Lats1 (NM_010690) Mouse Untagged Clone

CAT#: MC223684

Lats1 (untagged) - Mouse large tumor suppressor (Lats1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lats1
• Synonyms: AW208599
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223684 representing NM_010690
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGGGTGAAAAGCCAGAAGGGTACAGACAGATGAGGCCTAAGACCTTTCCTGCCAGCAACTACC
  CTGGCAGCAGCCGACAGATGCTGCAGGAGATCCGAGAATCCCTGAGGAATTTATCAAAACCATCTGATGC
  TTCGAAGGCCGAGCATAACCTGAATAAGATGTCGACTGAAGATCCCAGGCAGGTGAGAAATCCACCCAAA
  TTTGGCACACATCATAAAGCCTTGCAAGAAATTCGAAACTCTCTACTACCATTTGCAAATGAAACAAGTT
  CTTCCCGGAGCCCTTCAGAAGTTAATCCACAGATGTTTCAGGATTTGCAGGCTGCTGGCTTTGATGAGGA
  CATGGTTATTCAAGCTCTTCAGAAAACCAATAACAGAAGCATAGAAGCCGCTGTTGAATTCATTAGTAAA
  ATGAGTTACCAAGACCCTCGTCGAGAGCAGATGTCTGCAGCAGCTGCCAGGCCTATTAATGCCACCATGA
  AACCAGGAAATGTGCAACATTCAATTAACCGAAAACAAAGCTGGAAAGGTTCTAAAGAGTCTCTAGTTCC
  TCAGAGACACGGCCCATCTCTAGGAGAAAATGTGGTTTATCGTTCTGAAAGCCCCAACTCACAGGCGGAT
  GTAGGAAGACCTCTGTCTGGATCCGGCATTGCAGCATTTGCTCAAGCTCACCCAAGCAATGGACAGAGAG
  TGAACCCCCCACCACCACCTCAAGTTAGGAGTGTTACTCCTCCACCACCTCCGAGAGGCCAGACCCCACC
  TCCCCGAGGCACCACTCCCCCTCCCCCCTCATGGGAACCAAGCTCTCAGACAAAGCGCTACTCTGGGAAC
  ATGGAGTACGTAATCTCCCGAATCTCCCCTGTTCCACCTGGGGCGTGGCAGGAGGGCTACCCTCCACCAC
  CTCTTACCACTTCTCCCATGAATCCCCCTAGCCAGGCTCAGAGGGCCATTAGTTCTGTTCCAGTTGGTAG
  ACAACCCATCATCATGCAGAGTACTAGCAAATTTAACTTTACACCAGGGCGACCTGGAGTTCAGAATGGT
  GGTGGTCAGTCTGATTTTATCGTGCACCAAAATGTCCCCACTGGTTCTGTGACTCGGCAGCCACCACCTC
  CATATCCTCTGACCCCAGCTAATGGACAAAGCCCCTCTGCTTTACAAACAGGGGCTTCTGCTGCTCCACC
  ATCATTCGCCAATGGAAACGTTCCTCAGTCGATGATGGTGCCCAACAGGAACAGTCATAACATGGAGCTT
  TATAATATTAATGTCCCTGGACTGCAAACAGCCTGGCCCCAGTCGTCTTCTGCTCCTGCGCAGTCATCCC
  CAAGCGGTGGGCATGAAATTCCTACATGGCAACCTAACATACCAGTGAGGTCAAATTCTTTTAATAACCC
  ATTAGGAAGTAGAGCAAGTCACTCTGCTAATTCTCAGCCTTCTGCCACTACAGTCACTGCCATCACACCC
  GCTCCTATTCAACAGCCCGTGAAAAGCATGCGCGTCCTGAAACCAGAGCTGCAGACTGCTTTAGCCCCAA
  CCCATCCTTCTTGGATGCCACAGCCAGTTCAGACTGTTCAGCCTACCCCTTTTTCTGAGGGTACAGCTTC
  AAGTGTGCCTGTCATCCCACCTGTTGCTGAAGCTCCAAGCTATCAAGGTCCACCACCGCCTTATCCAAAA
  CATCTGCTACACCAAAACCCATCTGTCCCTCCATATGAGTCAGTAAGTAAGCCCTGCAAAGATGAACAGC
  CTAGCTTACCCAAGGAAGATGATAGTGAGAAGAGTGCGGACAGTGGTGACTCTGGGGATAAAGAAAAGAA
  ACAGATTACAACTTCACCTATCACTGTTCGGAAAAACAAGAAAGATGAAGAACGAAGAGAGTCTCGGATT
  CAGAGTTACTCCCCACAGGCCTTTAAGTTCTTCATGGAGCAGCACGTAGAGAACGTCCTGAAGTCTCATC
  AGCAGCGTCTGCATCGGAAGAAGCAGCTAGAAAATGAAATGATGCGGGTTGGATTATCTCAAGATGCCCA
  GGATCAAATGAGAAAGATGCTTTGCCAGAAAGAGTCTAACTATATTCGTCTTAAAAGGGCTAAAATGGAC
  AAGTCTATGTTTGTAAAGATAAAGACATTAGGAATAGGAGCGTTTGGTGAAGTCTGTCTAGCAAGAAAAG
  TCGATACTAAAGCTTTGTATGCAACAAAGACTCTTCGAAAGAAAGACGTTCTGCTCCGAAATCAGGTGGC
  TCATGTGAAAGCGGAGAGGGATATCCTAGCAGAAGCCGACAATGAGTGGGTGGTCCGCCTGTACTACTCT
  TTCCAGGACAAGGACAACTTGTACTTTGTGATGGACTACATTCCTGGGGGGGATATGATGAGCCTATTAA
  TTAGAATGGGCATCTTTCCTGAAAATCTGGCACGATTCTACATAGCAGAACTTACCTGTGCAGTTGAAAG
  TGTTCATAAAATGGGTTTTATTCATAGAGATATTAAACCTGATAACATTTTGATTGACCGTGATGGCCAT
  ATTAAATTGACTGACTTTGGCTTGTGCACTGGCTTCAGATGGACACATGACTCCAAGTACTACCAGAGTG
  GGGATCACCCACGGCAAGATAGCATGGATTTCAGTAACGAATGGGGAGATCCTTCCAATTGTCGGTGTGG
  GGACAGACTGAAGCCACTGGAGCGGAGAGCTGCTCGCCAGCACCAGCGATGTCTAGCCCATTCTCTGGTT
  GGGACTCCCAATTATATTGCACCTGAAGTGCTACTGCGAACAGGATATACACAGCTGTGTGACTGGTGGA
  GTGTTGGTGTTATTCTTTTTGAAATGTTGGTGGGACAACCTCCTTTCTTGGCACAAACCCCATTAGAAAC
  ACAAATGAAGGTTATCAACTGGCAAACTTCTCTTCACATCCCTCCTCAAGCTAAGCTGAGTCCTGAAGCC
  TCTGACCTCATTATCAAACTTTGTCGAGGACCAGAAGACCGCCTCGGCAAGAACGGTGCTGATGAGATAA
  AGGCTCATCCATTTTTTAAGACCATCGATTTCTCTAGTGATCTGAGACAGCAGTCTGCTTCATACATCCC
  TAAAATCACGCATCCAACAGATACATCCAATTTCGACCCTGTTGATCCTGATAAATTGTGGAGCGATGGC
  AGCGAGGAGGAAAATATCAGTGACACTCTGAACGGATGGTATAAAAATGGGAAGCACCCCGAGCACGCTT
  TCTATGAGTTCACCTTTCGGAGGTTTTTTGATGACAATGGCTACCCATATAATTATCCAAAGCCTATTGA
  GTATGAATACATTCATTCACAGGGCTCAGAACAACAGTCTGATGAAGATGATCAACACACAAGCTCCGAT
  GGAAACAACCGAGATCTAGTGTATGTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010690
• Insert Size: 3390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010690.1, NP_034820.1
• RefSeq Size: 7222 bp
• RefSeq ORF: 3390 bp
• Locus ID: 16798
• UniProt ID: Q8BYR2
• Cytogenetics: 10 A1
• Gene Summary: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.[UniProtKB/Swiss-Prot Function]