Washc5 (NM_153548) Mouse Untagged Clone

CAT#: MC223759

E430025E21Rik (untagged) - Mouse RIKEN cDNA E430025E21 gene (E430025E21Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Washc5
• Synonyms: AL022848; C76463; E430025E21Rik; Kiaa0196; mKIAA0196; strumpellin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223759 representing NM_153548
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGACTTCCTGGCTGAGAATAATCTCTGTGGCCAGGCCATCCTGAGGATCGTCTCCTGTGGCAATG
  CCATCATTGCCGAGGTGCTGCGGCTCTCTGAGTTCATTCCTGCTGTGTTCCTGCTGAAAGACCGGGCTGA
  CCAGCAGAGATATGGAGACATCATCTTTGACTTCAGCTACTTTAAGGGTCCAGAGTTCTGGGAAAGCAAA
  CTGGAAGCCAAGCCTGAGCTGCAGGATTTAGATGAAGAATTTCGGGAAAACAACATAGAAATCGTGACCA
  GATTTTATTTAGCCTTTCAAAGTGTGCATAAATACATTGTAGACTTAAACAGATACCTCGATGACCTCAA
  TGAAGGAGTTTATATTCAGCAAACCTTAGAAACCGTGCTTCTCAGTGAAGATGGAAAACAACTTCTATGT
  GAAGCCCTGTACTTATATGGAGTTATGCTGCTTGTCATCGACCAGAAGATTGAAGGAGAAGTCAGAGAGA
  GAATGCTGGTTTCTTACTATCGATACAGTGCTGCTCGGTCCTCTGCTGACTCAAACATGGATGATATCTG
  TAAGCTGCTACGAAGCACAGGATATTCCAGCCAGCCAGGAGCCAAAAGACCACCCAACTATCCTGAGAGC
  TATTTCCAGAGAGTGCCTATCAACGAGACGTTCATCAGCATGGTCATCGGACGCCTGAGGTCTGATGACA
  TTTACAACCAGGTCTCTGCCTACCCGCTGCCGGAGCACCGCAGCACTGCCCTGGCCAATCAAGCCGCCAT
  GCTCTACGTGATTCTCTATTTTGAACCTTCCATTCTTCACACCCACCAAGCAAAAATGAGAGAGATCGTG
  GATAAGTACTTTCCAGATAACTGGGTAATCAGCATCTATATGGGTATCACTGTTAATCTAGCTGATGCCT
  GGGAACCTTACAAAGCTGCGAAAACTGCTCTCAACAACACCCTTGACCTCGCCAATGTCAAAGAACAGGC
  AAGCAGATACGCTAGTGTCAGCGACAGAGTGCGCGCTCAGGTGCAGCAGTTTCTGAAAGAGGGCTACCTG
  AGGGAGGAGGTGCTTCTGGACAACATCCCCAGGCTGCTGAACTGCCTGAGAGACTGCAATGTCGCCATCC
  GCTGGCTAATGCTCCACACAGCCGACTCAGCCTGTGACCCAAACAACAAGCGCCTTCGTCAAATCAAGGA
  CCAGATTCTGGCAGACTCTAGGTACAATCCCAAGATCCTCTTCCAGTTGCTATTGGACACTGCACAATTC
  GAGTTTATACTCAAAGAGATGTTCAAGCAAATGCTTTCCGAGAAACAGTCCAAGTGGGAGCATTACAAGA
  AGGAGGGCTCTGAGCGCATGACCGAGCTTGCTGACGTCTTTTCAGGGGTGAAACCACTAACCCGAGTGGA
  GAAAAACGAAAATCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAATACTGTCTCTAAATTATGATGAT
  TCTACTGCTGCAGGCAGAAAAACTGTGCAACTAATACAAGCTCTGGAAGAGGTTCAGGAGTTTCACCAGT
  TGGAATCAAATCTACAAGTTTGCCAGTTTCTAGCTGACACCCGAAAGTTTCTCCATCAAATGATCAGAAC
  CATCAACATCAAGGAGGAAGTCCTGATCACAGTGCAGATCATCGGGGACCTTTCCTTTGCCTGGCAGTTA
  ATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAACCCATCCATGGTTACCAAACTCAGAG
  CCACGTTCCTAAAGCTTGCCTCTGCCCTTGACCTTCCCCTGCTACGTATTAATCAAGCAAATAGCCCTGA
  TCTCCTCAGTGTGTCTCAGTATTATTCAGGAGAATTGGTGTCATACGTGAGAAAGGTTTTGCAGATCATT
  CCAGAAAGCATGTTCACATCTCTCCTGAAGATCATAAAGCTTCAGACCCATGACATCATGGAAGTGCCTA
  CACGCCTGGACAAGGACAAGCTGCGGGACTACGCTCAGCTTGGCCCGAGATATGAGGTTGCCAAGCTCAC
  TCACGCCATCTCCATCTTTACTGAGGGCATCTTGATGATGAAAACGACCCTGGTGGGCATCATCAAGGTG
  GATCCCAAGCAGTTGCTGGAAGACGGAATAAGGAAAGAGCTGGTTAAACGCGTGGCTTTCGCCCTTCACC
  GGGGACTGATATTCAACCCTCGTGCCAAGCCAAGTGAGTTGATGCCCAAACTGAAAGAGCTGGGGGCCAC
  CATGGATGGGTTCCATCGCTCCTTTGAGTATATACAGGACTACGTCAGCATTTACGGTCTGAAGATTTGG
  CAGGAGGAAGTCTCTCGTATTATAAATTATAACGTGGAACAGGAGTGTAATAACTTCTTAAGAACAAAGA
  TTCAAGACTGGCAGAGTATGTACCAATCCACTCATATACCAATCCCGAAGTTTGCACCTGTGGATGAGTC
  TATAACATTTATTGGTCGACTCTGCAGAGAAATCTTGCGGATCACAGACCCAAAAATGACATGTTACATA
  GACCAGCTGAACACTTGGTATGACGTGAAAACTCATCAAGAAGTGACCAGCAGTCGCCTCTTCTCAGAAA
  TCCAGACCACCTTGGGGACTTTTGGTCTGAATGGATTGGACAGGCTTCTGTGCTTCATGATTGTGAAAGA
  GCTACAGAATTTCCTCAGTATGTTTCAGAAAATTATCCTGAAAGAGAGAACTGTTCAAGAAACTTTAAAG
  ATGCTCATGAGTGCTGTTAACCCTCTAAAGAGCATAGTGGCAAATTCAAGTAAAGTTTATCTTTCTGCCA
  TCACCAAAACACAGAAGATCTGGAGTGCTTACCTGGAGGCTATAATGAAGGTTGGACAGATGCAGATCCT
  GAGACAACAGATCGCCAATGAGCTAAACTCTTCCTGTCGATTTGATTCCAGACATCTGGCAGCTGCCCTG
  GATAATCTCAATAAGGCTCTCCTGGCAGACATCGAAGCCCACTACCGGGACCCTTCGCTTCCTTACCCCA
  AAGAAGATAACACACTCTTATATGAAATCACAGCCTATCTGGAGGCGGCTGGCATTCACAACCCCCTGAA
  TAAGATTTACATAACAACGAAGCGCTTACCCTATTTCCCGATTGTCAACTTTCTGTTTTTGATCGCGCAG
  TTGCCAAAACTTCAGTATAACAAAAATCTAGGCATGGTCTGCCGGAAGCCTGCCGACCCTGTTGACTGGC
  CACCGCTCGTCCTAGGTCTGCTCACGCTGCTGAAGCAGTTCCACTCCCGGTACACCGAGCAATTCCTGGC
  ACTGATTGGCCAGTTTATTCGCTCCACAATGGAGCAGTGCACAAGCCAGAAGATGCCTGAAATGCCTGCA
  GATGCTGTGGGCGCCCTTCTCTTCCTGGAGGATTATGTTCGGTATACAAAACTCCCCCGAAGGGTGGCTG
  AAGCCCATGTGCCTAACTTCATTTTTGATGAATTCCGAACAGTCCTGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_153548
• Insert Size: 3480 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153548.2, NP_705776.2
• RefSeq Size: 4038 bp
• RefSeq ORF: 3480 bp
• Locus ID: 223593
• UniProt ID: Q8C2E7
• Cytogenetics: 15 D1
• Gene Summary: Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting. May be involved in axonal outgrowth. Involved in cellular localization of ADRB2. Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (By similarity). Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation (PubMed:24998208).[UniProtKB/Swiss-Prot Function]