Ctr9 (NM_009431) Mouse Untagged Clone

CAT#: MC223825

Ctr9 (untagged) - Mouse Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Ctr9), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctr9
• Synonyms: AA409336; mKIAA0155; Sh2bp1; Tsbp; Tsp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223825 representing NM_009431
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGGGGCTCCATCGAGATTCCCCTCCGGGATACTGACGAGGTCATTGAACTTGACTTCGATCAGT
  TACCGGAGGGAGACGAAGTCATCAGCATTCTGAAGCAGGAGCACACACAACTGCACATATGGATTGCCTT
  GGCGCTGGAATACTACAAGCAAGGGAAAACAGAAGAGTTTGTGAAGCTGCTGGAGGCAGCACGGATAGAT
  GGCAATTTGGACTATCGAGATCACGAGAAAGACCAGATGACTTGCTTGGATACATTGGCAGCCTACTATG
  TACAACAGGCTCGGAAAGAAAAGAACAAGGACAATAAGAAGGACCTTATTACGCAGGCAACTTTGTTATA
  TACAATGGCTGATAAAATTATTATGTATGATCAGAACCATTTGTTGGGAAGAGCCTGCTTCTGTCTGCTC
  GAGGGCGACAAAATGGACCAAGCTGACGCACAGTTTCACTTTGTACTTAACCAGTCTCCAAACAATATTC
  CAGCCCTTCTTGGGAAAGCTTGTATTTCATTCAACAAGAAGGATTACAGAGGAGCTCTGGCTTACTATAA
  AAAAGCATTGCGCACTAACCCGGGATGTCCAGCTGAAGTTCGTTTAGGAATGGGCCATTGCTTTGTCAAA
  CTTAACAAACTGGAAAAAGCTCGACTGGCATTCAGCAGAGCCCTGGAACTCAACTCGAAGTGTGTGGGAG
  CACTGGTTGGACTGGCTGTTCTAGAACTCAACAATAAAGAGGCTGATTCCATCAAAAATGGCGTGCAGCT
  TCTTTCCAGAGCCTATACTATTGATCCTAGCAACCCGATGGTACTGAACCATTTGGCAAACCACTTTTTC
  TTCAAGAAGGATTATAGTAAAGTGCAGCACCTGGCTCTCCACGCATTCCACAACACCGAGGTGGAGGCTA
  TGCAAGCAGAAAGCTGTTACCAGCTGGCTCGGTCCTTCCATGTTCAGGAAGATTATGACCAAGCCTTTCA
  GTACTACTATCAAGCCACACAGTTTGCTTCATCTTCCTTTGTCCTACCATTTTTTGGTTTGGGACAAATG
  TATATTTATCGAGGTGACAAAGAGAATGCATCTCAGTGCTTTGAGAAAGTTTTGAAAGCTTATCCGAACA
  ATTATGAAACTATGAAAATTCTTGGCTCTCTCTATGCTGCTTCAGAAGATCAGGAGAAACGAGACATAGC
  CAAGGGCCATTTGAAGAAGGTCACAGAACAGTATCCTGATGACGTGGAAGCTTGGATTGAGTTGGCCCAG
  ATCTTAGAACAGACTGACATACAGGGTGCCCTTTCAGCTTATGGAACAGCCACACGCATCCTGCAGGAGA
  AAGTGCAGGCCGATGTCCCTCCCGAGATTTTGAATAATGTTGGGGCCCTCCATTTTAGACTCGGAAACCT
  GGGGGAAGCAAAGAAATACTTCCTGGCCTCACTGGACCGCGCAAAGGCAGAGGCCGAGCATGATGAACAC
  TACTACAATGCCATCTCTGTCACCACATCCTACAATTTAGCCAGGCTCTATGAGGCCATGTGTGAATTTC
  ACGAAGCAGAGAAACTGTACAAAAACATTTTACGAGAGCATCCTAACTACGTTGATTGCTATCTACGCCT
  AGGAGCCATGGCCAGAGATAAAGGAAACTTCTATGAAGCTTCAGACTGGTTTAAGGAAGCCCTTCAGATC
  AATCAGGATCATCCGGATGCCTGGTCTTTGATTGGTAACCTCCATCTGGCAAAACAAGAATGGGGCCCAG
  GTCAGAAGAAGTTTGAGAGGATATTAAAACAGCCAGCCACACAGAGTGACACCTACTCTATGCTGGCCCT
  TGGCAATGTGTGGCTCCAGACTTTGCATCAGCCAACCCGAGACCGAGAAAAGGAAAAGCGCCATCAAGAC
  CGTGCCCTGGCCATCTACAAACAAGTCCTCAGAAATGATGCCAAGAACCTGTATGCTGCCAATGGCATAG
  GAGCTGTCTTGGCCCACAAAGGATACTTCCGTGAAGCTCGTGATGTTTTCGCCCAAGTGAGAGAAGCAAC
  AGCAGATATCAGTGATGTGTGGTTGAACCTAGCACACATCTACGTGGAGCAGAAGCAGTATATCAGCGCC
  GTTCAGATGTATGAGAACTGCCTCAGAAAGTTCTATAAGCACCAAAACACTGAGGTTGTGCTCTACCTGG
  CCCGCGCCCTCTTCAAGTGCGGCAAGCTGCAGGAGTGCAAGCAGACTCTGCTGAAGGCCAGACACGTGGC
  ACCCAGTGACACAGTTCTGATGTTTAATGTGGCCTTGGTCTTGCAAAGATTAGCTACCTCTGTCCTGAAA
  GATGAAAAAAGTAATCTGAAGGAAGTCCTTAATGCTGTGAAAGAGCTGGAGCTCGCACACAGGTACTTCA
  GTTACTTGAGCAAAGTTGGAGATAAAATGAGATTTGATTTGGCCCTTGCTGCCTCTGAAGCCAGGCAGTG
  CTCTGACTTACTGAGCCAGGCCCAGTACCATGTGGCCCGGGCACGCAAGCAGGATGAGGAAGAACGGGAG
  CTGCGGGCCAAGCAGGAGCAGGAAAAGGAGCTCTTAAGACAGAAGCTTCTCAAAGAGCAGGAAGAGAAAC
  GTCTCAGAGAAAAGGAAGAACAAAAGAAACTTTTGGAACAGCGTGCCCAGTATGTGGAGAAGACCAAAAA
  TATCCTTATGTTCACTGGCGAGACTGAAGCAACAAAGGAGAAGAAAAGAGGTGGAGGTGGAGGGCGGCGT
  TCCAAGAAAGGAGGCGAGTTTGATGAATTTGTCAACGATGACACCGACGATGACCTCCCTGTATCCAAAA
  AGAAGAAGAGAAGGAAGGGCAGTGGCAGTGAACAGGAAGGCGAAGAAGAGGAAGGTGGAGAGAGGAAGAA
  GAAGAGGAGGAGAAGACCTCCAAAGGGAGAAGAGGGATCTGAGGAGGAGGAGACAGAAAATGGCCCCAAA
  CCAAAGAAGCGCCGCCCACCGAGAGCAGAGAAAAAGAAGGCTCCCAAGCCAGAACGCCTGCCTCCTTCAA
  TGAAGGGAAAAATAAAATCCAAAGCCATTATATCATCAAGTGATGATTCTTCAGATGAGGATAAACTGAA
  AATTGCTGATGAAGGACATCCCAGGAATAGCAACAGCGATTCTGATGACGACGAGAGGCCCAACAGACGC
  GCCTCATCTGAGAGCGACTCAGATGACAACCAGAACAAGTCTGGCAGCGAGGCAGGCAGCCCTCGGAGGT
  CGGGTAGACAGGAGTCGGATGAGGATTCCGACAGTGACCAGCCGTCCAGAAAGAGAAGGCGCTCCGGCTC
  TGAGCAGTCGGACAATGAGTCTGTGCAGTCAGGGAGAAGCCCCTCCGGAGCTTCTGAGAACGAGAACGAT
  TCTCGCCCGGCTTCTCCAAGTGCAGAATCGGACCACGAATCAGAGCAGGGCTCTGACAACGAGGGCTCTG
  GCCAAGGCTCTGGAAATGAATCAGAACCAGAGGGCTCAAACAACGAGGCCTCAGACCGAGGCTCAGAACA
  TGGGTCAGATGATAGCGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009431
• Insert Size: 3522 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009431.2, NP_033457.2
• RefSeq Size: 4317 bp
• RefSeq ORF: 3522 bp
• Locus ID: 22083
• UniProt ID: Q62018
• Cytogenetics: 7 57.98 cM
• Gene Summary: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (By similarity). Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.[UniProtKB/Swiss-Prot Function]