Senp6 (NM_146003) Mouse Untagged Clone

CAT#: MC223670

Senp6 (untagged) - Mouse SUMO/sentrin specific peptidase 6 (Senp6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Senp6
• Synonyms: 2810017C20Rik; E130319N12Rik; mKIAA0797; Susp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223670 representing NM_146003
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGCAAGAGCGGTGGCAGCGCTGGGGCTCTTTTCCTGAAAGCTTTGGATAGATCAGAATCTA
  AGAGGGATGGAGGCTTTAAAAATAATTGGAGCTTTGATCATGAAGAAGAAAGTGAAGGAGATGCAGACAA
  AGATGGGGCAAATCTACTCAGTGTAGAAGATGAGGATTCTGAAATCTCAAAAGGAAAAAAGTTAAATCGG
  AGATCTGAAATTGTTGCTACTAGTTCTGGTGATTTCATCTTGAAGACTTATGTAAGGCGGAGCAAGACTG
  ACGGCTTTAAAACTTTGAAAGGCAACCCAATTGGACTTAACATGTTAAGCAACAATAAGAAACTGAGTGA
  AAGCACCGCAGGCACAGCATTATGTTCTGGAACTGTGGTTCATGGGAGGCGCTTCCATCATGCTCACTCA
  CAGACGCCAGGCATCAGAACAGCGGCTCAGAGGAAAGAATACCCACCTTATGTCCACAAAGCTGAAAACA
  GCCCTGTAATGCTAAGTCATGGCCAAGGTGGAGACCATATAATGAAGAAAACTGAAGAGTCAGAATCATA
  TGTAGAGTCTGAGATTAAGAGGAAAGTCCAGCAGAAGCGGCACTGTAGCACTTACCAGCTTTCTCCTCTG
  TCCCCTGCCTCAAAGAAGTGTTTGACCCATTTAGAGGTTTCTGAACAGCGTGAATATTGCCCAAAATGTG
  GAAAAGAAAAAGAAAATCAGACCAAATGCCAAAGTTGTGGTATTGTTTTTCATAATGATTTGCAAAGAAA
  TTGCCGACAAGCCGTAACTTTGAATGAACCTACTGGACCATTGCTAAGAACATCAATTCATCAGAACTCT
  GGAGGACAGAAATCACAAAACACAGGACTAACAGCTAAGAAATTTTATGGGAACAGTGTGGATAAGATTC
  CAATTGATATTTTGGTCACCTGTGATGATAGCAGACATAATTACATACAGACAAATGGGAAAGTCATTTT
  ACCTGGAGGAAAAATACCTAAACTCACAAACCCAAAAGAGCGAAAGATAAGCGTGTCAGATTTAAACGAT
  CCAATCATTTTGTCTAGTGATGATGATGACGACGACGACGATAGGACTAAGAGAAGAGAGAGCACGTCCC
  CGAAGCCTGCCGACTCAGCATGCTCCTCACCAGTACCATCTACGGGAAAAGTTGAAGCTGCACTAAATGC
  AGATGCCTGTAGAGCAGAGCAGGAACCCAGAAGCAGTCCAGCAGAGCCTGAGTTAAATACCATTGTCATA
  CCACGAAAAGCAAGAATGAAGGACCAGCTTGGTAATTCCATCAGCACACCTTTAAAACGTCGTAAAGTAA
  ACAGTCATGCAGCTTTTATTCACCCTATGTCTTTAAGTTGCCAAAACTTTGAAAGTGTCATTTTAAACTG
  CCGAAGTATACGAGTTGGAACACTCTTCCGACTGTTGGTAGAGCCTGTAATTTTTTCTTTAGAGTCAATC
  ACGATACATCTAGATGGACCAGAAAGTGATCCTGTAGACATTATTTTAAATACCTCTGATCTAACTAAAT
  GTGAATGGTGTAATGTCCGGAAATTACCAGTAGTGTTTCTCCAGGCAATACCAGCAGTTTACCAGAAGCT
  GAGCATGCAGCTACAGATGAGTAAAGAAGATAAAGTTTGGAATGATTGTAAAGGAATAAACAGGATAACA
  AGTTTAGAAGAACAGTACATTATTTTAATTTTTCAAACTGGCCTTGATCATCAGGCAGAGGTGGTCTTTG
  AAAGTATCATTACTGACATTGGTATAAGGAATAATGTTCCCAACTTTTTTGCAAAAATTCTTTTTGACGA
  AGCCAATAGCAGACTTGTTGCCTGTACAAGAAGCTATGAAGAGAGCATCAAAGGAAACTGCGCGCAAAAA
  GAGAACAAAGTGAAAACTGTGTCGTTTGAATCTAAAATACAACTTAGAAGCAAACAAGAATTGCAGTTTT
  TTGATGATGACGAAGAAGCTGGGGAGAGCCATACAATCTTCATTGGCCCTGTAGAAAAATTGATAGTATA
  CCCACCACCTCCAGCTAAGGGAGGCATTTCTGTTACTAATGAGGACCTGCACTGTCTAAGTGAAGGAGAG
  TTTTTAAATGATGTTATTATCGACTTTTATTTGAAATACTTGGTGCTTGAAAAACTGAAGAAAGAAGACG
  CTGACCGAATTCATATATTTAGTTCTTTTTTCTATAAACGCCTTAATCAGAGAGAGAGGAGAAATCCTGA
  GACAACTAATCTGTCGATACAACAAAAACGGCATGGGAGAGTAAAAACGTGGACACGGCATGTAGATATT
  TTTGAGAAGGACTTTATTTTTGTACCCCTTAATGAAGCTGCACATTGGTTTTTGGCTGTTGTTTGTTTCC
  CTGGTTTGGAAAAACCAAAATATGAACCTAATCCTCATTACCATGAAAATGCAGTCATGCAAAAAACTCC
  AAGTGCAGAGGACAGTTGTGTTTCTTCTGCCAGTGAGATGGGTGCTTGTTCACAGAACTCTGCTGCCAAG
  CCTGTGATCAAGAAGATGTTAAATAGAAAACATTGCTTAGCTGTGACTGATTCGAGTGCTGCACAGGAAG
  AAAGTGAGCCTTGTTACCGGAGAAACGCATACAGTGTAAAGTGTAGTATGAAAAAAAAAAATCATGCTAT
  CAATGAAAATGAAGAACCAAGTAACGGAGAATCTACGTGCCAGGACATTTGTGATAGAACCCAAAGTGAG
  AATGGCCTCCGGGATGAATGCTTCAGTTCTGTACATCATCCAGATGCCTTAAGCAAAATCAGACTAAACT
  ACGGTGATCAGTCAGCAGACGGTGGGAAGCTGCTTGAAGATGAGCTCATTGACTTCTCAGAGGATCAGGA
  TGACCCGGATGATAGCAGTGATGACGGACTCCTTGCTGATGAAAACTACAGTTCAGAAATAGGACAGTGG
  CATTTAAAGCCTACTGTCTGTAAACAACCTTGTATCCTCCTTATGGACTCACTCAGAGGCCCTTCTCGTT
  CAAATGTTGTCAAAATTCTAAGAGAGTATTTAGAAGTGGAATGGGAGGTGAAAAAAGGAAGCAAAAGAAG
  TTTTTCCAAAGATGTTATGAAAGGGTCCAACCCAAAAGTCCCACAACAGAACAACTTCAGTGACTGTGGT
  GTCTATGTGCTGCAGTATGTAGAGAGCTTTTTTGAGAATCCAGTACTAAATTTTGAGCTCCCTATGAATT
  TGATGAACTGGTTTCCTCCCCCCAGAATGAAAACAAAAAGAGAAGAAATCCGAAACATAATTTTGAAGCT
  ACAGGAATCACAGAGTAAAGACAAGAAGCTGCTGAAGGACTCCTTAGCAGAGACGTCCTTAGGGGACGGA
  GCAGAGCAGTACGCCAGCGCCAGCGGTGGCTCAGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146003
• Insert Size: 3399 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146003.2, NP_666115.2
• RefSeq Size: 4938 bp
• RefSeq ORF: 3399 bp
• Locus ID: 215351
• UniProt ID: Q6P7W0
• Cytogenetics: 9 E1
• Gene Summary: Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.