Megf10 (NM_001001979) Mouse Untagged Clone

CAT#: MC223726

Megf10 (untagged) - Mouse multiple EGF-like-domains 10 (Megf10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Megf10
• Synonyms: 3000002B06Rik; Gm331
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223726 representing NM_001001979
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATTTCTTCAAGTTCGTGCCTGGGCCTCATCTGCTCACTGCTCTGTCACTGGGTGGGGACAGCAT
  CCTCCCTGAACCTGGAAGACCCCAACGTATGCAGCCACTGGGAAAGCTACTCGGTGACTGTGCAGGAGTC
  GTATCCACATCCCTTCGATCAGATCTACTACACAAGCTGCACCGACATCCTGAACTGGTTTAAATGCACA
  CGGCACAGAATCAGCTACCGGACAGCCTACCGCCACGGGGAGAAAACCATGTATAGACGCAAATCCCAGT
  GTTGCCCAGGATTTTATGAAAGCCGAGACATGTGTGTCCCTCACTGTGCTGATAAATGTGTCCATGGTCG
  CTGCATTGCTCCAAACACCTGTCAGTGTGAGCCTGGCTGGGGTGGGACCAACTGTAGCAGTGCTTGTGAT
  GGTGATCACTGGGGGCCTCACTGCAGCAGCCGATGCCAGTGCAAAAACAGAGCTTTGTGTAACCCCATCA
  CCGGTGCTTGCCACTGCGCTGCGGGCTACCGGGGATGGCGCTGCGAGGACCGTTGTGAACAGGGCACGTA
  CGGTAACGACTGTCACCAAAGATGCCAGTGTCAGAATGGAGCGACCTGTGACCACATCACTGGGGAATGC
  CGTTGTTCACCTGGGTACACTGGAGCCTTCTGTGAGGATCTTTGTCCTCCTGGCAAACATGGTCCACATT
  GTGAGCAGAGGTGTCCCTGCCAAAATGGGGGTGTGTGCCACCATGTCACTGGAGAGTGCTCTTGCCCTTC
  TGGTTGGATGGGCACAGTGTGTGGTCAGCCCTGCCCTGAGGGTCGCTTTGGAAAGAACTGTTCCCAAGAA
  TGCCAGTGTCACAATGGAGGAACGTGTGATGCTGCCACAGGCCAGTGTCACTGCAGCCCAGGATACACAG
  GGGAACGGTGTCAGGACGAATGTCCTGTTGGGAGCTATGGAGTTCGCTGTGCTGAGGCCTGCAGGTGTGT
  CAACGGAGGCAAGTGTTACCACGTGAGTGGTACATGCCTGTGCGAAGCAGGCTTTTCGGGTGAACTTTGC
  GAGGCGCGCCTGTGTCCCGAGGGGCTTTACGGCATCAAATGTGACAAGCGGTGCCCCTGCCACCTGGACA
  ACACTCACAGCTGTCATCCCATGTCTGGAGAGTGTGGCTGCAAGCCGGGTTGGTCGGGACTGTACTGTAA
  TGAAACATGCTCCCCTGGATTCTACGGGGAGGCTTGCCAACAGATCTGCAGCTGCCAGAACGGGGCGGAC
  TGCGACAGTGTGACTGGAAGGTGTGCCTGCGCTCCAGGATTCAAAGGGACTGACTGCTCTACTCCGTGTC
  CTCTGGGACGCTACGGGATAAATTGTTCTTCTCGCTGTGGCTGTAAAAATGATGCTGTCTGTTCTCCTGT
  GGATGGATCATGTATCTGTAAGGCAGGCTGGCACGGGGTGGACTGTTCCATCCGCTGCCCCAGTGGCACA
  TGGGGCTTTGGCTGTAACCTAACGTGTCAGTGCCTCAATGGCGGTGCCTGCAACACGCTGGATGGGACCT
  GCACCTGTGCGCCCGGATGGCGAGGCGCGAAGTGTGAATTTCCCTGCCAGGATGGCACTTATGGGCTGAA
  CTGTGCCGAGCGCTGTGACTGCAGCCATGCAGATGGCTGTCACCCCACTACAGGCCATTGCCGCTGCCTC
  CCTGGATGGTCAGGTGTGCACTGTGACAGTGTGTGCGCTGAGGGACGCTGGGGTCCTAACTGCTCGCTGC
  CCTGCTACTGTAAAAATGGGGCTTCGTGTTCTCCGGATGATGGCATCTGTGAGTGTGCACCCGGATTCCG
  AGGCACCACTTGCCAGAGAATCTGCTCCCCCGGTTTTTATGGACATCGCTGTAGCCAGACCTGCCCGCAG
  TGTGTGCACAGCAGTGGGCCCTGCCACCACATCACGGGCCTGTGTGACTGCTTACCTGGCTTCACCGGTG
  CCCTGTGCAATGAAGTGTGTCCCAGTGGCAGATTTGGGAAAAACTGTGCAGGCGTTTGTACTTGCACCAA
  CAATGGCACCTGTAACCCCATCGACAGATCCTGCCAGTGTTACCCAGGCTGGATTGGCAGTGACTGCTCC
  CAGCCCTGTCCACCTGCGCACTGGGGTCCGAACTGCATCCACACCTGCAACTGCCACAACGGAGCCTTTT
  GCAGCGCCTATGATGGGGAATGCAAATGCACTCCTGGCTGGACGGGGCTCTACTGCACTCAGAGATGCCC
  TCTGGGCTTCTATGGTAAGGACTGTGCACTGATATGCCAATGTCAAAACGGAGCTGACTGCGACCATATC
  TCGGGGCAGTGTACCTGCCGCACGGGATTCATGGGACGGCACTGTGAACAGAAGTGCCCTGCGGGAACAT
  ACGGCTATGGCTGTCGCCAGATCTGTGACTGTCTGAACAACTCCACCTGTGACCACATCACTGGCACGTG
  TTACTGTAGCCCAGGATGGAAAGGGGCACGATGTGACCAAGCTGGGGTTATCATCGTGGGCAATCTGAAC
  AGCTTAAGCCGGACCAGCACCGCCCTTCCTGCCGATTCCTATCAGATCGGGGCCATCGCGGGCATCGTGG
  TCCTCGTTCTTGTTGTGCTCTTCCTGCTGGCGCTGTTCATCATCTACAGACACAAGCAGAAGAGGAAGGA
  ATCAAGCATGCCGGCCGTGACCTACACCCCCGCCATGAGGGTCATCAATGCAGACTATACCATCGCAGAA
  ACCCTGCCTCACAGCAATGGTGGAAATGCCAACAGCCACTACTTTACCAATCCCAGTTATCACACACTTA
  GCCAGTGTGCCACATCCCCTCATGTGAACAATAGGGACAGGATGACCATTGCAAAGTCAAAAAACAATCA
  GCTGTTTGTGAATCTTAAAAATGTGAATCCAGGGAAGAGAGGGACATTGGTGGACTGCACTGGGACATTG
  CCAGCTGACTGGAAGCAAGGAGGCTACCTCAATGAGCTTGGTGCTTTCGGGCTGGACAGAAGCTACATGG
  GAAAGTCCTTAAAAGATCTGGGGAAGAACTCTGAATATAATTCAAGCACTTGCTCCTTAAGCAGCTCTGA
  AAACCCATATGCCACCATTAAAGACCCGCCTGCACTCCTGCCTAAAAGCTCCGAGTGCGGCTACGTGGAG
  ATGAAGTCGCCGGCGCGGAGAGACTCCCCATATGCAGAGATCAACAACTCAACTCCAGCCAACAGGAATG
  TCTATGAAGTCGAACCTACAGTGAGCGTTGTGCAAGGAGTATTCAGCAACAGCGGTCACGTCACCCAAGA
  CCCATATGACCTTCCAAAGAACAGTCACATCCCTTGCCATTATGACCTGCTGCCAGTAAGGGACAGTTCA
  TCCTCCCCAAAGAGAGAGGATGGTGGTGGCAGCAACAGCACCAGCAGCAACAGCACCAGCAGCAGCAGCA
  GCAGCAGTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001979
• Insert Size: 3444 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001979.2, NP_001001979.1
• RefSeq Size: 7514 bp
• RefSeq ORF: 3444 bp
• Locus ID: 70417
• UniProt ID: Q6DIB5
• Cytogenetics: 18 D3
• Gene Summary: Membrane receptor involved in phagocytosis by macrophages and astrocytes of apoptotic cells. Receptor for C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells (PubMed:27170117). Cooperates with ABCA1 within the process of engulfment (By similarity). Promotes the formation of large intracellular vacuoles and may be responsible for the uptake of amyloid-beta peptides (PubMed:20828568). Necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum (PubMed:27170117). Plays role in muscle cell proliferation, adhesion and motility. Is also an essential factor in the regulation of myogenesis. Controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway (PubMed:28498977). May also function in the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. Mosaics provide a mechanism to distribute each cell type evenly across the retina, ensuring that all parts of the visual field have access to a full set of processing elements (PubMed:22407321).[UniProtKB/Swiss-Prot Function]