Pde3a (NM_018779) Mouse Untagged Clone

CAT#: MC223711

Pde3a (untagged) - Mouse phosphodiesterase 3A, cGMP inhibited (Pde3a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde3a
• Synonyms: A930022O17Rik; C87899
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223711 representing NM_018779
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCGGGGCGAGGCCGCCCAAGACCTAGCCAAGCCGGGCCTCGGAGGGGCGAGTCCCGCCCGTG
  TGGCAAGAGGGAACCACCGCCATCGTGGCGAGTCCTCTCCATCCCCTCGGGGCTCCGGCTGTTGTTGGAG
  AGCCCTGGCGCTGCAGCCGCTCCGGCGCTCGCCTCAACTCTCCTCGGCGCTGTGCGCGGGCTCCCTGTCC
  GTGCTGCTGGCGCTGCTGGTGAGGCTGGTCGGCGGGGAGGTCGGAGGCGAGCTGGAGAAGAGTCAGGAGG
  CGGCGGCCGAGGAGGAGGAGGAGGAAGGAGCCCGAGGGGGCGTCTTCCCGGGCCCTCGGGGAGGTGCTCC
  CGGGGGCGGCGCGCAGCTCAGCCCTTGGCTGCAGCCGGCCGCGCTGCTCTTCAGTCTCCTGTGCGCCTTC
  TTCTGGATGGGCTTGTGCCTGCTGCGCGCCGGGGTGCGCCTGCCTCTGGCCGTCGCGCTGTTGGCCGCCT
  GCTGCGCCGGGGAAGCGCTGGTGCAGCTCTCCTTGGGCGTGGGGGACGGTCGCCTGCTGTCGCTGCCCGC
  CGCGGGGGTCCTGCTCAGCTGCCTAGGTGGCGCGACATGGCTGGTGCTGAGGCTGAGGCTGGGCGTCCTC
  ATGGTCGCCTGGACTAGCGTGCTTAGGACGGTGGCCCTGGTTTCTCTGGAGAGGTTCAAGGTCGCCTGGA
  GACCTTACCTGGCATACTTGGCGGCCGTGCTTGGGCTGCTGCTGGCCAGGTACGCCGAGCAGATCCTGCC
  GCAGTGCTCCGGGCCGGCTCCGCCCAGGGAGCGGTTTGGGTCCCAGTTGAGCGCGAGGACCAAGGAAGAG
  ATTCCGGGGTGGAAGAGGAGGAGGCGGTCCAGCTCGGTGGTGGCCGGCGAGATGTCCGGCTGCAGTGGCA
  AGTCTCACCGGAGGACCTCCCTGCCCTGCATACCCAGGGAGCAGCTCATGGGCCATTCAGAATGGGACCA
  CAAGAGAGGGCCACGAGGATCCCAGTCAGGAACCAGCATCACTGTGGATATTGCTGTGATGGGTGAGGCC
  CATGGCCTCATTACCGACCTCCTGGCCGACCCTTCTCTGCCCCCGAATGTGTGTACATCCTTGAGGGCCG
  TAAGCAACTTGCTCAGCACCCAGCTCACCTTCCAGGCCATCCACAAGCCCAGAGTGAACCCCACCGTGAC
  CTTCAGTGAGAACTATACCTGCTCGGACTCTGAGGAAGGCTTGGAGAAAGACAAGCAGGCCATTTCCAAG
  CGCCTGAGAAGAAGTTTGCCTCCAGGCTTACTGAGAAGAGTCTCGTCAACTTGGACAACTACCACCTCTG
  CCACAGGTCTGCCCACCTTGGAGCCTGCGCCAGTGCGGAGGGACCGCAGTGCCAGCATAAAGCCGCACGA
  AGCCCCTTCACCCAGTGCTGTCAATCCTGACTCTTGGAATGCCCCAGGGTTGACGACTCTCACCAAAAGC
  AGATCCTTCACTTCATCCTATGCTGTCTCTGCAGCTAACCATGTAAAAGCTAAAAAGCAAAACAGGCCAG
  GTGGCCTTGCTAAAATTTCCCCCGTCCCCTCACCCTCCTCCTCGCCTCCCCAGGGATCACCTGCCAGCAG
  TCCTGTCAGCAACAGTGCTTCACAACAGTTCCCAGAATCCCCTGAAGTAACCATCAAGCGAGGGCCTGGA
  TCTCACAGGGCCTTAACTTACACCCAAAGTGCCCCTGACCTGTCCCCTCAGATCCCACCCCCGTCTGTCA
  TATGTAGCAGCTGTGGCAGACCTTATTCGCAAGGGAATCCTGCTGATGGACCCTCAGAGAGAAGTGGCCC
  AGCCATGCTGAAACCAAACAGAACAGATGACACTTCTCAAGTTACCTCCGATTATGAGACCAACAACAAC
  AGTGACAGCAGTGACATTCTGCAGAATGAAGAGGAAGCTGAGTGCCAGAGAGAACCCCAGAGGAAAGCAT
  CGGCTTGTGGCACCTATACTTCCCAGACCATGATCTTCCTGGACAAACCAATTCTTGCTCCAGAACCCCT
  GGTCATGGATAACCTGGACTCAATTATGGATCAGTTGAACACCTGGAATTTTCCAATTTTTGATTTAATG
  GAAAATATAGGAAGAAAATGTGGCCGTATTCTGAGCCAGGTATCATACAGACTGTTTGAAGACATGGGGC
  TCTTTGAAGCCTTTAAAATCCCGGTTAGGGAGTTTATGAATTACTTTCATGCTTTGGAGATCGGATACAG
  GGACATTCCTTATCATAACAGAATCCATGCCACCGATGTTCTACACGCTGTGTGGTATCTCACAACACAG
  CCGATTCCTGGCCTCCCAAGTGTGATTGGTGATCACGGCTCAGCAAGCGACTCCGATTCTGACAGTGGAT
  TTACACACGGACACATGGGATATGTGTTTTCAAAAATGTATCACGTGCCAGATGACAAATATGGATGCCT
  GTCTGGGAATATTCCAGCCCTAGAGTTGATGGCTCTGTACGTTGCTGCAGCCATGCATGACTACGATCAC
  CCAGGGAGGACAAATGCCTTCCTGGTTGCCACTAGTGCCCCTCAGGCGGTGCTATACAACGACCGTTCTG
  TTCTGGAGAACCATCACGCCGCTGCAGCCTGGAATCTCTTCATGTCCCGGCCAGAGTATAACTTCTTAGT
  TAACCTGGACCACGTGGAATTCAAGCACTTCCGCTTCCTAGTCATTGAAGCGATTTTGGCTACTGACCTG
  AAGAAACACTTTGACTTTGTAGCCAAATTTAACGCCAAGGTAAACGATGATGTCGGGATAGATTGGACCA
  ATGAAAACGACCGTCTCCTGGTGTGTCAGATGTGTATAAAGCTGGCTGACATCAACGGGCCTGCTAAATG
  CAAAGAACTCCACCTGCGGTGGACAGAGGGCATCGCCAGCGAGTTTTATGAGCAGGGCGATGAAGAAGCC
  AGCCTCGGCTTGCCCATAAGTCCCTTTATGGACCGCTCTGCCCCGCAGCTGGCCAATCTTCAGGAATCCT
  TTATCTCACACATCGTGGGTCCTCTCTGCCACTCCTATGACTCTGCGGGACTCATGCCAGGAAAATGGGT
  AGATGACAGTGATGATTCCGGAGACACCGATGACCCGGAAGAAGAGGAGGAAGAGGCTGAAACACCACAC
  GAGGACGAAGCCTGTGAAAGCAGTATAGCTCCCCGAAAGAAAAGTTTCAAAAGAAGGAGAATCTACTGCC
  AAATAACTCAGCACCTTCTGCAGAACCACATGATGTGGAAGAAAGTGATTGAAGAGGAACAGTGTTTATC
  AGGCACAGAGAACCAGTCCCTGGACCAGGTCCCTCTGCAGCATCCCTCAGAGCAGATCCAGGCTATCAAG
  GAGGAAGAGGAAGAGAAGGGGAAGCCAAGAGCAGAGGAGACCCTGGCTCCACAGCCAGACCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_018779
• Insert Size: 3426 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018779.1, NP_061249.1
• RefSeq Size: 4205 bp
• RefSeq ORF: 3426 bp
• Locus ID: 54611
• UniProt ID: Q9Z0X4
• Cytogenetics: 6 G2
• Gene Summary: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.[UniProtKB/Swiss-Prot Function]