Usp43 (NM_173754) Mouse Untagged Clone

CAT#: MC223695

Usp43 (untagged) - Mouse ubiquitin specific peptidase 43 (Usp43), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp43
• Synonyms: 9130017K11Rik; B930051C24; C630032K07Rik; RP23-464J2.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223695 representing NM_173754
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCGGGCGTGGGGAACGCACTGGGAGAGGGGCCGCCCGCGCCCCGGCCCCGCCGCCGCCGTTCCC
  TTCGCCGCCTGCTTAACCGCTTCCTGCTAGCTCTGGGCAGCCGCTCCCGCTCGGGGGACTCGCCGCCCCG
  GCCCCCGGCCCAGCCCAGTCCCTACGATGGCGATGGAGAAGGGGGCTTTGCCTGCGCCCCGGGCCCGGCA
  CCAGCCTCTGCCGGGAGTCCAGGGCCAGATCGTCCTCCGGGATCCCAACCCCAGATATCTTCGGGTGACG
  GGGCGCGTCCGCCCGGCGCTCAGGGCTTGAAGAACCATGGCAACACCTGTTTCATGAACGCGGTGGTGCA
  GTGTCTCAGCAACACCGACCTGCTGGCCGAGTTCCTAGCGCTGGGGCGCTACCGGGCGGCGCCGGGCCGC
  GCCGAGGTCACCGAGCAGCTGGCGGCGCTGGTGCGCGCGCTCTGGACGCGCGAATACACGCCCCAACTTT
  CCGCAGAGTTCAAGAATGCTGTTTCTAAGTATGGCTCCCAGTTCCAAGGCAATTCCCAGCACGATGCCCT
  GGAATTCCTGCTCTGGCTTCTGGATCGTGTCCACGAGGACTTGGAAGGCTCAGCTCACGGGCTGGTATCT
  GAGCAGCTGCCCCCAGAAGTGAGTAAGATCTCAGAGGACCTCCGGCCATCTGCAGCTCCTACCTCCTTAG
  GGCCCAGCTTTGTGCAGAGCCACTTTCAAGCACAGTACAGGTCTTCCTTGACTTGTCCACACTGCCTAAA
  ACAAAGCAACACCTTCGACCCTTTCCTGTGTGTGTCCCTTCCCATCCCCTTGCGCCAGACGAGATTCTTG
  AGTGTCACTTTGGTGTTCCCCTCAAAGAGCCAGCGGTTTCTGCGTGTTGGCTTGGCCGTGCCAATCCTCA
  GCACAGTGGCGGCCTTGAGGAAGATGGTTGCAGAGGAAGGAGGTGTCCCTGCAGAGGAGGTGATCTTGGT
  TGAACTGTATCCCAATGGATTCCAGCGGTCATTCTTTGATGAAGAGGACCTGAATACCATTGCAGAGGGT
  GACAATGTGTATGCTTTCCAAGTCCCTCCTTCACCTGGTCTGGGGACGCTCTCAGCCCATCCGTCTGGTC
  TGTCAGTGTCACCGCGCTTGCCAGTCCGTGACAGCCAGCGATTCTCTGGGCCTCTCCACAGCGAGAACAG
  GGTGGTCTTTCTCTTCTGTAACCTGGTGGGCTCAGGGCAGCAGGCGAGCAGGTTTGGACCACCGTTCTTG
  ATTCGGGAAGATAGAACAATCTCCTGGGCCCAGCTTCAACAGTGTATTCTCAGCAAGGTTCGATGCCTCA
  TGAGGAGTGAGGTCTCTGCACAGGACCTGGGGACTCTGTTCTCCATCCGGGTTGTGGGGCTCTCCTTGGC
  CTGCAGCTACTTATCTCCAAAGGACAACCGGCCCCTTTGCCACTGGGCAGTTGACAGGGCTTTGCACCTC
  AGGAGGCCAGGAGGCCCTCCTCATGTCAAGCTGGCTGTGGAATGGGACAGCTCTGTCACAGAGCGCCTGT
  TTGGGAGCCTCCAGGAGGAACGGGTCCAGGATGCAGACAGTGTGTGGCGGCAGCAGCAGGCACACCAGCA
  GCCCAGCTGTACCCTGGACGAATGTTTTCAGTCCTATACCAAGGAGGAACAGCTGGCCCAGGATGATGCA
  TGGAAGTGCCCTCACTGCCAAGTCCTCCAGCAGGGTGTGGTCAAGCTGAGTCTGTGGACTCTGCCCGACA
  TCCTGATCATCCATCTGAAACGGTTCTGTCAGGTGGGAGAGAGAAGGAACAAGCTCTCCACGCTGGTGAA
  GTTCCCACTGTCTGGACTCAACATGGCTCCCCATGTAGCACGGAGGAGCACCAACTCCAAGGCGGGGCCC
  GGCCCTTGGTCCTCCTGGAAGCAGCCGATCTGCCTCCCCACCACCTACCCGCTGGACTTCCTCTACGACT
  TGTATGCTGTCTGCAACCACCATGGCAGTCTGCAAGGTGGGCATTACACAGCCTATTGCCGGAACTCCCT
  GGACGGCCAGTGGTACAGCTATGACGACAGCACAGTGGAGGCCCTGCGAGAGGATGAGGTCAACACCAGG
  GGGGCTTACATCCTATTCTACCAGAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAG
  GTTCCACCAGCTCCTCCTTGTCTGATCATTGGCTCATGAGACTCGGGAGCCTCAACAACAGCACAAGGGG
  AAGCTTGCTGTCCTGGAGCTCGGCCCCCTGCCCCTCCATGGCCCGGGTACCCGACTCTCCTGTCTTCACC
  AATGGTGTCTGCCACCAGGACAAGGGAGGAGTTGAGACCAGACCTTTGGTTCGGGGTGTTGGTGGCCGAA
  GTATCAGCATGAAGGCATCTCCTGCTTCCCGATCCAGGCATGGGCCCTTCAAGACCATGCCCCTGAGGTG
  GTCATTTGGACACAGGGAGAAACGTCCCGGGGCATCCGTTGAACTGGTGGAATACCTAGAGTCCAGAAGA
  AGACCCCGTTCTACCAGCCAGTCCATTGTGCCGCTCTTGACTCGTGCTGCTGGTGGGGAAGAGACTTCAG
  CGTCACCGAGGTCAGATGGCACCCTTCCTGCTAAAAGTGAAGACAGCGGTCGAGCCATTGGGCAAGGAAC
  AACTGGGGTGCCCCTTTCCTCATGCCACCTCAACCACCATCCAGCCCTGGGGTCTTTAGATGACAGTCTG
  CACACTGCCAGGACACGAACGGGGAATGTAAGTCAGGATATCAGGCTCCCAAAAAAGTTTGACCTGCCCC
  TCACTGTGATGCCCTCAGTGGGGGATGAAAAGCCAGCTCGCCCTGAGGGCCAGAAGATGACACCTTGGAA
  GGGAAGTAGCCAGGTGGGAAGCCAGAGTAGCCCACCTTCCCCCTCCACTGGCTTGCTTAGAAACTTTAAG
  GACAGTGGCCCAGGTACCTTACCCAAGATGAAGTCTAAGGCTGCCATGGAGGAAAGGGCCCCTGACAAAG
  ACAGGGGACAGGGGACATTCACTCTTTTGAAGTCTGTGTTTTGGAAGAAAGAACACAAGAGGACTGTCAG
  GACTGAGAGCTCACCACCAGCACCCCCAATCTCCCTAGGGAGTGACCGGCTGAGCCCTGCTGCCATGAAT
  GAGCAGGCCCTCAGGATTCGAGAGAGCCCAGCCAAGGGCCTGGGCAACCACATGGAGAGGGACATCAGAT
  CTGCGCCCAGCTCTCTCCACCTCCCCCGAAAAGCCAGCAGGCCCCCTCGAGCCAGTACAGCTGGCACCTC
  ACAAAGGACCATTCCTGGGGAACAGATTTCTTATGGCACCCTGCAGAGAGTGAAATACCACACCCTTTCC
  TTAGGTCGAAAGAAATCCTTACCTGAGTCCAGCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173754
• Insert Size: 3399 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173754.4, NP_776115.2
• RefSeq Size: 4462 bp
• RefSeq ORF: 3399 bp
• Locus ID: 216835
• UniProt ID: Q8BUM9
• Cytogenetics: 11 B3
• Gene Summary: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.