Pcx (NM_008797) Mouse Untagged Clone

CAT#: MC223821

Pcx (untagged) - Mouse pyruvate carboxylase (Pcx), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcx
• Synonyms: Pc; Pcb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223821 representing NM_008797
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGTTCCAAACAGTTCGAGGGGGCCTGAGGCTCCTGGGTGTCCGCCGATCCTCCTCGGCCCCTG
  TTGCCTCCCCAAATGTCCGGCGTCTGGAGTATAAGCCTATCAAGAAAGTAATGGTGGCCAACAGAGGTGA
  GATTGCCATCCGAGTGTTTCGTGCGTGCACAGAGCTGGGTATCCGCACAGTGGCTGTCTACTCGGAGCAG
  GACACAGGGCAGATGCACAGGCAGAAAGCTGATGAAGCCTACCTTATTGGCCGTGGCCTGGCACCTGTGC
  AGGCCTACCTGCACATTCCAGACATCATCAAGGTGGCCAAGGAAAATGGTGTAGATGCGGTGCATCCTGG
  CTATGGGTTCCTCTCAGAGCGAGCAGACTTTGCCCAGGCCTGCCAAGATGCTGGAGTCCGGTTCATTGGT
  CCAAGCCCAGAGGTGGTCCGCAAGATGGGAGACAAGGTGGAAGCCCGGGCCATTGCCATCGCTGCAGGCG
  TTCCAGTGGTCCCCGGCACGGACTCCCCCATCAGCTCCCTGCACGAGGCGCATGAGTTCTCCAACACCTA
  CGGCTTCCCTATTATCTTCAAGGCCGCCTACGGAGGTGGGGGCCGCGGCATGCGGGTCGTGCATAGCTAT
  GAGGAGTTGGAAGAGAATTACACCCGGGCCTACTCCGAGGCCTTGGCAGCCTTTGGGAATGGAGCGTTGT
  TTGTGGAGAAGTTCATTGAGAAGCCAAGGCACATTGAGGTGCAGATCCTAGGGGACCAGTATGGGAACAT
  CCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGTCGGCACCAGAAGGTGGTAGAGATCGCCCCTGCT
  ACCCACCTGGATCCCCAACTTCGCTCACGTCTCACCAGTGACTCTGTCAAACTTGCCAAGCAGGTAGGCT
  ATGAGAACGCCGGCACTGTGGAGTTCCTGGTGGACAAGCACGGCAAGCACTACTTCATCGAGGTCAATTC
  CCGCCTGCAGGTGGAGCACACGGTCACCGAGGAGATCACAGATGTGGACCTGGTCCATGCTCAGATCCAC
  GTGTCCGAAGGCCGGAGCCTGCCTGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAATGGCTGTGCCA
  TTCAGTGTCGGGTCACCACCGAGGACCCTGCACGCAGCTTCCAGCCAGACACCGGCCGCATTGAGGTTTT
  CCGGAGTGGTGAGGGCATGGGCATCCGCCTGGACAACGCCTCTGCATTCCAGGGCGCTGTCATATCGCCC
  CACTATGACTCTCTGCTCGTCAAGGTCATTGCACACGGCAAAGACCACCCCACAGCTGCCACCAAGATGA
  GCAGAGCCCTGGCCGAGTTCCGTGTCCGAGGTGTAAAGACCAACATCCCCTTCCTGCAGAATGTTCTCAA
  CAACCAGCAGTTCCTGGCAGGCACAGTGGACACCCAGTTCATCGATGAGAACCCTGAGCTGTTCCAGCTT
  CGGCCTGCACAGAACCGGGCCCAGAAGTTGCTACATTACCTCGGACATGTCATGGTGAATGGCCCTACCA
  CTCCAATCCCTGTCAATGTGAGCCCCAGTCCTGTGGATCCTGCTGTTCCTGTGGTGCCCATAGGCCCACC
  TCCAGCTGGTTTCAGGGACATCCTTCTGCGAGAAGGGCCAGAGGGCTTTGCCCGAGCTGTGCGGAATCAC
  CAGGGGCTGCTGTTGATGGACACAACCTTCCGGGATGCCCACCAGTCACTACTGGCCACTAGAGTGCGCA
  CACATGATCTCAAAAAGATTGCGCCCTATGTTGCCCACAACTTCAACAAGCTCTTCAGCATGGAGAACTG
  GGGAGGCGCCACGTTCGACGTTGCCATGCGCTTCCTGTACGAGTGCCCCTGGCGGCGGCTCCAGGAGCTC
  CGGGAGCTTATCCCGAACATCCCGTTCCAGATGCTACTGAGGGGGGCCAATGCTGTGGGCTACACCAACT
  ACCCTGACAACGTGGTCTTCAAGTTCTGTGAGGTGGCCAAAGAGAATGGTATGGACGTCTTCCGAGTCTT
  TGACTCCCTCAACTACTTGCCAAACATGCTGCTGGGCATGGAAGCAGCAGGCAGTGCTGGGGGTGTGGTG
  GAGGCTGCCATCTCATACACGGGGGACGTGGCTGACCCTAGTCGCACTAAATACTCACTGGAGTACTACA
  TGGGCTTAGCTGAAGAACTGGTGCGAGCTGGCACTCACATCCTGTGCATTAAGGACATGGCGGGCCTGCT
  GAAGCCTGCCGCCTGCACCATGCTGGTCAGCTCCCTCCGGGACCGATTCCCCGACCTCCCACTGCACATC
  CATACCCATGATACATCAGGGGCAGGTGTGGCAGCCATGCTGGCCTGTGCACAAGCAGGGGCTGATGTTG
  TGGACGTGGCAGTAGACTCCATGTCTGGGATGACCTCACAGCCAAGCATGGGGGCCCTGGTGGCCTGTAC
  CAAAGGGACTCCTTTGGACACAGAGGTACCCCTGGAGCGTGTGTTTGACTACAGTGAGTACTGGGAAGGG
  GCTCGGGGACTGTACGCAGCCTTCGATTGCACGGCTACCATGAAGTCTGGCAACTCCGACGTGTATGAGA
  ATGAGATTCCAGGGGGCCAGTACACCAACCTGCACTTCCAGGCCCATAGCATGGGGCTTGGCTCCAAGTT
  CAAGGAGGTCAAGAAGGCCTATGTGGAGGCTAACCAGATGCTGGGGGACCTCATCAAGGTGACACCATCC
  TCCAAGATTGTGGGGGACCTGGCCCAGTTCATGGTGCAGAATGGGTTGAGCCGGGCAGAGGCAGAAGCTC
  AGGCAGAAGAGCTGTCCTTCCCCCGCTCTGTGGTGGAGTTCCTGCAGGGCTACATTGGCATTCCCCATGG
  GGGTTTCCCTGAGCCCTTTCGCTCTAAGGTGCTAAAGGACCTGCCAAGAATAGAGGGGCGGCCTGGAGCC
  TCCCTCCCTCCCCTGAACCTGAAGGAGCTGGAGAAGGACCTGATTGATAGGCATGGGGAGGAGGTGACCC
  CAGAGGACGTCCTCTCTGCAGCCATGTACCCTGATGTCTTTGCTCAATTCAAAGACTTCACGGCTACCTT
  CGGCCCCCTGGATAGCCTTAATACTCGTCTCTTTCTTCAAGGACCCAAAATTGCAGAGGAGTTTGAGGTT
  GAGCTGGAACGGGGCAAGACCCTGCACATCAAAGCCCTGGCTGTAAGCGACCTGAACCGTGCTGGCCAGA
  GGCAGGTGTTCTTTGAACTCAATGGGCAGCTTCGATCCATTCTGGTTAAAGACACCCAGGCCATGAAGGA
  GATGCACTTCCATCCCAAGGCTTTGAAGGATGTGAAGGGCCAAATTGGGGCCCCGATGCCTGGGAAGGTC
  ATAGACATCAAGGTGGCAGCAGGGGACAAGGTGGCTAAGGGCCAGCCCCTCTGTGTGCTCAGCGCCATGA
  AGATGGAGACTGTGGTGACTTCGCCCATGGAGGGCACTATCCGAAAGGTTCATGTTACCAAGGACATGAC
  TCTGGAAGGCGACGACCTCATCCTAGAGATTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008797
• Insert Size: 3537 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008797.3, NP_032823.2
• RefSeq Size: 4125 bp
• RefSeq ORF: 3537 bp
• Locus ID: 18563
• Cytogenetics: 19 4.07 cM