Trim33 (NM_053170) Mouse Untagged Clone

CAT#: MC223715

Trim33 (untagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim33
• Synonyms: 8030451N04Rik; AI413936; Ecto; Tif1g
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223715 representing NM_053170
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAAACAAAGGCGGCGGTGAGGCAGAGAGTGGCGGCGGGGGCAGCGGTAGCGCGCCGGTAACCG
  CCGGGGCCGCCGGGCCCACGGCGCAGGAGGCGGAGCCGCCTCTCGCCGCGGTGCTGGTGGAGGAGGAGGA
  GGAGGAAGGCGGCAGGGCTGGCGCGGAGGGCGGTGCGGCTGGGCCCGACGACGGGGGGGTGGCCGCGGCC
  TCCTCAAGCTCGGCCCCGGCCGCCTCGGTCCCCGCGGCCTCAGTGGGCTCCGCGGTCCCCGGAGGCGCGG
  CATCGACGCCGGCTCCAGCTGCTGCCCCAGCCCCGGCCCCGGCCCCGGCCCCGGCCCCGGCCCCCGCTCC
  CGCCCCGGCTCCTGGTTCCTCGTCGGGGCCACCTCTCGGACCACCAGCCTCGCTTCTGGACACCTGCGCG
  GTGTGTCAGCAGAGCTTGCAGAGCCGGCGCGAGGCGGAGCCCAAGCTGCTCCCCTGCCTGCACTCCTTCT
  GCCTGCGCTGTCTGCCGGAGCCGGAGCGCCAGCTCAGCGTGCCCATCCCTGGGGGCAGCAACGGTGACGT
  CCAGCAAGTTGGTGTAATACGGTGTCCAGTATGTCGTCAAGAATGTAGACAGATAGATCTTGTGGATAAT
  TATTTTGTGAAAGACACATCTGAAGCTCCTAGCAGCTCTGATGAGAAATCAGAACAGGTATGTACTAGTT
  GTGAAGATAATGCAAGTGCAGTTGGTTTTTGTGTAGAATGTGGAGAGTGGCTGTGTAAGACATGCATTGA
  AGCACATCAAAGAGTAAAATTCACTAAAGACCACCTGATCAGGAAAAAAGAAGATGTCTCAGAGTCTGTT
  GGAACATCTGGTCAGCGCCCAGTGTTTTGTCCTGTACATAAACAAGAGCAGTTGAAACTATTCTGTGAAA
  CGTGTGATAGATTGACGTGTAGAGACTGTCAACTTTTGGAGCACAAAGAACATAGGTATCAGTTTTTAGA
  AGAGGCTTTTCAAAATCAGAAAGGTGCAATTGAAAATCTGTTGGCTAAGCTTCTTGAAAAGAAGAATTAT
  GTTCATTTTGCAGCTACACAAGTGCAGAATAGGATTAAAGAAGTAAATGAGACTAACAAACGTGTAGAAC
  AGGAAATTAAAGTGGCCATCTTCACCCTTATCAATGAAATTAATAAGAAAGGAAAATCTCTCTTGCAACA
  GCTAGAGAATGTTACAAAAGAAAGACAGATGAAGTTACTACAGCAGCAGAATGATATCACAGGCCTCTCC
  CGGCAGGTGAAGCATGTTATGAACTTTACAAACTGGGCCATTGCAAGTGGCAGCAGCACGGCCCTACTTT
  ATAGCAAGCGTCTGATTACTTTTCAGTTACGTCATATTTTGAAAGCCCGATGTGATCCTGTTCCTGCTGC
  CAATGGAGCAATTCGTTTCCATTGTGATCCCACCTTCTGGGCCAAAAATGTAGTGAATTTAGGTAATCTA
  GTAATAGAGAGTAAGCCAGCTCCTGGTTATACTCCTAATGTTGTAGTTGGGCAAGTTCCTCCAGGGACAA
  ACCACATTAGTAAAACCCCTGGACAGATTAATTTAGCACAGCTTCGACTCCAGCACATGCAGCAACAAGT
  GTATGCACAGAAACATCAGCAGTTACAACAGATGAGACTGCAGCAACCACCAGCTCCTATACCAACAACA
  ACAGCAACAACCCAGCAGCATCCTAGGCAAGCAGCCCCACAGATGTTACAACAACAGCCTCCAAGATTGA
  TCAGTGTGCAAACAATGCAAAGAGGCAACATGAACTGTGGAGCATTTCAAGCCCATCAAATGAGACTGGC
  TCAGAATGCTGCCAGAATACCAGGGATCCCCAGGCACAGTGCCCCTCAGTATTCCATGATGCAGCCACAT
  CTCCAAAGACAACATTCAAATCCAGGACATGCTGGACCGTTTCCTGTTGTATCAGCACACAACCCAATCA
  ACCCAACAAGTCCCACCACAGCAACTATGGCAAATGCAAATCGAGGTCCCACCAGCCCGTCTGTTACAGC
  AATAGAATTAATACCCTCAGTCACCAATCCAGAAAACCTTCCATCGTTGCCAGATATCCCACCTATACAG
  TTGGAAGATGCTGGCTCAAGTAGTTTAGATAATCTACTGAGTAGATATATCTCAGGCAGTCATCTACCCC
  CTCAGCCTACAAGTACCATGAATCCTTCCCCAGGACCCTCTGCGTTATCACCTGGATCTTCAGGCTTATC
  CAATTCTCACACACCTGTGAGACCCCCTAGCACGTCTAGTACTGGCAGTCGAGGCAGCTGCGGATCATCA
  GGAAGAACTGCTGAGAAAAGTGCTCATAGTTTCAAGTCTGATCAGGTGAAGGTCAAGCAGGAACCTGGGA
  CTGAAGAAGAGATATGCAGCTTTTCAGGAGCTGTGAAACAAGAGAAGACAGAGGATGGCAGGAGGAGTGC
  CTGTATGTTGAGCAGTCCTGAGAGTAGTTTGACACCACCTCTTTCAACTAACCTTCACCTAGAGAGTGAG
  CTGGATACATTAACAGGCCTGGAGAACCACGTGAAGACTGAACCTACAGATATCAGTGAAAGCTGCAAAC
  AGTCAGGACTCAGTAACCTAGTCAATGGAAAGTCTCCAATTCGAAACCTCATGCACAGGTCGGCAAGGAT
  TGGAGGGGATGGCAATAGCAAAGATGATGACCCAAATGAAGACTGGTGTGCCGTCTGCCAGAACGGAGGG
  GATCTTTTGTGTTGTGAGAAATGTCCGAAGGTCTTTCATCTTACCTGTCATGTTCCAACACTTCTCAGCT
  TTCCAAGTGGAGACTGGATATGCACATTTTGCAGAGATATTGGGAAGCCAGAAGTTGAATATGATTGTGA
  TAATATGCAGCATAGTAAGAAGGGGAAAACTGCACAAGGCTTAAGCCCTGTGGACCAAAGGAAATGTGAA
  CGTCTTCTGCTTTACCTCTATTGTCACGAATTAAGTATTGAATTCCAGGAGCCTGTTCCTGTTTCGATAC
  CAAACTACTATAAAATTATAAAGAAACCGATGGATTTATCAACGGTGAAGAAGAAGCTTCAGAAAAAACA
  TTCCCAGCATTACCAAATCCCAGATGACTTTGTGGCTGATGTCCGTTTGATCTTCAAGAACTGTGAAAGG
  TTTAATGAAATGATGAAAGTTGTTCAAGTTTATGCAGACACACAAGAGATTAATTTGAAGGGTGATTCAG
  AAGTAGCTAAGGCAGGGAAAGCAGTTGCATTGTACTTTGAAGATAAACTCTCAGAGATCTACTCAGACAG
  GACCTTCACTCCTTTGCCAGAGTTTGAGCAGGACGAAGATGATGGTGAAGTAACAGAAGACTCTGACGAA
  GATTTTATACAGCCTCGTAGAAAACGCCTAAAGTCAGACGAGAGACCAGTACATATAAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053170
• Insert Size: 3423 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053170.3, NP_444400.2
• RefSeq Size: 8870 bp
• RefSeq ORF: 3423 bp
• Locus ID: 94093
• UniProt ID: Q99PP7
• Cytogenetics: 3 F2.2
• Gene Summary: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.