Ap4e1 (NM_175550) Mouse Untagged Clone

CAT#: MC223659

Ap4e1 (untagged) - Mouse adaptor-related protein complex AP-4, epsilon 1 (Ap4e1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ap4e1
• Synonyms: 2310033A20Rik; 9930028M04Rik; AV087807
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223659 representing NM_175550
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGACATGGTGGAGAGGACGCTGACCGCACTGCCGGGGCTCTTCCTGCAGAACCAGCTGGGAGGAC
  CTGCGGCCTCCAGAGCACCCTTCTTCTCCAGGCTGGGCGGCCTCATCCGTGGCGTCACTGCACTCTCATC
  CAAGCACGAAGAAGAGAAATTAATCCAGCAGGAACTGAGTAGTCTGAAGGCAACTGTTTCTGCTCCGACC
  ACGACACTGAAAACGATGAAGGAATGTATGGTGAGACTTATATACTGTGAGATGCTTGGATACGATGCTT
  CTTTTGGTTATATACATGCAATCAAGTTGGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTGGGTTATTT
  GGCTGTTTCTTTATTTCTACATGAAAGTCATGAACTTTTGCTTCTCCTTGTGAATACAGTGGTAAAGGAC
  CTGCAGAGCACTAATCTGGTAGAAGTATGTATGGCACTTACTGTTGTCAGCCAGATTTTCCCTCGAGAAA
  TGATTCCAGCCGTTCTTCCACTAATAGAAGATAAACTTCAACATTCTAAGGAGATTATACGAAGAAAAGC
  AGTTCTGGCATTATACAAATTTTACCTCATTGCCCCCAATCAAGTACAGCACATCCACACTAAATTTCGG
  AAAGCGCTTTGTGACAGAGATGTTGGGGTCATGGCTGCCTCTTTGCACATATACCTTAGGATGATTAAGG
  AAAATGCTTCTGGCTATAAAGACTTGACGGAGAGTTTTGTAACAATTTTAAAGCAGGTGGTGGGCGGGAA
  GCTCCCGGTAGAGTTCAGTTACCACAGCGTGCCAGCACCATGGTTACAGATTCAGCTCCTGAGAATACTG
  GGACTGCTGGGGAAAGACGATGAAAGGACAAGTGAATTAATGTATGATGTTCTTGATGAATCCTTACGAA
  GAGCCGAGTTAAATCACAATGTCACTTACGCTATTCTGTTTGAATGTGTACACACAATCTATTCTATTTA
  TCCTAAGTCAGAATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATAAAC
  CTCAAATATTTAGGACTGAAGGCTCTTACCTATGTTATCCAGCAGGATCCTTCTCTGGCTCTTCAACACC
  AGATAACCATAATTGAATGCCTAGACCACCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCTTTA
  CAGAATTACAAATGCACAGAATGTAGTGGTTATTGTACAGAAAATGCTTGAATACTTACATCAGAGCAAA
  GAAGAGCATATCATCATCAGTTTAGTTGGCAGAATTGCTGAGCTGGCTGAGAAATATGCTCCTGACAACG
  TGTGGTTTATTCAGACAATGAATGCTGTGTTTTCAGTGGGAGGAGATGTAATGCATCCAGATATTCTCAG
  CAACTTCCTGAGACTGCTAGCAGAAGGTTTTGATGATGAAACAGAAGATCAACAATTAAGACTCTACGCA
  GTTCAGTCTTATCTTACTTTACTAGATATGGAAAATACTTTCTATCCACAGAGATTTCTTCAAGTTATGA
  GTTGGGTGTTAGGAGAGTACTCCTACCTCTTAGATAAAGAAAGTCCAGAGGAGGTTATAACCAGGCTCTA
  CAAGTTACTTATGAGTGACTCCATTTCTTCAGAAACGAAAGCATGGTTGTTTGCTGCGGTAACCAAGTTG
  ACACCTCAAGCTCATTCTTCTCCCTTAGTTGAGAAATTAATCCAAGAATTCACTGTATCCTTGAATACTT
  GTCTGAGACAGCATGCATTTGAATTGAAACATCTGCATGAAAATACAGAGCTTATGAAGAGCCTGCTTCA
  GGGTGCTCAGAATTGTGAAGACATAGTGGCAGATGCGTCTTTATCTTTTCTGGATGGTTTTGTGGCTGAA
  GGACTCAGTCAGGGAGCAGCACCTTATAAGCCCCATCACCAGCGCCAAGAGGAACAGCTCTCTCAGGAAA
  AGGTTCTCAATTTTGAACCATATGGACTATCATTTTCTTCATCTGGCTTCACTGGACGACAGTCTCCGGC
  TGGCATTTCCCTGGGCTCTGATATATCTGGAAACAGTGCCGAGACAGGGCTGAAAGAGACAAGCAGTTTG
  AAGATGGAAGGGATAAAGAAATTATGGGGTAAAGAGGGCTATCTGCCCAAGAAGGAGAGTGGAACTGGTG
  ATAAACCGGAAGCCTCACATGTTCCTGCAGAGGGTGCAACAGTGGAGAATGTAGACCAAGCCACGACCAG
  AAAGGACCAAGCTCAAGGCCATATCCCTTCTACAGAGGAGAAAGAAAAGCAGCTGTTGGCATCATCGCTG
  TTTGTTGGGCTAGGACCAGAAAATACAGTCGATTTGTTGGGAAAAGCAGATGTTGTGTCTCACAAGTTCA
  GGAGGAAATCAAAACTCAAGGTAGCCCAAAGCGACAAGACACCCAGCGCTCCTACTGCTCCCTGCTCCGC
  CCTCAGCCTTGGCTCAGATGTGGCTGGTGGGGATGAGGACGGTCTGAGTGCTGTGGATAGAGGGGACGGA
  GAACTCAGTTCAGAGCTTTTTCGTTCTGAGTCTCTCTCGGGGCCGCCCTCAGCTGAGAAGCTCGAGAGTG
  TCAGCTTGCCTGTGCCTTCTTTATTTGCTGATAACAACATGGAAGTCTTTAATCCCCCTTCATCCTCTGC
  AACTTCAACTGTCAAGGAAGAAACTCCTGAGTGCAGGCATTCAGGTCTCGTGGAAATCTGCAGTAATGAA
  GCCGTGTCTGTGTCTTCTTACAAAGTCTGGAGGGATGATTGTTTATTGGTGATCTGGGCAGTCACTAGTA
  AAACTGACTCAGAGTTCACAGATGCTCAGTTAGAAATTTTTCCTGTGGAAAATTTCAAGATCATTGAGCA
  ACCTGAATGTTCTTCTCCTGTGATAGAAACAGAACGCACCAAATCCTTTCAATACAGTGTGCAGATGGAA
  AGCCCTTGTATAGAGGGGACTCTCTCTGGTTTTATAAAGTATCAAATGATGGATACGCATTCTGTTCAAC
  TGGAATTTTCCATGAACTTACCATTATTAGATTTTATTAGACCATTAAAGATCTCAACTGAAGACTTTGG
  CAAACTCTGGTTGTCCTTTGCAAATGATGTGAAGCAAACTATAAAAATATCAGAGCCTGGAGTTGCTCTG
  ACTTCTGTCCTAACAGAGCTGCAGCAGAACCTGAGACTTCGTGTGATTGACGTCATAGGCAATGAAGGGC
  TGTTGGCCTGTAAGCTGCTCCCATCCACCCCCTGTGTGCTGCACTGCCGAGTACATGCTGACGCGGTAGC
  TCTGTGGTTCCGGTCCTCTAGCTCTGTTCTTTCAGACTATTTATCGTGTCACTGTCAAAAAGTGATGCAG
  ACATCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175550
• Insert Size: 3369 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175550.3, NP_780759.2
• RefSeq Size: 6524 bp
• RefSeq ORF: 3369 bp
• Locus ID: 108011
• UniProt ID: Q80V94
• Cytogenetics: 2 61.76 cM
• Gene Summary: Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal.[UniProtKB/Swiss-Prot Function]