Xpo4 (NM_020506) Mouse Untagged Clone

CAT#: MC223741

Xpo4 (untagged) - Mouse exportin 4 (Xpo4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Xpo4
• Synonyms: B430309A01Rik; mKIAA1721
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223741 representing NM_020506
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCGGCGGCGCTGGGGCCCCCAGAAGTGATCGCTCAGCTGGAGAACGCGGCCAAAGTTCTGATGG
  CACCACCTTCCATGGTCAGTAATGAGCAGCGCCAGCATGCAGAGCACATATTCCTATCATTTAGGAAGTC
  CAAATCACCATTTGCAGTTTGCAGGCATATTTTGGAAACCAGTAAAGTGGACTATGTCCTTTTTCAAGCT
  GCCACAGCTATAATGGAAGCGGTTGTCCGAGAGTGGGTTCTCTTGGAAAAGGGCAGCATCGAGTCACTGA
  GAACATTCCTCCTCACCTATGTCTTGCAAAGGCCTAACCTCCAAAAGTATGTTCGGGAACAGATTCTATT
  AGCAGTAGCAGTGATTGTAAAAAGAGGATCATTAGATAAGTCAATTGACTGCAAAAGCATATTTCATGAA
  GTCAGCCAGTTGATAAGTAGTGGCAATCCCACTGTGCAAACTCTGGCCTGTTCTATTCTAACTGCTCTAT
  TGAGTGAATTTTCAAGTTCAAGTAAAACTAGCAACATTGGGCTGAGTATGGAATTCCACGGTAACTGCAA
  GCGAGTCTTTCAGGAAGAAGACCTTCGGCAGATCTTCATGTTAACAGTTGGAGTTCTGCAGGAGTTCAGC
  AGACGGGAGAACCTCAGTGCTCAGATGTCCTCAGTGTTTCAGCGGTACCTCGCCCTCGCCAACCAAGTCC
  TGAGCTGGAATTTTCTCCCTCCAAATTTGGGCAGACATTATATAGCTATGTTTGAATCCTCACAAAATGT
  GCTGTTGAAGCCAACAGAGTCCTGGCGGGAGGCTCTTCTGGACAGCAGAGTTATGGAGCTCTTCTTCACT
  GTACATCGAAAAATCAGAGAAGATTCAGATATGGCACAGGATTCCCTGCAGTGCCTTGCCCAGTTAGCGT
  CTCTCCACGGACCCATCTTCCCCGACGAAGGATCCCAGGTTGATTATCTAGCACACTTCATTGAGGGCTT
  GCTCAACACTATTAATGGAATTGAAATAGAAGATTCTGAAGCCGTAGGGATCTCCAGCATTATCAGCAAT
  CTGATAACTGTGTTCCCTCGGAATGTTTTAACTGCCATCCCCAGTGAGCTCTTCTCCTCCTTTGTGAACT
  GCCTCACACACCTCACTTGTTCTTTTGGGCGAAGTGCTGCGTTGGAAGAAGTGCTTGATAAGGATGACAT
  GGTATACATGGAAGCATATGATAAATTGCTGGAGTCCTGGCTAACTTTGGTCCGAGATGACAAGCATTTC
  CATAAAGGCTTTTTCACACAGCATGCAGTTCAGGTGTTCAATTCCTATATTCAATGTCACCTTGCTGCTC
  CAGATGGCACAAGAAATTTGACAGCCAATGGTGTGGCCTCGCGTGAGGAAGAAGAAATAAGTGAGCTCCA
  AGAGGATGATCGTGATCAGTTTTCCGACCAACTAGCCAGCGTAGGAATGCTGGGACGGATTGCTGCCGAA
  CACTGTATGCCTCTTCTGACAAGTTTATTAGAAGAAAGGGTAACAAGACTCCATGGCCAGTTACAACGGC
  ATCAGCAACAATTCCTTGCTTCACCCGGATCAAGCACCATTGACAACAAAATGCTTGACGATCTTTACGA
  GGATATTCACTGGCTTATTCTAGTTACAGGCTACCTCTTAGCTGATGATACTCAGGGAGAGACTCCGCTA
  ATACCTCCAGAAATAATGGAATATTCCATTAAGCATTCATCTGAAGTTGACATTAATACAACACTTCAAA
  TTTTGGGATCTCCAGGAGAAAAGGCTTCTTCCATCCCAGGGTACAGCAGAACAGATTCTGTGATTAGGCT
  GTTGTCTGCTGTGCTAAGAGTCTCAGAAGTTGAATCTCGAGCAATAAGAGCAGATCTCACTCATCTACTA
  AGCCCTCAGATGGGCAAAGATATTGTTTGGTTTCTAAAACGCTGGGCCAAGACTTACCTCCTGGTAGATG
  AAAAACTGTATGATCAGATAAGTTTGCCATTGAGCACAGCATTTGGAGCGGATACAGAGGGTTCCCAGTG
  GATTATCGGCTACCTCTTACAAAAGGTCATCAGCAATCTGTCGGTATGGAGCAGTGAACAGGACCTCGCG
  AATGACACTGTTCAGCTCCTTGTCACTTTGGTAGAAAGAAGAGAAAGGGCAAACTTAGTAATTCAGTGTG
  AAAACTGGTGGAACCTAGCAAAGCAGTTTGCAAGCCGAAGCCCACCTCTTAACTTCCTGTCCAGTCCAGT
  GCAGAGGACACTGATGAAGGCTCTGGTCTTAGGAGGCTTTGCACATATGGACACAGAAACCAAGCAGCAG
  TATTGGACTGAGGTTCTGCAGCCTCTTCAGCAGAGGTTCCTGAGAGTGATCAACCAAGAGAACTTTCAGC
  AGATGTGTCAGCAGGAGGAAGTCAAGCAGGAGATCACTGCCACATTGGAGGCCCTGTGTGGCATTGCTGA
  GGCCACCCAGATCGACAATGTAGCCATCCTTTTTAATTTTTTAATGGACTTCCTTAACAATTGCATTGGT
  TTAATGGAAGTTTACAAAAATACTCCAGAAACTGTCAATCTCATTATAGAAGTTTTTGTTGAAGTTGCAC
  ATAAACAAATATGCTATCTTGGAGAGTCCAAAGCTATGCATTTATACGAAGCCTGCCTTACTTTGTTACA
  AGTGTATTCTAAGAATAACTTAGGGCGGCAAAGGATAGACGTCACGGCGGAGGAAGAGCAGTACCAAGAC
  CTGCTTCTCATCATGGAGCTTCTCACTAACCTTCTCTCAAAGGAGTTCATCGACTTTAGTGACACAGATG
  AAGTGTTTAGAGGACATGAGCCAGGACAAGCAGCAGGCAGATCTGTATCGGCAGCTGATGTCGTTTTATA
  TGGAGTAAACCTAATTCTGCCCTTAATGTCACAAGATCTTTTGAAGTTTCCAACCCTTTGTAATCAATAT
  TACAAACTGATCACATTTATTTGTGAGATTTTTCCTGAAAAGATACCGCAGCTTCCTGAGGATCTGTTTA
  AGAGTCTGATGTACTCCCTAGAACTAGGGATGACATCAATGAGTTCAGAAGTATGCCAGCTTTGTCTCGA
  GGCCTTGACACCATTAGCTGAACAATGTGCTAAAGCACAAGAAACGGATTCACCACTTTTTCTAGCAACA
  CGACACTTCCTTAAGCTGGTTTTTGATATGCTGGTCTTGCAAAAGCACAACACGGAGATGACCACTGCAG
  CAGGCGAAGCATTCTACACATTGGTGTGTTTGCACCAGGCTGAATATTCTGAACTGGTTGAGACATTACT
  GTCAAGTCAACAAGACCCAGTCATTTACCAGAGATTAGCAGATGCCTTCAACAAGCTCACAGCGAGCAGC
  ACTCCTCCTGCCCTGGACCGGAAGCAGAAGATGGCCTTCCTAAAGAGTTTAGAAGAATTTATGGCAAATG
  TTGGTGGTCTCCTTTGTGTAAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020506
• Insert Size: 3456 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020506.1, NP_065252.1
• RefSeq Size: 3456 bp
• RefSeq ORF: 3456 bp
• Locus ID: 57258
• UniProt ID: Q9ESJ0
• Cytogenetics: 14 C3
• Gene Summary: Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.