Pold1 (NM_011131) Mouse Untagged Clone

CAT#: MC223588

Pold1 (untagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pold1
• Synonyms: 125kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223588 representing NM_011131
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGTAAGCGGCGACAAGGACCAGGCCCTGGGGTGCCCCCAAAGCGGGCTCGAGGGCACCTCTGGG
  ATGAGGACGAGCCTTCGCCGTCGCAGTTTGAGGCGAACCTGGCACTGCTGGAGGAAATAGAGGCTGAGAA
  CCGGCTGCAGGAGGCAGAGGAGGAGCTGCAGCTGCCCCCAGAGGGCACCGTGGGTGGGCAGTTTTCCACT
  GCAGACATTGACCCTCGGTGGCGGCGGCCCACCCTACGTGCCCTGGACCCCAGCACGGAGCCCCTCATCT
  TCCAGCAGCTGGAGATTGACCACTATGTGGGCTCAGCACCACCCCTGCCAGAAGGGCCCCTGCCATCCCG
  GAACTCAGTGCCCATACTGAGGGCCTTTGGGGTCACCGATGAAGGCTTCTCCGTCTGCTGCCACATACAG
  GGCTTTGCCCCCTACTTCTACACCCCCGCGCCTCCTGGTTTTGGGGCCGAGCACCTGAGTGAGCTGCAGC
  AGGAGCTGAACGCAGCCATCAGCCGGGACCAGCGCGGTGGGAAGGAGCTCTCAGGGCCGGCAGTGCTGGC
  AATAGAGCTATGCTCCCGTGAGAGCATGTTTGGGTACCACGGTCATGGCCCTTCTCCATTTCTCCGCATC
  ACCCTGGCACTACCCCGCCTTATGGCACCAGCCCGCCGCCTTCTGGAACAGGGTGTCCGAGTGCCAGGCC
  TGGGCACCCCGAGCTTCGCACCCTACGAAGCCAACGTGGACTTTGAGATCCGGTTCATGGTGGATGCTGA
  CATTGTGGGATGCAACTGGTTGGAGCTGCCAGCTGGAAAGTACGTTCGGAGGGCGGAGAAGAAGGCCACC
  CTGTGTCAGCTGGAGGTGGACGTGCTGTGGTCAGATGTGATCAGTCACCCACCGGAGGGGCAGTGGCAGC
  GCATTGCACCCCTGCGTGTGCTTAGCTTCGACATCGAGTGTGCTGGCCGAAAAGGCATCTTCCCTGAGCC
  TGAGCGTGACCCCGTGATCCAGATCTGTTCTCTGGGGCTGCGCTGGGGGGAGCCGGAGCCATTCTTGCGT
  CTGGCACTCACGCTGCGGCCCTGTGCCCCCATCCTGGGTGCCAAAGTGCAGAGCTATGAGCGGGAAGAAG
  ACCTGCTCCAGGCCTGGGCCGACTTCATCCTTGCCATGGACCCTGACGTGATCACCGGCTACAACATTCA
  GAACTTTGACCTCCCATACCTCATCTCTCGGGCACAGGCCCTAAAGGTGGACCGCTTCCCTTTCCTGGGC
  CGCGTGACTGGTCTCCGCTCCAACATCCGTGACTCCTCCTTCCAATCAAGGCAGGTCGGCCGGCGGGACA
  GTAAGGTGATCAGCATGGTGGGTCGCGTTCAGATGGATATGCTGCAGGTGCTGCTTCGGGAACACAAGCT
  CCGCTCCTACACGCTCAACGCTGTGAGTTTCCACTTCCTGGGCGAGCAGAAGGAGGACGTTCAGCACAGC
  ATCATCACCGACCTGCAGAATGGGAACGAACAGACGCGCCGCCGCCTGGCCGTGTACTGCCTGAAGGACG
  CCTTTCTGCCACTCCGACTACTAGAGCGCCTTATGGTGCTGGTGAATAATGTGGAGATGGCGCGTGTCAC
  GGGTGTACCCCTTGGGTACCTGCTCACCCGGGGCCAGCAGGTCAAGGTCGTGTCTCAGCTGCTGCGCCAG
  GCCATGCGCCAGGGGCTGCTGATGCCTGTGGTGAAGACCGAGGGCAGTGAGGACTACACGGGAGCCACAG
  TCATTGAGCCCCTCAAAGGGTACTATGACGTCCCCATTGCCACCCTGGACTTCTCCTCCTTGTACCCATC
  CATCATGATGGCCCATAATCTGTGCTACACCACGCTGCTCCGACCTGGGGCTGCCCAGAAGCTGGGCCTT
  AAACCAGATGAGTTCATCAAGACACCCACTGGGGATGAGTTTGTGAAGTCATCTGTACGGAAGGGCCTCC
  TGCCCCAGATCCTGGAGAATCTGCTGAGTGCCCGCAAGAGGGCCAAGGCTGAGCTGGCTCAGGAGACGGA
  CCCCCTGCGGCGACAGGTCTTGGACGGCCGGCAACTGGCACTAAAAGTGAGTGCCAACTCCGTATATGGC
  TTCACTGGTGCCCAGGTGGGCAAGCTGCCATGTTTGGAGATCTCCCAGAGTGTCACTGGGTTCGGGCGGC
  AGATGATTGAGAAAACCAAGCAGCTTGTGGAGTCCAAGTACACCGTGGAAAATGGCTACGATGCCAACGC
  CAAGGTAGTCTACGGTGACACGGACTCTGTGATGTGCCGGTTTGGCGTCTCCTCTGTGGCTGAAGCAATG
  TCTCTGGGGCGGGAGGCTGCAAACTGGGTATCCAGTCACTTCCCATCACCCATCCGGCTGGAGTTCGAGA
  AGGTTTACTTCCCATACCTGCTCATCAGCAAGAAGCGCTATGCTGGCCTGCTCTTCTCCTCCCGCTCTGA
  TGCCCATGACAAAATGGACTGCAAGGGCCTGGAGGCTGTGCGCAGGGACAACTGTCCCCTGGTGGCCAAC
  CTCGTTACATCCTCTCTGCGCCGGATCCTCGTGGACCGGGACCCTGATGGGGCAGTAGCCCATGCCAAGG
  ACGTCATCTCGGACCTGCTGTGCAACCGCATAGACATCTCCCAGCTGGTCATCACCAAAGAGTTGACCCG
  CGCAGCAGCAGACTATGCTGGCAAGCAGGCTCACGTGGAGCTGGCTGAGAGGATGAGGAAGCGCGACCCC
  GGCAGTGCGCCCAGCCTGGGTGACCGAGTCCCCTATGTGATCATTGGTGCTGCTAAGGGTGTGGCCGCCT
  ACATGAAGTCGGAGGACCCCCTGTTTGTGCTGGAGCACAGCCTGCCCATCGACACTCAGTACTACCTGGA
  GCAGCAGCTGGCCAAGCCGCTCTTGCGCATCTTTGAGCCCATCCTGGGTGAGGGCCGTGCAGAGTCTGTG
  CTGCTGCGCGGTGACCACACACGATGCAAGACTGTGCTCACCAGCAAGGTGGGCGGCCTCTTGGCCTTCA
  CCAAGCGCCGCAACTGTTGCATTGGCTGCCGCTCCGTAATCGACCATCAAGGAGCCGTGTGTAAGTTCTG
  TCAGCCACGGGAGTCGGAGCTCTATCAGAAGGAGGTGTCACACCTGAATGCCTTGGAAGAACGGTTCTCT
  CGCCTCTGGACACAGTGTCAACGCTGCCAGGGCAGCTTGCATGAGGACGTCATCTGTACCAGCCGTGACT
  GTCCCATCTTCTACATGCGCAAGAAGGTGCGCAAGGACCTGGAAGACCAGGAACGGCTGCTGCAGCGCTT
  TGGACCGCCCGGCCCTGAGGCCTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011131
• Insert Size: 3318 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011131.3, NP_035261.3
• RefSeq Size: 3428 bp
• RefSeq ORF: 3318 bp
• Locus ID: 18971
• UniProt ID: P52431
• Cytogenetics: 7 28.83 cM
• Gene Summary: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites.[UniProtKB/Swiss-Prot Function]