Adamts10 (NM_172619) Mouse Untagged Clone

CAT#: MC223599

Adamts10 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 10 (Adamts10), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adamts10
• Synonyms: 9430006O18; Adam-ts10; Adamts-10; AW045948; Zn; Znmp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223599 representing NM_172619
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGCCTGCCAGATCCTCCGCTGGGCCCTTGCCCTGGGGCTGGGCCTCACATTCAAGGTCACGC
  ATGCCTTCAGATCTCAAGATGAGCTCCTGTCCAGTTTGGAGAGCTATGAGATTGCCTTCCCAACTCGAGT
  GGACCACAACGGGGCAATGCTGGCCTTCTCTCCACCTGCCTTCCGGAGGCAGCGTCGGGGTGCAGGGGCT
  ACAACTGAGTCCCGCCTATTCTACAAGGTGGCCGCACCCAGCACTCACTTCCTGCTGAACCTGACCCGCA
  GCCCCCGTCTCCTGGCAGGGCACGTCTCGGTGGAATACTGGACACGGGAAGGCCTGGCTTGGCAGAGGGC
  TGCCCGGGCCCACTGCCTATACGCTGGCCACCTGCAGGGCCAGGCTGGTAGCTCCCATGTGGCCGTCAGC
  ACCTGTGGGGGCCTGCATGGCCTGATTGTGGCAGATGATGAAGAGTATCTGATTGAACCCCTGCAAGGTG
  GACCCAAAGGTCACCGTGGCCCAGAAGAGAGTGGCCCCCATGTAGTATACAAGCGTTCCTCTCTGCGTCA
  CCCCCATCTGGACACAGCCTGTGGAGTGAGAGATGAGAAACCGTGGAAGGGTCGTCCATGGTGGTTACGT
  ACCCTGAAGCCACCACCTGCCAGGCCCCTGGGGAATGAATCTGAGCGAGGCCAGCTGGGCCTGAAGAGAT
  CAGTCAGCAGAGAGCGCTATGTGGAGACCCTGGTGGTAGCCGACAAGATGATGGTGGCCTACCATGGGCG
  GAGAGATGTGGAGCAGTATGTGTTGGCCATCATGAACATTGTTGCCAAACTTTTCCAGGACTCGAGTCTG
  GGAAACATCGTCAACATCCTGGTCACTCGCCTCATCCTGCTCACAGAGGACCAGCCCACCCTGGAGATCA
  CCCACCATGCCGGGAAGTCACTGGACAGCTTCTGTAAGTGGCAGAAATCCATCGTGAGCCACAGTGGCCA
  TGGCAACGCCATCCCAGAGAATGGTGTGGCAAACCATGACACAGCTGTGCTCATCACACGCTATGACATC
  TGCATCTACAAGAACAAACCCTGCGGCACTCTAGGCCTGGCCCCTGTGGGTGGAATGTGTGAGCGTGAGA
  GGAGCTGCAGTATCAATGAAGATATCGGCCTGGCCACGGCTTTCACCATTGCCCATGAGATCGGGCACAC
  ATTTGGCATGAATCACGATGGCGTGGGAAACGGCTGTGGGGCCCGTGGGCAGGACCCAGCGAAGCTCATG
  GCTGCCCACATTACCATGAAGACTAATCCATTCGTGTGGTCATCGTGTAGTCGAGACTACATCACCAGCT
  TTCTGGACTCGGGCCTGGGGCTTTGCTTGAATAACCGGCCTCCTAGACAGGACTTCGTGTACCCAACGGT
  GGCTCCCGGCCAGGCCTATGATGCTGATGAGCAGTGCCGATTCCAGCATGGAGTCAAATCGCGTCAGTGT
  AAATACGGGGAGGTCTGCAGTGAACTGTGGTGTCTGAGCAAGAGCAATCGGTGCATCACCAATAGCATCC
  CAGCTGCTGAGGGAACACTGTGCCAAACACACACTATCGACAAAGGGTGGTGCTACAAACGAGTCTGTGT
  CCCCTTTGGCTCTCGGCCAGAGGGTGTAGACGGGGCCTGGGGCCCTTGGACTCCATGGGGTGACTGCAGC
  AGGTCATGTGGCGGTGGTGTGTCATCTTCTAGCCGTCACTGCGACAGTCCCAGGCCAACCATTGGGGGCA
  AGTACTGTCTGGGCGAGAGACGAAGGCACCGGTCCTGCAACACCAATGACTGTCCACCTGGCTCCCAGGA
  CTTCAGAGAAATGCAGTGCTCTGAATTTGACAGTGTCCCTTTCCGCGGAAAATTCTACACGTGGAAGACA
  TACCGAGGAGGGGGTGTGAAGGCCTGTTCGCTGACTTGCCTAGCAGAAGGCTTCAACTTTTATACGGAGC
  GAGCAGCAGCTGTGGTGGATGGAACACCCTGCCGCCCTGACACGGTGGACATTTGTGTCAGCGGCGAGTG
  CAAGCATGTAGGCTGTGACAGGGTCCTGGGTTCTGATCTCCGAGAGGACAAATGCCGTGTGTGTGGGGGT
  GATGGCAGTGCCTGTGAGACCATTGAAGGTGTCTTTAGCCCAGCTTTGCCAGGAACTGGGTATGAGGACG
  TCGTCTGGATCCCCAAAGGCTCGGTCCACATTTTCATCCAAGATCTGAACCTGTCCCTGAGTCACCTGGC
  CCTAAAGGGGGACCAAGAGTCTCTGCTACTGGAGGGGCTACCTGGGACCCCCCAACCTCACCGCCTTCCC
  CTGGCTGGAACCACATTTCATCTACGGCAGGGGCCGGACCAGGCACAGAGCCTGGAAGCCCTGGGACCCA
  TTAATGCATCTCTCATCATCATGGTGCTGGCCCAGGCAGAGTTGCCTGCTCTCCACTACCGCTTCAATGC
  ACCCATTGCCCGGGATGCACTGCCTCCCTACTCCTGGCACTATGCCCCCTGGACCAAATGCTCAGCCCAG
  TGTGCAGGCGGCAGCCAGGTCCAAGTAGTGGAGTGCCGAAATCAGCTGGACAGCTCAGCAGTGGCCCCAC
  ACTACTGTAGTGGCCACAGTAAATTGCCCAAGAGGCAGCGTGCCTGCAACACAGAACCATGTCCACCAGA
  TTGGGTTGTAGGAAACTGGTCACGCTGCAGCCGTAGCTGTGACGCTGGTGTGCGTAGCCGCTCAGTGGTG
  TGCCAACGCCGGGTGTCTGCTGCAGAGGAAAAAGCCTTAGACGACAGTGCCTGTCCACAGCCACGCCCAC
  CTGTGCTGGAGGCCTGCCAAGGCCCAATGTGCCCTCCTGAGTGGGCAACCCTCGACTGGTCTGAGTGTAC
  CCCAAGCTGTGGGCCTGGTCTCCGCCACCGAGTGGTCCTTTGTAAGAGTGCAGATCAACGATCTACTCTG
  CCCCCTGGGCACTGCCTTCCTGCAGCCAAGCCACCATCTACTATGCGATGTAACTTGCGCCGCTGCCCTC
  CTGCCCGCTGGGTGACCAGTGAGTGGGGTGAGTGTTCCACACAGTGTGGCCTCGGCCAGCAGCAGCGCAC
  AGTGCGCTGCACCAGCCACACCGGCCAGCCATCTCGAGAGTGCACTGAAGCCTTGCGGCCATCCACCATG
  CAGCAGTGTGAGGCCAAGTGTGACAGTGTGGTGCCGCCTGGAGATGGCCCAGAAGAATGCAAGGATGTGA
  ACAAGGTGGCTTACTGCCCCCTGGTGCTCAAATTTCAGTTCTGTAGCCGAGCCTACTTCCGCCAGATGTG
  CTGCAAAACCTGCCAAGGCCGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172619
• Insert Size: 3315 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172619.4, NP_766207.2
• RefSeq Size: 4176 bp
• RefSeq ORF: 3315 bp
• Locus ID: 224697
• UniProt ID: P58459
• Cytogenetics: 17 B1
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate a functional, zinc-dependent metallopeptidase enzyme. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation lacks publicly available full-length transcript support in mouse. However, it is supported by the full-length human transcript AF163762.1, by partial mouse EST alignments including BB840099.1, and by the full-length sequence described for this mouse protein in PMID:15355968. Other mouse full-length transcript variants are available for this gene, including BC082773.1, AK042525.1 and BC056427.1, but it should be noted that all of these include an alternative splice acceptor site in exon 6, which results in a premature termination codon that likely leads to nonsense-mediated mRNA decay (NMD). The exon combination represented in this CCDS is therefore more likely to be protein-coding.