Plcl1 (NM_001114663) Mouse Untagged Clone

CAT#: MC223568

Plcl1 (untagged) - Mouse phospholipase C-like 1 (Plcl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plcl1
• Synonyms: C230017K02Rik; PLC-L; PLC-L1; PLCE; PLCL; PLDL1; PRIP; PRIP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223568 representing NM_001114663
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGGCGCGGCTAGCAGGGAGGCTCCGGCGCCTCTCGACGTTGCCGGGGGCGAAGACGACCCCC
  GAGCCGGCGCCGACGCTGCCTCAGGGGACGCCCCACCCCCGGCCTTGGGGGGCCGGATGAGGGACCGTCG
  CAGTGGGGTCGCGCTGCCGGGCGCTGCGGGGGTCCCCGCGGATAGCGAGGCCGGCCTCCTGGAGGCTGCA
  CGAGCGACCCCCCGGCGTAGCAGCATCATCAAGGATCCTTCAAACCAAAAATGTGGTGGCAGAAAGAAAA
  CAGTGTCTTTCAGTAGCATGCCATCGGAGAAGAAGATTAGCAGTGCCCACGACTGCATTAGCTTCATGCA
  GGCTGGCTGCGAGCTGAAGAAAGTGCGCCCGAACTCTCGCATCTACAACCGATTTTTCACCCTAGATACC
  GACCTCCAGGCTCTACGCTGGGAACCTTCCAAGAAAGACCTTGAAAAAGCCAAGCTGGACATTTCTGCCA
  TCAAAGAGATCAGGCTGGGGAAGAACACAGAGACATTCAGAAACAATGGCCTTGCTGACCAGATCTGTGA
  AGACTGTGCCTTCTCCATACTCCATGGGGAAAATTACGAGTCTCTGGACCTAGTTGCCAATTCAGCAGAT
  GTGGCAAACATCTGGGTGTCGGGGTTACGGTACCTGGTCTCTCGTAGTAAGCAACCGCTTGATTTTATAG
  AGGGTAACCAGAACACACCACGGTTCATGTGGCTGAAAACGGTGTTTGAAGCAGCAGATGTTGATGGAAA
  TGGGATTATGTTAGAAGACACTTCAGTGGAGTTAATAAAACAACTCAACCCTACTCTGAAGGAATCTAAG
  ATCAGGTTAAAATTTAAAGAAATCCAGAAGAGCAAAGAAAAACTAACTACCCGGGTGACAGAAGAGGAAT
  TTTGTGAAGCATTTTGCGAACTGTGCACCAGGCCAGAAGTGTACTTCTTACTTGTCCAGATATCTAAGAA
  CAAAGAGTACCTGGATGCCAATGACCTCATGCTCTTCTTAGAAGCTGAGCAAGGTGTCACCCACATCACT
  GAGGATATGTGTTTAGACATCATTAGGAGATATGAGCTCTCTGAAGATGGCCGCCAGAAAGGCTTCCTTG
  CAATTGATGGGTTTACCCAGTATTTATTGTCTCCAGAATGTGACATTTTTGATCCAGAGCAAAAAAAAGT
  TGCCCAAGATATGACCCAACCCTTATCTCACTACTATATCAATGCGTCTCATAATACCTACCTGATAGAA
  GATCAGTTCAGGGGACCAGCAGATATCAATGGGTATGTGAGAGCATTGAAGATGGGCTGTAGAAGCATTG
  AGCTCGATGTGAGTGATGGTCCGGATAATGAGCCCATCCTTTGTAATAGGAACAACATGGCCATGCACCT
  TTCCTTTCGAAGTGTTCTGGAGGTGATAAATAAGTTTGCCTTTGTTGCTTCAGAGTACCCACTCATTCTC
  TGCTTGGGGAATCACTGCTCTCTGCCTCAGCAGAAAGTCATGGCACAGCAGATGAAAAAGGTGTTTGGCG
  AGAAACTTTATACAGAAGCACCTTTGTCTTCAGAATCCTACCTCCCATCACCTGAAAAACTAAAAAATAT
  GATTATTGTGAAAGGAAAGAAATTGCCTTCGGAATCAGATCTGTTAGAAGGAGAAGTGACCGACGAGGAC
  GAAGAAGCCGAGATGTCGCGGAGGATGTCAGGGGATTACAACGGTGAGCAGAAACATATCTGGCTCTGCC
  GAGAGCTCTCTGACTTGGTATCCATCTGCAAATCTGTTCAGCACAGGGATTTTGAACTGTCTATGAAAAC
  CCAAAACTACTGGGAAATGTGTTCATTTAGTGAAACAGAGGCCAGCCGGATTGCCAATGAATACCCAGAG
  GACTTCGTGAATTATAACAAGAAGTTCTTATCAAGGGTCTACCCTAGTGCCATGCGGATAGATTCCAGTA
  ATTTGAACCCACAGGACTTCTGGAACTGTGGCTGTCAGATTGTGGCGATGAATTTTCAAACTCCCGGTCC
  AATGATGGACCTCCACACTGGCTGGTTTCTTCAAAATGGAGGATGTGGCTACGTCCTCAGGCCATCTATC
  ATGCGAGATGAAGTTTCGTACTTCAGCGCAAATACAAAGGGCATTGTCCCTGGCGTGTCTCCCCTGGTTC
  TTCACATCAAGATCATCAGTGGTCAGAACTTCCCAAAGCCCAAAGGAGCCTGTGCCAAAGGGGATGTCAT
  AGACCCCTATGTTTGTGTGGAGATTCACGGAATTCCAGCTGATTGCTGCGAGCAAAGAACTAAAACTGTA
  CAACAGAACAGCGATAATCCAATTTTTGATGAAACTTTCGAGTTTCAAGTCAACCTTCCCGAGCTGACCA
  TGGTCCGTTTTGTCATTTTGGATGATGATTATATTGGGGATGAGTTCATTGGGCAGTATACGATCCCATT
  TGAATGTCTGCAGCCCGGGTACCGGCACGTTCCCTTGCGCTCCTTTGTTGGGGATATCATGGAGCACGTG
  ACCCTTTTCGTTCACATAGCAATAACCAATCGCAGTGGAGGAGGAAAACCACAGAAGCGAAGCCTTTCTG
  TGAGAATGGGGAAGAAAGTTCGAGAATACACCATGCTCAGGAATATTGGTCTTAAAACCATAGATGATAT
  CTTCAAAATAGCAGTTCATCCCTTACGAGAAGCCATAGATATGAGAGAAAACATGCAGAACGCCATAGTG
  TCTGTTAAGGAGCTGTGTGGACTCCCGCCGATTGCCAGTCTGAAACAGTGCCTGTTAACCCTGTCCTCTC
  GTCTCATCACCAGTGACAGTACTCCCTCTGTGTCTCTGGTGATGAAGGACTGCTTTCCTTACCTGGAGCC
  TCTGGGTGCCATTCCAGATGTGCAGAAGAGGATGCTGGCTGCATATGATCTGATGATTCAAGAGAGCCGG
  GTCCTCATAGAAATGGCTGACACAGTTCAGGAAAAGATTGTACAGTGTCAGAAAGCAGGGATGGAATTCC
  ATGAGGAACTTCATAATCTGGGGGCAAAGGAAGGCTTGAAGGGAAGAAAACTCAACAAGGCCATTGAGAG
  CTTCGCTTGGAATATTACGGTATTGAAGGGACAAGGAGACCTGCTGAAGAATGCCAAGAATGAAGCCGTG
  GAGAACATAAAGCAAATCCAGCTGGCCTGCTTGTCTTGTGGACTGAGTAAAGGCCCTGGCGGTGGCTCAG
  AGGCCAAGGGCAAGCGCAGCTTGGAAGCGATAGAGGAGAAAGAAAGTAGTGAGGAGAACGGGAAGCTGTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001114663
• Insert Size: 3291 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114663.1, NP_001108135.1
• RefSeq Size: 6555 bp
• RefSeq ORF: 3291 bp
• Locus ID: 227120
• UniProt ID: Q3USB7
• Cytogenetics: 1 C1.2
• Gene Summary: Involved in an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor. Acts as an inhibitor of PPP1C (By similarity). Involved in the assembly and/or the trafficking of gamma-2 subunit-containing GABA A receptors.[UniProtKB/Swiss-Prot Function]