Dsg2 (NM_007883) Mouse Untagged Clone

CAT#: MC223640

Dsg2 (untagged) - Mouse desmoglein 2 (Dsg2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dsg2
• Synonyms: AA408168; D18Ertd293; D18Ertd293e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223640 representing NM_007883
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGAGCCCGGGTGACCGGTGCGCCCTGCTGCTGCTGGTGCAGCTGCTGGCGGTGGTCTGCTTGG
  ACTTTGGAAACGGACTTCACTTAGAGGTCTTCAGCCCAAGAAATGAAGGCAAACCGTTCCCTAAGCACAC
  TCACTTGGTTCGTCAAAAGAGGGCCTGGATCACTGCCCCTGTGGCTCTGCGGGAGGGCGAAGACCTGTCC
  AGAAAGAACCCGATTGCCAAGATACACTCTGACCTTGCAGAAGAAAAAGGGATAAAAATCACGTACAAGT
  ACACTGGGAAGGGAATTACAGAACCGCCTTTCGGCATATTCGTCTTTGATAGAAACACAGGAGAACTGAA
  CATCACTAGCATTCTTGACCGGGAAGAAACACCATATTTTCTGCTGACAGGCTATGCATTGGACTCCAGA
  GGAAACAACCTGGAAAAGCCCTTGGAACTACGCATCAAAGTTCTGGACATCAATGACAACGAGCCAGTGT
  TCACACAGGAGGTCTTTGTTGGGTCCATTGAGGAATTGAGTGCAGCACATACACTTGTGATGAAAATCAC
  CGCCACAGATGCAGATGACCCGGAGACTCTGAATGCTAAAGTCTCCTACAGAATTGTCTCTCAGGAGCCT
  GCAAATAGTCATATGTTCTACCTAAATAAAGACACGGGGGAGATCTATACGACCAGTTTTACTTTGGACA
  GAGAGGAACACAGCAGCTATTCCTTGACGGTGGAAGCAAGAGATGGTAACGGGCAGATAACAGACAAGCC
  AGTCCAGCAAGCTCAAGTTCAGATCCGTATATTGGATGTCAATGACAATATACCTGTGGTAGAAAACAAA
  ATGTATGAGGGGACAGTGGAAGAAAACCAGGTCAATGTAGAAGTCATGCGGATCAAAGTGACCGATGCAG
  ATGAAGTGGGCTCTGATAACTGGCTAGCAAACTTTACATTTGCATCAGGAAATGAAGGGGGCTATTTCCA
  CATTGAGACTGACACACAGACTAATGAAGGGATTGTGACCCTTGTCAAGGAAGTGGACTATGAAGAAATG
  AAGAAGCTAGACTTGAGCATCATTGTCACTAACAAAGCAGCTTTCCACAAGTCCATTCTGAGCAAGTACA
  AGGCCACGCCCATTCCCATCACTGTCAAGGTCAAGAACGTGGTTGAAGGCATTCATTTCAAGAGCAGCGT
  AGTCTCTTTCCGAGCTAGTGAGGCAATGGATAGATCCAGCCTCAGCAGGTCGATTGGAAATTTTCAAGTT
  TTTGATGAAGACACTGGTCAAGCAGCTAAAGTAACATATGTAAAAGTGCAAGACACTGACAACTGGGTCT
  CTGTGGACTCCGTCACTTCAGAGATTAAGCTTGTAAAGATTCCTGACTTTGAATCTAGATATGTCCAAAA
  TGGTACCTACACTGCAAAGGTTGTGGCCATATCCAAAGAACATCCTCAAAAAACCATCACTGGCACCATC
  GTTATCACTGTTGAAGACGTCAATGACAATTGTCCCGTGCTGGTGGACTCTGTACGGAGTGTCTGTGAGG
  ATGAACCATATGTGAATGTCACTGCAGAGGATTTGGATGGGGCCCAGAACAGTGCGCCATTCAGCTTCTC
  CATCATTGACCAGCCTCCTGGAACGGCACAGAAGTGGAAAATAACGCACCAGGAAAGTACCAGTGTGCTG
  CTGCAGCAGAGCGAGCGGAAACGCGGGAGAAGTGAGATTCCCTTCCTCATTTCCGACAGCCAGGGCTTCA
  GCTGCCCCGAAAGGCAGGTCCTTCAGCTCACTGTATGCGAGTGTCTGAAGGGCGGTGGCTGTGTGGCTGC
  ACAGTATGACAACTACGTCGGGTTGGGCCCTGCCGCCATCGCTCTCATGATTCTAGCACTCCTGCTCCTG
  CTCCTGGTGCCGCTCTTGCTGTTGATATGCCACTGTGGAGGGGGCGCCAAAGGCTTCACCCCCATTCCTG
  GGACAATAGAGATGCTGCACCCTTGGAATAATGAAGGGGCACCTCCTGAGGACAAGGTGGTGCCATCGCT
  TCTGGTGGCCGATCATGCAGAGAGCTCGGCAGTGAGAGGCGGCGTAGGAGGTGCGATGCTCAAGGAAGGC
  ATGATGAAAGGCAGCAGCTCAGCTTCCGTTACCAAAGGGCAGCATGAGCTGTCTGAGGTTGACGGAAGAT
  GGGAAGAACACAGAAGCCTCCTCACCGCTGGGGCCACTCACCATGTAAGGACAGCAGGAACCATCGCTGC
  CAACGAAGCCGTAAGGACAAGAGCCACGGGGTCTTCCAGAGACATGAGTGGGGCTCGAGGAGCCGTTGCC
  GTGAATGAGGAATTCTTAAGAAGTTACTTCACAGAGAAAGCGGCCTCCTACAATGGGGAAGACGACCTTC
  ACATGGCCAAAGACTGCCTTCTCGTTTACTCTCAGGAAGACACGGCCTCCCTCCGAGGCTCGGTCGGGTG
  CTGCAGTTTCATCGAGGGAGAACTCGATGACCTGTTCCTGGATGATCTTGGCCTTAAATTCAAGACCCTA
  GCTGAAGTTTGCCTAGGTCGAAAGATCGATCTGGATGTGGACATTGAACAGAGGCAGAAGCCGGTCAGAG
  AAGCGAGCGTGAGTGCAGCTTCTGGCTCGCACTATGAGCAAGCGGTAACCAGCTCAGAGAGCGCGTACTC
  CTCTAACACCGGCTTCCCCGCCCCCAAACCTCTGCACGAAGTGCACACAGAGAAAGTCACACAGGAAATC
  GTCACTGAGAGCTCTGTATCTTCCAGGCAGAGTCAGAAGGTAGTACCGCCACCTGATCCTGTGGCTTCTG
  GTAATATTATAGTGACGGAAACTTCCTATGCCAAAGGCTCAGCAGTGCCACCCAGCACTGTGCTCCTGGC
  TCCCAGACAGCCACAGAGCCTGATCGTGACAGAGAGGGTGTATGCTCCAACCTCCACCTTGGTGGATCAG
  CATTATGCCAATGAAGAAAAAGTCCTTGTTACCGAACGAGTGATCCAGCCTAATGGGGGCATCCCTAAGC
  CCCTTGAGGTCACCCAGCATCTGAAAGATGCACAGTATGTAATGGTGAGGGAAAGAGAGAGCATCCTTGC
  TCCCAGCTCAGGCGTGCAGCCCACTCTGGCAATGCCCAGCGTGGCAGCAGGAGGACAGAATGTCACCGTG
  ACAGAAAGAATACTAACTCCTGCTTCCACTCTGCAGTCCAGCTACCAGATTCCCAGTGAAACCTCCATCA
  CGGCTAGGAACACTGTGCTCTCTAGTGTGGGAAGCATAGGTCCTCTGCCCAATTTAGATCTAGAGGAATC
  TGATCGTCCCAATTCTACTATAACCACATCTTCCACCAGGGTCACCAAGCATAGCACCATGCAACATTCT
  TACTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007883
• Insert Size: 3369 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007883.3, NP_031909.2
• RefSeq Size: 5730 bp
• RefSeq ORF: 3369 bp
• Locus ID: 13511
• UniProt ID: O55111
• Cytogenetics: 18 11.42 cM
• Gene Summary: This gene encodes a member of the cadherin family of proteins that forms an integral transmembrane component of desmosomes, the multiprotein complexes involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Mice lacking the encoded protein die in utero. Mutant mice lacking a part of the extracellular adhesive domain of the encoded protein develop cardiac fibrosis and dilation. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. [provided by RefSeq, Jan 2016]