Smarcc1 (NM_009211) Mouse Untagged Clone

CAT#: MC223589

Smarcc1 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 (Smarcc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smarcc1
• Synonyms: AI115498; BAF155; msp3; Rsc8; SRG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223589 representing NM_009211
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGACAGCGGGTGGCGGTCCGGGAGCAGCAGCAGGCGCCGTGGGTGCAGGGGGTGCGGCGGCGG
  CCTCCGGGCTGGCCGTGTACCGGAGGAAGGACGGGGGCCCGGCCAGCAAGTTTTGGGAGAGCCCGGACAC
  GGTGTCCCAGCTAGATTCGGTGCGAGTCTGGCTGGGCAAGCACTACAAGAAGTATGTTCATGCAGATGCT
  CCTACCAATAAAACACTAGCTGGACTGGTGGTGCAGCTTCTACAGTTCCAAGAAGATGCCTTTGGGAAGC
  ATGTCACCAACCCAGCTTTCACCAAACTACCTGCAAAATGTTTCATGGATTTCAAAGCTGGAGGCACCTT
  GTGTCACATTCTTGGGGCAGCTTACAAGTACAAAAATGAACAGGGCTGGCGGAGATTTGATCTTCAGAAC
  CCATCCCGAATGGATCGTAACGTTGAAATGTTCATGAACATTGAGAAAACATTGGTACAGAACAACTGTC
  TGACTAGACCAAACATCTACCTCATTCCAGACATTGATTTGAAGTTGGCTAACAAGTTGAAAGATATCAT
  CAAACGGCATCAGGGGACATTTACTGATGAGAAGTCAAAAGCTTCCCACCATATTTATCCATATCCTTCC
  TCACAAGAGGATGAGGAGTGGCTGAGACCAGTGATGAGGAGAGACAAGCAGGTGCTGGTGCACTGGGGTT
  TCTACCCAGACAGCTATGACACTTGGGTCCACAGTAATGATGTTGATGCTGAAATTGAAGATGCACCAAT
  CCCAGAAAAGCCCTGGAAGGTTCATGTAAAATGGATTTTGGACACTGACGTTTTCAATGAATGGATGAAT
  GAAGAGGATTATGAAGTGGATGAGAACAGAAAGCCAGTGAGCTTTCGTCAACGAATTTCAACAAAGAATG
  AAGAGCCAGTCAGAAGTCCAGAAAGGAGAGACAGAAAAGCCTCTGCCAACTCTAGGAAGAGGAAACCTTC
  CCCTTCTCCTCCTCCTCCCACAGCCACAGAGTCCCGCAAGAAGAGCGGGAAGAAAGGACAAGCTAGCCTT
  TATGGGAAACGTAGAAGTCAGAAGGAAGAAGATGAGCAAGAAGATCTTACCAAGGACATGGAAGACCCCA
  CACCTGTACCTAACATAGAGGAAGTGGTTCTCCCTAAGAATGTAAACCCAAAGAAGGACAGTGAAAACAC
  ACCCGTTAAAGGAGGCACGGTGGCAGATCTAGATGAGCAGGATGAAGAAGCAGTTACAACAGGAGGAAAG
  GAAGATGAAGATCCCAGCAAAGGTGATCCAAGTCGCTCAGTTGACCCAGGTGAAGACAACGTGACAGAAC
  AGACCAATCACATCATTATTCCCAGCTACGCATCCTGGTTTGATTATAATTGTATTCATGTCATTGAACG
  GCGTGCGCTTCCTGAGTTCTTTAATGGAAAAAACAAATCCAAGACCCCTGAAATATACTTGGCATATCGA
  AATTTTATGATTGACACATACCGTCTAAACCCTCAAGAATATTTAACCAGCACTGCTTGCCGGCGAAACC
  TGACTGGAGATGTGTGTGCTGTGATGAGGGTTCATGCCTTCTTAGAGCAGTGGGGTCTTGTTAACTACCA
  AGTTGACCCAGAGAGTCGACCCATGGCAATGGGACCTCCTCCCACTCCTCACTTCAATGTGTTAGCTGAC
  ACACCCTCTGGGCTTGTGCCCCTGCATCTTCGATCACCTCAGGTCCCTGCCGCTCAACAGATGTTAAATT
  TTCCTGAGAAGAACAAGGAAAAACCAATTGATTTGCAGAACTTTGGTCTTCGAACTGACATTTACTCCAA
  GAAAACACTGGCAAAGAGTAAAGGTGCTAGTGCTGGAAGGGAGTGGACAGAACAGGAGACCCTTCTTCTC
  CTAGAGGCTCTGGAGATGTACAAGGACGATTGGAATAAAGTGTCAGAACATGTTGGAAGCCGTACTCAGG
  ACGAATGCATCCTCCACTTTCTGAGGCTTCCCATTGAGGACCCTTACCTTGAAAATTCAGATGCTTCTCT
  TGGGCCACTGGCTTACCAGCCTGTCCCTTTCAGCCAGTCGGGAAACCCGGTGATGAGCACTGTTGCCTTT
  TTAGCATCTGTCGTTGACCCCCGTGTAGCATCTGCTGCAGCAAAAGCAGCGTTGGAGGAGTTTTCTCGTG
  TCCGAGAAGAAGTACCCCTGGAATTGGTTGAAGCACATGTCAAGAAAGTACAGGAAGCTGCAAGAGCCTC
  TGGGAAGGTGGACCCCACCTATGGCTTGGAGAGCAGCTGTATTGCTGGCACAGGGCCTGACGAGCCAGAG
  AAGCTTGAAGGATCTGAAGAAGAGAAGATGGAAACAGATCCTGATGGTCAGCAGCCTGAAAAGGCAGAAA
  ACAAAGTGGAAAATGAATCGGATGAAGGTGATAAAATACAAGATCGAGAGAATGAAAAAAACACTGAGAA
  GGAACAAGATAGTGACGTCAGTGAGGATGTCAAGCCAGAAGAAAAGGAGAATGAAGAGAACAAAGAGCTC
  ACTGATACATGTAAAGAAAGAGAAAGCGATGCCGGGAAGAAGAAAGTGGAACACGAGATTTCGGAAGGAA
  ACGTTGCCACAGCCGCAGCAGCTGCTCTGGCCTCAGCTGCTACTAAAGCCAAGCACCTGGCGGCTGTTGA
  AGAAAGAAAAATCAAGTCCTTGGTAGCTCTCTTGGTTGAAACACAAATGAAGAAACTAGAGATCAAACTT
  CGACATTTTGAAGAGCTGGAGACTATAATGGACAGAGAGAAAGAGGCTCTAGAACAACAGAGACAGCAGT
  TGCTTACTGAGCGTCAGAACTTCCACATGGAACAGTTGAAATATGCTGAACTACGTGCCCGGCAGCAAAT
  GGAGCAGCAGCAGCAGCATGGCCAGACACCTCAGCAGGCGCACCAGCACACGGGAGGGCCGGGGATGGCC
  CCACTTGGAGCCACAGGCCACCCTGGCATGATGCCGCATCAGCAGCCCCCTCCCTACCCACTGATGCACC
  ATCAGATGCCGCCACCCCATCCTCCCCAACCAGGTCAAATACCAGGCCCTGGCTCCATGATGCCTGGCCA
  GCCCATGCCAGGTCGCATGATCCCCGCTGTGGCAGCCAACATTCACCCTACTGGGAGTGGCCCTACCCCT
  CCTGGTATGCCTCCAATGCCCGGAAACATCTTAGGACCCCGGGTACCCCTCACAGCACCAAACGGCATGT
  ATCCTCCTCCACCACAGCAGCAGCAGCCGCCTCCTCCTGCAGATGGGGTCCCTCCACCTCCTGCTCCAGG
  CCCACCCGCCTCGGCCACTCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009211
• Insert Size: 3315 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009211.2, NP_033237.2
• RefSeq Size: 5684 bp
• RefSeq ORF: 3315 bp
• Locus ID: 20588
• UniProt ID: P97496
• Cytogenetics: 9 F2
• Gene Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function]