Iqsec1 (NM_001134384) Mouse Untagged Clone

CAT#: MC223570

Iqsec1 (untagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Iqsec1
• Synonyms: AW561907; BC026481; BRAG2; cI-43; D6Ertd349e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223570 representing NM_001134384
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGCCGAAGACGCTATTTTGTTGAGGGCGAGGCCCCCAGCAGCGAGACTGGCACATCGCTGGACA
  GCCCCTCAGCCTACCATCAGGGTCCCCTGGTACCTGGTTCCAGCCTGAGTCCAGACCACTACGAGCACAC
  ATCAGTGGGCGCCTATGGGCTGTACGCAGGGCCAGGACCACAACAGCGCACACGGAGGCCCCGGCTGCAG
  CACTCGACCTCAGTCCTGCGCAAGCAGGCTGAGGAGGAGGCCATCAAGCGCTCTCGGTCACTCTCTGAGA
  GCTATGAGCTCTCTTCAGATCTGCAAGACAAGCAGGTGGAAATGCTAGAACGCAAGTATGGGGGACGCCT
  CGTGACCCGCCATGCAGCCCGCACCATCCAGACGGCGTTCCGCCAATACCAGATGAACAAGAACTTTGAG
  CGCCTGCGCAGCTCCATGTCGGAGAACCGCATGTCACGTCGCATTGTGCTGTCCAACATGAGGATGCAGT
  TCTCCTTTGAGGGCCCCGAGAAGGTGCACAGCTCCTACTTTGAGGGAAAGCAGGTCTCTGTGACTAATGA
  TGGCTCCCAGCTGGGGGCCCTGGTGCCATCAGAATGTGGAGACCTTAGTGACCCAGCCCTCAAGTCTCCA
  GCCCCCTCCAGTGACTTTGCAGATGCCATCACGGAACTGGAGGATGCCTTCTCCAGGCAGGTGAAATCCC
  TGGCTGAATCCATCGATGATGCCCTCAATTGCCGCAGCCTACACAGTGAGGAGGTACCAGCCTCAGACAC
  GGCCCGGGCTCGGGACACTGAGCCCAAACCAGGTCTACATGGCATGGACCACCGCAAACTGGATGAGATG
  ACAGCCTCGTATAGTGATGTGACCCTCTACATTGATGAGGAAGAGCTGTCACCACCGCTTCCACTCTCAC
  AGGCTGGCGATCGGCCCTCCAGCACAGAGTCGGACCTGCGGCTGAGGTCTGGGGGTGCAGCCCAGGACTA
  CTGGGCCTTGGCCCACAAAGAGGACAAGGCTGACACGGACACAAGCTGCCGAAGCACACCATCACTGGAG
  CGGCCAGAGCCACGGCTGCGGGTGGAGCACCTGCCCCTGCTTACCATTGAGCCACCCAGTGATAGCTCCG
  TGGAACTCAGTGACCGCTCAGATCGCAGCTCACTCAAGAGGCAGAGTGCCTATGAGCGCAGCCTCGGTGG
  GCAGCAGGGCAGCCCCAAGCATGGCCCCCACGGTGGCCCCCCCAAAGGCCTCCCCAGGGAGGAACCTGAA
  TTGCGGCCTCGGCCCCCCAGACCTCTTGAGAGTCACCTAGCCATCAATGGTTCAGCCAACCGGCAGAGCA
  AATCTGAGTCTGACTACTCAGATGGGGACAATGACAGCATCAACAGCACCTCCAACTCCAATGACACCAT
  AAACTGCAGCTCTGAGTCCTCATCACGGGACAGCCTGCGGGAACAGACACTCAGCAAGCAGACATACCAC
  AAGGAGACCCGCAACAGCTGGGACTCTCCAGCTTTCAGCAACGATGTCATCCGTAAGAGGCATTACCGAA
  TTGGCCTAAATCTCTTCAACAAGAAGCCTGAGAAGGGCATCCAGTATCTCATTGAGCGCGGCTTCGTGCC
  CGACACGCCAGTGGGAGTGGCCCACTTCCTGCTGCAGCGCAAGGGCCTCAGCCGCCAGATGATCGGTGAG
  TTCCTGGGAAACCGGCAGAAGCAGTTCAACCGTGATGTGCTCGACTGTGTTGTGGACGAGATGGACTTCT
  CTGCCATGGAGCTAGATGAAGCCCTCCGGAAGTTCCAGGCACACATCCGAGTCCAAGGGGAGGCTCAGAA
  AGTGGAGCGGCTCATCGAGGCATTCAGCCAGCGGTACTGTGTCTGCAACCCAGGGGTGGTGCGACAGTTC
  CGGAACCCAGACACCATCTTCATCCTGGCCTTCGCCATCATCCTGCTCAACACAGACATGTACAGCCCCA
  ATGTCAAGCCAGAGCGCAAAATGAAGCTGGAGGACTTTGTCAAGAACCTCCGAGGTGTGGATGACGGTGA
  AGACATTCCCCGGGAGACGCTAATTGGGATCTATGAGCGAATCCGTAAGCGAGAACTGAAGACCAACGAG
  GACCACGTGTCCCAGGTGCAGAAGGTTGAGAAGCTCATCGTGGGCAAGAAGCCGATTGGATCCCTGCATC
  ATGGTCTTGGCTGTGTGCTGTCTCTTCCCCATCGACGACTAGTCTGCTACTGCCGGCTTTTTGAAGTTCC
  TGACCCCAACAAACCCCAGAAGCTGGGACTACACCAGCGAGAGATCTTCCTGTTTAATGACCTCCTGGTG
  GTCACCAAGATCTTCCAGAAGAAGAAGAACTCGGTGACATACAGCTTCCGGCAGTCCTTCTCCCTCTACG
  GCATGCAGGTCCTGCTCTTCGAGAACCAGTACTATCCCAATGGCATCCGGCTGACGTCTGCTGTCCCTGG
  AGCAGACATCAAAGTGCTAATAAACTTCAACGCTCCCAATCCTCAGGACCGGAAGAAGTTCACTGACGAC
  CTGCGGGAGTCTGTCGCTGAAGTGCAAGAGATGGAGAAACACCGAATAGAGTCGGAGCTCGAGAAGCAGA
  AAGGTGTCGTGCGGCCCAGCATGTCACAGTGCTCCAGCCTCAAAAAGGAGTCAGGCAACGGGACACTGAG
  CAGGGCTTGCCTGGATGACAGCTATGCCAGTGGCGAGGGCCTCAAGCGCAGCGCCCTCAGCAGCTCACTG
  AGAGACCTCTCAGAAGCGGGGAAGCGAGGGCGTCGCAGCAGTGCGGGATCGCTAGAGAGCAATGTGGAAG
  GCTCCATCATTAGCAGTCCTCACATGCGCCGGAGAGCGACCTCAACACGAGAGTGCCCATCTCGCCCCCC
  CCAGCCCGTGCCTAACTCCTCCTCTCTCCTGGGTTCTTTGTTTGGGAGCAGGAGAGGGAAGCCCCCTCCC
  CAGGCCCACCCACCCTCAGCCCCTCCCCCAGCACCCCCTCACGCCCATCACCCTGGGCCCTCAGAAGGCC
  CACTGCATGGACATCATGCACAGTACTGCCATGCACAGCAGAATCCTCCGCCCTATCACCACCACCACCA
  CTACCACCCACCCCCCCACCTGCAGCACACCCACCAGTACCACCATGGCCCACACGGCGGGCACCCCCCG
  TATGGGGCCCATGCACACAGCCACCCGCCTCTGCCCACAGCTCACCCTGGTCATGCAGGGCACTCAGCGC
  ATCATCATGGGCAGCCCCCTGCCCCACCGCCCCCCACCAGCAGCAAGGCCAAGCCCAGCGGCATCAGCAC
  AGTTGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001134384
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134384.1, NP_001127856.1
• RefSeq Size: 6490 bp
• RefSeq ORF: 3300 bp
• Locus ID: 232227
• Cytogenetics: 6 40.16 cM
• Gene Summary: Guanine nucleotide exchange factor for ARF1 and ARF6. Guanine nucleotide exchange factor activity is enhanced by lipid binding. Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.