Zfp318 (NM_021346) Mouse Untagged Clone

CAT#: MC223769

Zfp318 (untagged) - Mouse zinc finger protein 318 (Zfp318), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp318
• Synonyms: 2610034E08Rik; D530032D06Rik; TZF; Znf318
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223769 representing NM_021346
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCGCAGTGGCTCCCGCTCCTCCGTCTCTTCGCACCGGTCGAAAGACGGCAGCGCGAGCGGCCCTC
  CGCCGGGCCGTCCCGTGGGCGCCTCCTCAGGCCCCACACGCCGCCCCTCGTCGCCGCCGCCGCCCTCTTG
  CTCCTCGCTGCGGCTCCCCGCCCGCCGCCACCGCTCGCCCTCAGGCCACCGCGGCCGCTGGGCCTCGCCG
  TCCCCGCCTCGGGGTCGTCGCGGCTCCCCGTCCCCGCCCCGCGGCCGCCGGGCCTCACCGTCTCCGACCC
  GCGGCCGCCGGGCCTCGCCATCCCCTCCGCGGGGTCGTCGTGGCTCCCCGTCCCCGCCGCGCGCCCGTCG
  CGGCTCCCCGTCGCCACCGCGGAGCCGGCGACACTACCCACCTGGCCTGGGTGGCTTCCGAGGCAGCATT
  CGAGGCGAGTCCCGCGCCGACTTCGCCCGGGACGGCCGCGGAGACCATCCAGGCGGCGGCGGCGGCAGTC
  GGAGACGTTCTCCTGGTCTGTGTTCTGATTCTTCTTTGGAGGAGAGTTTAAGGATCACAGTTGGCAATGA
  CCACTTCTGTGTTAGCACACCAGAGCGGCGGCGGCTCAGTGACCGGCTGGGATCTCCAGTGGATGGTCTG
  CAGGACATGGACAGGGATGACCTGACTGATGATTCTGTCTTCACTCGAAGCTCCCAGTGTTCTCGGGGTC
  TTGAACGATACATTTCTCGGGAGGAGGGACCACTTAGTCCCTTCTTGGGACAACTTGATGAGGACTACCG
  AACAAGAGAAACTTTTCTGCATCGACCTGAGTTTAGTCCCCAGAGCAGTTGTCATGATGAATTGTTGAGG
  GGAACAGAGCGGAATAGAGACAAACTTAAAAGCTCCTCCTATTCCATACGATCTGAGGAAAGAAGCCGGG
  AGGCCAAAAGGCCCCGTTATGATGACACAGAGAAGGTACACAGCTCAGGAGGGGATCACTCAAGTTTTAC
  ATCTGGGACTCGAAACTATCGGCAGCGAAGAAGCAGCCCAAGCCCTAGGTTTCTAGATCCCGAGTTTCGA
  GAACTGGACCTTGCCAGGCGAAAGCGAGAGGAAGAAGAGGAACAAAGTAGGAGCTTGAGTCAAGAGCTGG
  TGGGTGTTGGTGATGATCAGATTGGCTGTTCCATCCCTGGCTTGGCAGGAGTCCTAACCACATCAGAGCC
  TGGATATTCTTTGCAGCGGCCTGAGGAAGTACCTATGATGCCCAAGAAGTCCATTCTGAAGAAACGAATT
  GAAGCGGACATGAAGCCTTCCTTGCAGCTTGAGAGCTTTTCTAGCGGTGCCAGCTCTGGTGAAGATCACC
  CTCTTTATTCTGAACATTCACCTCTTCCTCTAAGTGGTGCTATTGCTGCTTTTACTTCAGAGATTGAAAA
  CAAGGGGACTACAGTGGAGGCTGACCTGAAAGAGCCTCAGAGTAACCTATACCAGTGGGGCCCCCTCCGT
  GAGATACCTAAAGACAACAGTGAAAAATTTGACAGTTTTCTAGGCTTTAAGGAAAAATTGGATTTGAAGG
  CTGAGGGACTTGAGCAACAGACAGATTTCTTACTGCCCCATGAGAGAGCTAGCCAGGACGGCAGTGGCTT
  TTCTCGAATTCTGAGCATGTTGGCTGATCCTACCATTACACAGGAAAAAAGAAGACGTAGCTTCCCTGAT
  ATTGAGGATGAGGAGAAATTTCTCTATGGGGATGAAGAAGAGGATATAAAATCAGAATCTCCACTTAAGT
  CCCTGGAGGACCCCGAGAGTGCAGGTACGAGACAGAAGGCAAACTCTCTGCCCTCAACTCCAGCTGTAAA
  GCTAGAATCACTAGAAGAAAGCAATCCAGAATATGCCAAGATTCACAACTTGCTTAAGACCATAGGGCTG
  GATATTGGAGTAGCAGAAATTGGTAAATTGGCTGCACGAACCCAGGAGAGACTTCATGGCAAGAAGCCAT
  CATCACGCCCCTCAGCTGACCGACGTTTGTCAGCTGACCGACACTTGTCTGGTGACCGACACTTTTCAGC
  TGACCGCTGTTCCTCAGTTGAGCACAGTTTCACAGCTGATTGGCGCTCATCAGACCCTCACAGACCGGAG
  AGTAGGGAGACACATCATAGCAATACTCAGTCCCCAGAAGTATCTCATCCACACCCAGCTTCCCCGGTGG
  ATCCTTACTTGCGCACCAAAAATAGCCCTCCATTCCTAAAGTCTGACCATCCAGTGTGCCATGTTTCAGG
  ACCAGAGGTAGTTGGCAGTGGGTTTCAATCATCTGTTGCTGTTCGGTGCATGTTGCCATCAGCCCCATCT
  ACCCCAATTAGACTTCCTCACTCTGCTGCGTTGTCTCAGTTTCATATACCAGGGGCCTCTCAGTTTGCTG
  CAGCTCGGATACCTCCAAATTATCAGGGATCTGTCATTCCCTCTGCCTCCTTTGATGCATATAGGCACTA
  CATGGCATATGCAGCCTCAAGGTGGCCTATGTATCCTGCCTCTCAACCACCAAGCCACCCTTTATCTGAC
  CCTCATAGGCTATTACCAGTAACCAAACAAGCTGCTCGTAGTCGTCCCAATCTCCGGGTGATCCCTACTG
  TGACTCCTGCAAAGCCCAAACAAGAAATACCAGTACTAGGCTCAATTTCTGTAAAGCGAATTCCTGTTCG
  GGTGTCCATTCCATCACTCATAAAATATAATCCAAAGAAGATCTCTGATGAGAAGAACCGTGCCTCCCAG
  AAGCAGAAGGTTATTGAAGAGAGAGAAAAACTGAAAACTGAACAGGAAGCCCGGCAAAAGAAAATGTTTT
  ATCTCACGACTGAGTTGGAGCGGCTCCATAAACAGCAAGGGGAGATGTTGCGTAAGAAGCGAAGGGAGAA
  GGATGGTCACAAAGATCCACTTCTGATGGAGGTGAGTCGACTTCAGGATAGTATTATGAAGGACATTGCC
  GAGCTACATAAAGAGACAGAAGAAGCAGAAAAGAAGCAGTCTGAACTGGACAAAGTGGCTCAGATCTTGG
  GAATTGATATCTTTGATAAATCCCTGAAATCTTCAAATGACAGTAAAGAGTCTACAGAGAAGCCTGAAAA
  AGAAAAATCTAAGAGCCCAGAAAAAGAGTTGTCACCCTCAAACTCTTCCTCTAGCAACAAGGAATCAAAA
  ATGAATGAAAAGTCCTGTATTAAGAGCCCTAGTTCTACTGAAAGCCTCCAACCAACTGTTAAGCAGTCTG
  ACCAGCCTGTTGCTGCTTATGAGTATTATGATGCTGGCAGTCACTGGTGCAAAGACTGCAATACCACCTG
  TGGGACCATGTTTGACTTCTTCACCCATATGCACAATAAGAAGCACACACAGGGGCAGTTCCAGAAGTCT
  TCACATTTTCAAACGGAAGGACTGAAGCAGATGTTTCTGCTCCAGGAATGCAGGGATAGAAACCACAGAG
  ATTATGGAAATAATGTGCGTCCTTGTGGCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021346
• Insert Size: 3465 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021346.2, NP_067321.2
• RefSeq Size: 3921 bp
• RefSeq ORF: 3465 bp
• Locus ID: 57908
• UniProt ID: Q99PP2
• Cytogenetics: 17 C
• Gene Summary: Isoform 2: Acts as a transcriptional corepressor for AR-mediated transactivation function. May act as a transcriptional regulator during spermatogenesis and in particular, during meiotic division.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks three exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.