  AAAAATTCCTTTGCAACCTTCAGTAGAAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCA
  CTCAGTGGAGGAGTGCCGCCTCCCCCACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAA
  TGCCATTTGGTGGCCCTGTACCACCACCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATT
  AAACCTGCCATTTGGTTTGAAACCAAAGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGG
  TTAAAGATCGGACCAAATGAAATGTCTGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAA
  ATAGGGATTTGCTTTGTAAACTTGAGAACACTTTTTGTTGCCAAGAAAAAGAGAAAAGGAATACAAATGA
  CTTTGATGAGAAGAAAGTTATTAAGAAGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAG
  AACCTTTCAATCTTCCTGAGCTCCTTCCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGG
  ATGAAACACAGTTGTCAGAGTCCATGATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAA
  GTCATTGTCCCAGTTTAGAAGTGACTATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGC
  AATGTGAAGAGACTCCGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACA
  ACATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAA
  GTTGCTGGAACTTGTGTTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGA
  TTTGACCTTAGCTCTCTCTGTAAACTGAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATT
  TCCTGGTAGATGTATGTGAAGAAAAGCATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGA
  CAAAGCTAGCAGAGTCTCTGTAGAAATGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAG
  CTTGAGAAGAATTTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCA
  GCTTCGTTATCAGTGCGAACGAGCAGTATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTA
  CCAGAGTATAATGGGCTACTATGCTGTCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTG
  AACAACTTCAGAACTTCATTTATGCTAGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGG
  AGAAACGTGCCAGGATAGCGAAAGAGCGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCG
  CTTACTAGAAATGAAAACTGAGGGAGATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCA
  GGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGA
  TGTCTCAGAGGCCTGTTCTCAAAGTTTGTAACCATGAAAATCAGAAAATGCAGTTGACAGAAGGGTCACG
  TCCACACCACAGTATCAATTGCAACTCCACCAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGAC
  ACTCATGCGTCTACAGGGAGGATCAAGGCAGTTGAGAAGGAAGCCTGTAATGCAGAAAGCAACAAAAAAA
  AGGAAATGGAACTTCTTGGCTCTGTTGCTAAAAGCGAATCAGTTCCTGAAGTTGAAGCCCTGCTGGCAAG
  ATTACGAGCTTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019670
• Insert Size: 3516 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019670.1, NP_062644.1
• RefSeq Size: 3516 bp
• RefSeq ORF: 3516 bp
• Locus ID: 56419
• UniProt ID: Q9Z207
• Cytogenetics: 14 E1
• Gene Summary: Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).[UniProtKB/Swiss-Prot Function]