Trpm5 (NM_020277) Mouse Untagged Clone

CAT#: MC223768

Trpm5 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpm5
• Synonyms: 9430099A16Rik; LTrpC-5; Ltrpc5; Mtr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223768 representing NM_020277
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAACAACCCAGAGCTCCTGCCCCGGCAGCCCCCCAGATACTGAGGATGGCTGGGAGCCCATCCTAT
  GCAGGGGAGAGATCAACTTCGGAGGGTCTGGGAAGAAGCGAGGCAAGTTTGTGAAGGTGCCAAGCAGTGT
  GGCCCCCTCCGTGCTTTTTGAACTCCTGCTCACCGAGTGGCACCTGCCAGCCCCCAACCTGGTGGTGTCC
  CTGGTGGGTGAGGAACGACCTTTGGCTATGAAGTCGTGGCTTCGGGATGTCCTGCGCAAGGGGCTGGTGA
  AAGCAGCTCAGAGCACAGGTGCCTGGATCCTGACCAGTGCCCTCCACGTGGGCCTGGCCCGCCATGTTGG
  ACAAGCTGTACGTGATCACTCTCTGGCTAGCACATCCACCAAGATCCGTGTAGTGGCCATCGGAATGGCC
  TCTCTGGATCGAATCCTTCACCGTCAACTTCTAGATGGTGTCCACCAAAAGGAGGATACTCCCATCCACT
  ACCCAGCAGATGAGGGCAACATTCAGGGACCCCTCTGCCCCCTGGACAGCAATCTCTCCCACTTCATCCT
  TGTGGAGTCAGGCGCCCTTGGGAGTGGGAACGACGGGCTGACAGAGCTGCAGCTGAGCCTGGAGAAGCAC
  ATCTCTCAGCAGAGGACAGGTTATGGGGGCACCAGCTGCATCCAGATACCTGTCCTTTGCCTGTTGGTCA
  ATGGTGACCCCAACACCCTAGAGAGGATTTCCAGGGCAGTGGAGCAGGCTGCCCCATGGCTGATCCTGGC
  AGGTTCTGGTGGCATTGCTGATGTACTCGCTGCCCTGGTGAGCCAGCCTCATCTCCTGGTGCCCCAGGTG
  GCTGAGAAGCAGTTCAGAGAGAAATTCCCCAGCGAGTGTTTCTCTTGGGAAGCCATTGTACACTGGACAG
  AGCTGTTACAGAACATTGCTGCACACCCCCACCTGCTCACAGTATATGACTTCGAGCAGGAGGGTTCGGA
  GGACCTGGACACTGTCATCCTCAAGGCACTTGTGAAAGCCTGCAAGAGCCACAGCCAAGAAGCCCAAGAC
  TACCTAGATGAGCTCAAGTTAGCAGTGGCCTGGGATCGCGTGGACATTGCCAAGAGTGAAATCTTCAATG
  GGGACGTGGAATGGAAGTCCTGTGACTTGGAAGAGGTGATGACAGATGCCCTCGTGAGCAACAAGCCTGA
  CTTTGTCCGCCTCTTTGTGGACAGCGGTGCTGACATGGCCGAGTTCTTGACCTATGGGCGGCTGCAGCAG
  CTTTACCATTCTGTGTCCCCCAAGAGCCTCCTCTTTGAACTGCTGCAGCGTAAGCATGAGGAGGGTAGGC
  TGACACTGGCCGGCCTGGGTGCCCAGCAGGCTCGGGAGCTGCCCATTGGTCTGCCTGCCTTCTCACTCCA
  CGAGGTCTCCCGCGTACTCAAAGACTTCCTGCATGACGCCTGCCGTGGCTTCTACCAGGACGGGCGCAGG
  ATGGAGGAGAGAGGGCCACCTAAGCGGCCCGCAGGCCAGAAGTGGCTGCCAGACCTCAGTAGGAAGAGTG
  AAGACCCTTGGAGGGACCTGTTCCTCTGGGCTGTGCTGCAGAATCGTTATGAGATGGCCACATACTTCTG
  GGCCATGGGCCGGGAGGGTGTGGCTGCTGCTCTGGCTGCCTGCAAGATCATAAAGGAAATGTCCCACCTG
  GAGAAAGAGGCAGAGGTGGCCCGCACCATGCGTGAGGCCAAGTATGAGCAGCTGGCCCTGGATCTTTTCT
  CAGAGTGCTACGGCAACAGTGAGGACCGTGCCTTTGCCCTGCTGGTGCGAAGGAACCACAGCTGGAGCAG
  GACCACGTGCCTGCACCTGGCCACTGAAGCTGATGCCAAGGCCTTCTTTGCCCATGACGGTGTGCAAGCA
  TTCCTGACCAAGATCTGGTGGGGAGACATGGCCACAGGCACACCCATCCTACGGCTTCTGGGTGCCTTCA
  CCTGCCCAGCCCTCATCTACACAAACCTCATCTCCTTCAGTGAGGATGCCCCGCAGAGGATGGACCTAGA
  AGATCTGCAGGAGCCAGACAGCTTGGATATGGAAAAGAGCTTCCTATGCAGCCGGGGTGGCCAATTGGAG
  AAGCTAACAGAGGCACCAAGGGCTCCAGGCGATCTAGGCCCACAAGCTGCCTTCCTGCTCACACGGTGGA
  GGAAGTTCTGGGGCGCTCCTGTGACTGTGTTCCTGGGGAATGTGGTCATGTACTTCGCATTCCTCTTCCT
  GTTCACCTATGTCCTGCTGGTGGACTTCAGGCCACCACCCCAGGGGCCGTCTGGATCCGAGGTTACCCTC
  TATTTCTGGGTGTTCACACTGGTGCTGGAGGAAATCCGACAGGGCTTCTTCACAGATGAGGACACGCACC
  TGGTGAAGAAATTCACTCTGTATGTGGAAGACAACTGGAACAAGTGTGACATGGTGGCCATCTTCCTGTT
  CATTGTGGGAGTCACCTGTAGAATGGTGCCCTCGGTGTTTGAGGCTGGCAGGACCGTTCTGGCCATTGAC
  TTCATGGTGTTCACACTTCGGCTCATCCACATCTTTGCTATTCACAAGCAGTTGGGTCCTAAGATCATCA
  TTGTAGAGCGAATGATGAAGGATGTCTTCTTTTTCCTCTTCTTCCTGAGCGTATGGCTTGTGGCCTATGG
  TGTGACCACTCAGGCCCTGCTGCATCCCCATGATGGCCGTTTGGAGTGGATTTTCCGCCGTGTGCTATAC
  AGGCCTTACCTGCAGATCTTTGGGCAAATCCCTCTGGATGAAATTGATGAGGCTCGTGTGAACTGTTCTC
  TTCACCCTCTGCTGCTGGAAAGCTCGGCTTCCTGCCCTAATCTCTATGCCAACTGGCTGGTCATTCTCCT
  GCTGGTTACCTTCCTGCTTGTCACTAATGTGCTGCTCATGAACCTTCTGATCGCCATGTTCAGCTACACA
  TTCCAGGTGGTGCAAGGCAATGCAGACATGTTCTGGAAGTTTCAACGCTACCACCTCATCGTTGAATACC
  ATGGAAGACCAGCTCTGGCCCCGCCCTTCATCCTGCTCAGCCACCTGAGCCTGGTGCTCAAGCAGGTCTT
  CAGGAAGGAAGCCCAGCATAAGCGACAACATCTGGAGAGAGACTTGCCTGACCCCTTGGACCAGAAGATC
  ATTACCTGGGAAACGGTTCAAAAGGAGAACTTCCTGAGTACCATGGAGAAACGGAGGAGGGACAGCGAGG
  GGGAGGTGCTGAGGAAAACGGCACACAGAGTGGACTTGATTGCCAAATACATCGGGGGGCTGAGAGAGCA
  AGAAAAGAGGATCAAGTGTCTGGAATCACAGGCCAACTACTGTATGCTCCTCTTGTCCTCTATGACGGAT
  ACACTGGCTCCAGGAGGCACCTACTCAAGCTCTCAGAACTGTGGTTGCAGGAGTCAGCCAGCCTCTGCTA
  GAGACAGGGAGTACCTAGAGTCTGGCTTGCCACCCTCTGACACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020277
• Insert Size: 3477 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020277.2, NP_064673.2
• RefSeq Size: 4383 bp
• RefSeq ORF: 3477 bp
• Locus ID: 56843
• UniProt ID: Q9JJH7
• Cytogenetics: 7 F5
• Gene Summary: Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals.[UniProtKB/Swiss-Prot Function]