Sh3pxd2a (NM_008018) Mouse Untagged Clone

CAT#: MC223641

Sh3pxd2a (untagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sh3pxd2a
• Synonyms: 2310014D11Rik; AA589508; AI256723; AI413738; C230050L11; EG329070; Fish; Gm5098; Sh3md1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223641 representing NM_008018
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGCCTACTGCGTGCAAGATGCCACCGTGGTGGACGTGGAGAAGCGGAGGAGCCCCTCTAAACACT
  ATGTATACATTATCAACGTGACCTGGTCTGACTCCACCTCCCAGACTATCTACCGGAGGTACAGCAAGTT
  CTTCGACCTGCAGATGCAGCTTCTGGATAAGTTTCCTATTGAAGGTGGCCAGAAGGATCCGAAGCAAAGG
  ATTATTCCCTTTCTTCCAGGCAAGATCCTCTTCCGGAGAAGCCACATCCGCGACGTGGCTGTGAAGAGAC
  TAAAGCCCATCGATGAATACTGCAGGGCGCTTGTCCGGCTGCCGCCCCACATTTCACAGTGTGACGAAGT
  CTTCCGGTTCTTTGAGGCACGGCCTGAGGATGTCAACCCTCCAAAAGAAGACTATGGCAGTTCCAAGAGG
  AAATCAGTGTGGTTGTCCAGCTGGGCTGAGTCTCCCAAGAAGGACGTGACAGGTGCCGACACCAACGCCG
  AGCCCATGATCCTGGAACAGTACGTGGTGGTGTCCAACTATAAGAAACAAGAGAACTCGGAGCTGAGCCT
  CCAGGCCGGGGAGGTGGTAGATGTCATCGAGAAGAACGAAAGCGGCTGGTGGTTTGTGAGCACATCTGAA
  GAGCAAGGTTGGGTCCCCGCCACCTACTTGGAGGCCCAGAATGGCACACGAGACGACTCGGACATCAACA
  CCTCCAAGACTGGGGAAGTGTCCAAGAGACGCAAGGCACACCTGCGGCGCCTGGATCGCCGGTGGACCCT
  GGGCGGGATGGTCAACAGGCAGCACAGCCGAGAAGAGAAGTATGTCACTGTGCAGCCCTACACCAGCCAG
  AGCAAAGACGAGATCGGCTTCGAGAAGGGTGTCACCGTGGAGGTGATTCGAAAGAACCTGGAAGGCTGGT
  GGTACATCAGGTACCTCGGCAAAGAGGGTTGGGCACCAGCGTCCTACCTGAAGAAGGCCAAGGATGACCT
  GCCGACCCGGAAGAAGAACCTGGCGGGTCCGGTGGAGATCATAGGGAACATTATGGAGATCAGCAACCTT
  CTCAACAAGAAGGCATCTGGGGATAAGGAGGCTCCGGCTGAAGGCGAGGGATCCGAGGCCCCCATCACCA
  AGAAAGAGATCAGCTTACCGATCCTCTGCAACGCCTCCAATGGCAGCGCCTTGGCCATTCCCGAGAGGAC
  CACATCCAAGCTAGCCCAGGGCTCCCCAGCTGTGGCCAGGATCGCCCCTCAGAGGGCCCAGATCAGCTCC
  CCAAATCTGAGGACAAGACCTCCCCCGCGCAGAGAATCCAGCCTGGGGTTCCAGCTGCCAAAGCCGCCAG
  AGCCCCCTTCTGTTGAGGTAGAATACTACACCATTGCTGAATTCCAGTCCTGCATTTCTGACGGGATCAG
  CTTTCGAGGCGGACAGAAGGCAGAGGTCATCGACAAGAACTCGGGTGGTTGGTGGTACGTGCAGATCGGG
  GAGAAGGAGGGCTGGGCCCCAGCCTCATACATTGACAAGCGCAAGAAACCCAACCTCAGCCGCCGAACCA
  GCACTCTGACGCGGCCCAAGGTGCCGCCACCTGCGCCCCCCAGCAAGCCTAAGGAGGCCGAGGAGAATCC
  TGTGGGTGCCTGTGAGAGCCAGGGCTCCCCACTGAAGGTTAAATACGAGGAACCCGAGTATGACGTCCCT
  GCCTTTGGCTTTGACTCAGAGCCCGAGATGAATGAAGAGCCTTCAGGGGACAGAGGTTCAGGTGACAAGC
  ATCCCGCCCAGCCCCGAAGGATCTCGCCTGCCTCTTCCCTGCAACGGGCCCATTTCAAGGTGGGTGAGTC
  TTCTGAGGACGTGGCCCTGGAAGAGGAGACCATCTATGAGAATGAGGGCTTCAGGCCATACACAGAAGAC
  ACCCTGTCTGCCAGAGGCTCCTCTGGGGACAGTGACTCCCCTGGGAGCTCCTCTTTGTCCCTTGCCGTGA
  AAAACTCCCCTAAATCAGATTCCCCCAAATCCTCATCACTCCTAAAGCTCAAAGCAGAGAAGAATGCCCA
  GGCAGAACTGGGGAAAAACCAGTCCAACATCTCCTTCTCCTCCTCTGTCACCATCAGCACCACCTGTTCC
  TCCTCCTCATCCTCGTCCTCCTTGTCCAAGAACAATGGTGACCTGAAACCACGTTCTGCCTCAGATGCAG
  GTATCCGTGACACCCCTAAGGTTGGGACCAAGAAAGATCCTGATGTGAAGGCCGGGCTGGCCTCCTGCGC
  CCGAGCCAAGCCATCCGTGAGACCAAAGCCAGTCCTGAACCGAGCGGAGTCTCAAAGCCAGGAGAAGATG
  GATATTAGTTCCTTACGGCGCCAGCTGAGGCCCACAGGCCAGCTCCGGGGGGGCCTCAAGGGCTCTAGGA
  GTGAGGACTCAGAGCTGCCTCCACAGATGGCTTCTGAGGGATCCAGGCGAGGTTCTGCGGACATCATCCC
  TCTCACGGCCACCACTCCCCCGTGTGTCCCCAAAAAGGAATGGGAAGGGCAAGGCGCCACCTACGTGACG
  TGCAGCGCCTATCAGAAGGTCCAGGACTCGGAGATCAGCTTCCCCGAAGGCGCCGAGGTGCACGTGCTGG
  AGAAGGCGGAAAGTGGGTGGTGGTACGTGAGGTTTGGGGAGCTGGAGGGCTGGGCTCCTTCCCACTACTT
  GGTGGCCGAGGAGAACCAGCAACCTGACACAGCTAGCAAAGAGGGAGACACAGGAAAGAGCTCGCAGAAC
  GAGGGCAAGTCAGACAGCCTGGAAAAGATTGAGAAGCGTGTGCAGGCGCTCAACACTGTGAACCAGAGCA
  AGAGGGCCACCCCACCCATCCCCTCGAAGCCTCCCGGGGGCTTCGGCAAGACCTCGGGCACCGTAGCGGT
  GAAGATGAGGAACGGGGTCCGGCAAGTGGCCGTCAGGCCCCAATCTGTGTTTGTGTCTCCGCCACCCAAG
  GACAACAACCTGTCCTGTGCCCTTCGGAGGAACGAGTCGCTAACGGCCACCGACAGCCTCAGAGGTGTCC
  GCAGGAACTCCTCCTTTAGCACCGCACGGTCAGCAGCCGCTGAGGCCAAGGGCCGCCTGGCCGAGCGGGC
  TGCCAGCCAGGGCTCAGAATCGCCCCTGCTGCCTACCCAGCGCAAAGGCATCCCTGTCTCCCCCGTGCGT
  CCCAAGCCCATAGAGAAGTCCCAGTTTATCCACAACAACCTCAAGGATGTGTACATCTCGATTGCAGACT
  ATGAGGGGGACGAAGAGACGGCTGGCTTCCAGGAGGGGGTGTCCATGGAGGTGCTGGAGAAGAACCCCAA
  TGGCTGGTGGTACTGCCAGATCCTGGATGAGGTGAAGCCCTTCAAGGGCTGGGTACCCTCCAACTACCTT
  GAGAAGAAGAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008018
• Insert Size: 3375 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008018.4, NP_032044.2
• RefSeq Size: 10464 bp
• RefSeq ORF: 3375 bp
• Locus ID: 14218
• UniProt ID: O89032
• Cytogenetics: 19 C3
• Gene Summary: Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.