Stxbp5 (NM_001081344) Mouse Untagged Clone

CAT#: MC223621

Stxbp5 (untagged) - Mouse syntaxin binding protein 5 (tomosyn) (Stxbp5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stxbp5
• Synonyms: 0710001E20Rik; 4930565N16Rik; AW742610; LGL3; mKIAA1006
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223621 representing NM_001081344
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAATTCAACATCAGGAAGGTGCTGGACGGCCTGACCGCAGGCTCGTCCTCGGCCTCGCAACAGC
  AGCAACAGCAGCAGCACCCGCCTGGGAACCGGGAGCCGGAGATCCAGGAGACGCTCCAGTCCGAACACTT
  CCAACTCTGCAAGACTGTTCGCCATGGATTCCCCTATCAGCCCTCAGCCCTGGCCTTTGATCCCGTTCAG
  AAGATCCTGGCGGTAGGAACTCAGACTGGTGCTTTAAGGCTCTTTGGTCGTCCAGGAGTGGAATGTTATT
  GCCAGCACGACAGCGGAGCAGCAGTGATCCAACTCCAGTTCCTGATTAATGAGGGAGCCCTTGTGAGTGC
  CTTGGCTGATGACACCCTACACTTATGGAATTTACGTCAAAAAAGGCCTGCCGTACTACATTCACTTAAA
  TTTTGCAGAGAAAGGGTTACATTTTGCCATCTGCCTTTCCAGAGTAAGTGGCTCTATGTGGGCACTGAGC
  GAGGAAACATACACATTGTTAATGTGGAGTCCTTCACACTCTCAGGCTATGTCATTATGTGGAATAAAGC
  CATCGAACTGTCATCTAAAGCTCACCCAGGACCAGTTGTGCATATAAGTGACAATCCCATGGACGAGGGG
  AAGCTTTTGATTGGCTTCGAATCTGGAACAGTAGTCTTATGGGACCTCAAGTCAAAGAAGGCTGACTACA
  GATACACATACGACGAGGCTATTCACTCTGTGGCTTGGCATCATGAAGGAAAACAATTTATTTGCAGTCA
  TTCTGACGGTACTTTGACCATATGGAATGTGAGATCCCCTGCTAAACCTGTACAGACCATCACTCCTCAC
  GGAAAACAGTTGAAGGATGGGAAGAAGCCAGAGCCATGCAAGCCTATCCTGAAGGTGGAGTTGAAGACAA
  CAAGATCCGGGGAACCTTTTATTATTTTGTCAGGAGGCTTATCCTATGATACCGTGGGAAGAAGACCCTG
  CTTAACAGTGATGCACGGGAAAAGCACAGCAGTGCTGGAAATGGACTATTCAATCGTTGACTTTCTCACA
  CTCTGTGAAACGCCATATCCAAACGATTTTCAGGAACCATATGCTGTGGTTGTTCTCCTGGAAAAGGACT
  TAGTGCTGATAGACCTTGCACAAAATGGATACCCTATATTTGAGAATCCCTACCCTTTGAGTATACACGA
  GTCCCCTGTTACATGTTGTGAATATTTTGCTGATTGTCCTGTGGATCTTATTCCTGCACTTTACTCTGTT
  GGAGCTAGACAGAAGCGTCAAGGTTACAGCAAAAAGGAATGGCCCATCAATGGTGGTAATTGGGGCTTGG
  GTGCTCAAAGTTACCCAGAAATAATTATTACAGGGCATGCTGATGGCTCGGTGAAATTCTGGGACGCTTC
  TGCAATAACTCTACAAGTACTATATAAATTAAAAACATCTAAAGTATTTGAGAAGTCAAGAAACAAAGAT
  GACAGACAGAACACAGACATTGTAGATGAAGATCCATATGCCATTCAGATCATCTCCTGGTGCCCAGAGA
  GCAGGATGCTGTGCATAGCTGGAGTGTCAGCCCATGTCATCATCTATAGATTCAGCAAGCAGGAAGTGCT
  TACCGAAGTCATCCCGATGCTTGAAGTTCGACTGTTATATGAAATAAATGATGTGGATACTCCAGAGGGT
  GAGCAGCCCCCGCCTCTGTCCACCCCCGTGGGCAGCTCCAACCCTCAGCCCATTCCTCCTCAGTCTCATC
  CGTCTACCAGCAGCAGCTCATCTGACGGGCTTCGTGATAACGTTCCTTGCTTAAAAGTTAAAAACTCCCC
  ACTTAAGCAGTCTCCAGGCTATCAGACAGAGCTGGTCATTCAGTTGGTGTGGGTGGGTGGAGAACCCCCG
  CAGCAGATCACCAGCCTGGCACTCAACTCTTCCTATGGACTGGTGGTTTTCGGCAACTGTAACGGCATCG
  CAATGGTTGACTACCTCCAGAAAGCAGTACTGCTCAACCTCAGCACCATCGAACTTTACGGCTCAAATGA
  TCCTTATCGGAGAGAACCTCGCTCGCCCCGAAAATCCCGACAGCCTTCAGGAGCGGGCCTGTGTGATATC
  ACCGAAGGGACTGTCGTCCCGGAGGATCGCTGCAAATCTCCCACTTCCGCAAAGATGTCAAGGAAATTAA
  GCTTGCCAACTGACCTAAAGCCTGATTTAGATGTAAAAGACAATTCCTTCAGCAGATCGCGGAGTTCAAG
  TGTAACCAGCATTGACAAAGAGTCCCGGGAAACCATCTCTGCTCTTCATTTCTGTGAGACTTTGACGCGG
  AAGGCCGACTCTTCCCCCTCCCCCTGCCTGTGGGTCGGAACTACAGTGGGAACTGCGTTTGTCATCACGC
  TGAATCTCCCCCCGGGGCCTGAGCAGAGACTCCTTCAGCCAGTGATTGTGTCTCCAAGCGGTACTATATT
  GAGGTTAAAAGGTGCAATCTTGAGGATGGCATTTCTGGATGCCACGGGCTGCTTAATGTCTCCTGCATAC
  GAACCCTGGAAAGAACACAACGTTGCTGAAGAAAAAGACGAAAAGGAGAAATTGAAAAAGCGGCGACCTG
  TCTCAGTGTCCCCCTCCTCTTCTCAGGAAATTAGTGAGAACCAGTACGCAGTGATATGCTCTGAAAAGCA
  AGCAAAGGTCATGTCACTGCCAACCCAGAGCTGTGCATACAAGCAGAACATCACCGAGACGTCCTTCGTG
  CTCCGCGGAGACATTGTCGCACTGAGTAACAGTGTCTGCCTCGCCTGCTTCTGTGCCAATGGCCATATCA
  TGACTTTCAGTTTGCCAAGCTTGAGGCCTCTCCTGGATGTCTACTACCTGCCCCTTACCAACATGCGGAT
  AGCCAGGACATTCTGCTTCGCCAACAACGGACAAGCCTTATACCTTGTTTCACCTACAGAAATCCAGAGA
  CTCACCTACAGTCAGGAGACATGTGAAAATCTTCAGGAGATGCTCGGTGAACTCTTCACGCCTGTAGAGA
  CACCAGAAGCACCAAACAGAGGCTTCTTCAAAGGCTTATTTGGAGGCGGTGCACAATCTCTCGATAGAGA
  AGAACTATTTGGAGAGTCGTCCTCGGGAAAGGCCTCCAGGAGCCTCGCACAACACATCCCGGGCCCTGGT
  GGGATCGAAGGTGTGAAAGGAGCCGCGTCAGGAGTGGTGGGTGAACTTGCCCGAGCCAGGCTGGCCCTCG
  ACGAAAGAGGACAGAAGCTCAGTGACTTGGAAGAAAGGACGGCAGCCATGATGTCCAGCGCAGACTCCTT
  CTCCAAACATGCTCATGAGATGATGCTGAAATACAAAGATAAGAAGTGGTACCAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081344
• Insert Size: 3351 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081344.2, NP_001074813.2
• RefSeq Size: 9074 bp
• RefSeq ORF: 3351 bp
• Locus ID: 78808
• Cytogenetics: 10 A1
• Gene Summary: Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release (By similarity). Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane.[UniProtKB/Swiss-Prot Function]