Sin3b (NM_009188) Mouse Untagged Clone

CAT#: MC223551

Sin3b (untagged) - Mouse transcriptional regulator, SIN3B (yeast) (Sin3b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sin3b
• Synonyms: 2810430C10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223551 representing NM_009188
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCATGCAGGCAGCGGAGGCTCCGCGGGCCGGGGATTCGGTGGCTCCCGCTGGGGTCGTTCGGGCT
  CCGGAGGGCACGAGAAGCTGCCGGTGCACGTGGAGGACGCTCTCACCTACCTCGACCAGGTGAAGATCCG
  CTTTGGCAGTGACCCTGCCACCTACAACGGCTTCCTGGAGATCATGAAGGAGTTCAAAAGCCAGAGCATC
  GACACTCCTGGTGTGATCAGGCGTGTGTCCCAGCTCTTCCATGAGCACCCTGACCTCATCGTGGGGTTTA
  ATGCTTTCCTTCCGCTTGGATACCGTATAGACATTCCCAAGAACGGCAAGCTGAACATCCAGTCGCCGCT
  GAGCAGCCAGGACAACTCCCATAGCCATGGTGACTGTGGGGAGGACTTCAAGCAGATGTCCTACAAGGAG
  GACAGAGGCCAGGTGCCCCTGGAGTCTGACTCTGTGGAGTTCAACAATGCTATCAGCTATGTGAACAAGA
  TCAAGACCCGCTTTCTGGACCACCCTGAGATCTACAGGTCCTTCCTGGAGATCCTACACACCTACCAGAA
  GGAACAGCTGCACACTAAGGGCCGTCCATTCCGTGGCATGTCTGAGGAGGAGGTTTTCACAGAGGTGGCC
  AATCTTTTCCGTGGTCAGGAGGACCTGCTGTCAGAGTTCGGACAGTTCCTGCCTGAGGCAAAGCGGTCCC
  TGTTTACAGGCAATGGGTCGTGCGAGATGAACAGTGGGCAGAAGAATGAGGAGAAGAGTCTGGAACACAA
  CAAGAAACGCTCTCGGCCTTCACTCCTGCGCCCTGTGTCTGCGCCAGCCAAGAAGAAGATGAAGCTCCGA
  GGCACCAAAGACCTGTCCATTGCTGCCGTGGGGAAATACGGCACACTGCAGGAATTCTCCTTCTTTGACA
  AGGTGCGCAGGGTGCTGAAGAGCCAGGAGGTATATGAGAACTTTCTGCGCTGCATTGCGCTCTTCAATCA
  GGAGCTGGTGTCCGGGTCTGAGTTGCTGCAGCTTGTCAGTCCATTCCTGGGGAAATTTCCAGAACTCTTT
  GCACAGTTCAAGTCCTTCCTAGGAGTGAAAGAGCTGTCCTTTGCACCACCTATGAGTGACCGGTCTGGAG
  ATGGGATAAGCAGAGAAATTGACTATGCGTCTTGTAAGCGAATAGGATCCAGCTATCGGGCACTTCCCAA
  GACCTACCAGCAGCCCAAGTGCAGCGGAAGGACTGCCATTTGCAAGGAGGTCCTGAATGATACATGGGTC
  TCCTTTCCATCCTGGTCTGAAGACTCCACCTTTGTTAGCTCCAAGAAGACGCCCTACGAGGAGCAACTGC
  ATCGCTGTGAGGATGAGAGATTTGAATTGGATGTTGTCCTGGAGACCAACCTGGCCACCATCCGAGTGCT
  GGAGAGTGTGCAGAAGAAGTTGTCACGGATGGCTCCTGAGGACCAGGAGAAGCTCCGCCTGGATGACTGC
  CTGGGTGGCACATCAGAGGTGATTCAACGCCGTGCCATACACCGCATCTATGGAGACAAGGCCCCAGAGG
  TCATTGAGAGCCTGAAAAGGAACCCTGCCACCGCTGTGCCTGTCGTTCTGAAAAGGCTGAAGGCCAAGGA
  GGAGGAGTGGCGGGAGGCCCAGCAGGGCTTCAACAAGATCTGGCGTGAGCAGTACGAGAAAGCATACCTC
  AAGTCCCTGGACCACCAGGCTGTGAACTTCAAGCAGAATGACACCAAGGCACTGCGCTCTAAGAGCCTGC
  TCAATGAGATCGAAAGTGTCTATGATGAGCACCAGGAGCAGCACTCAGAGGGCCGCAGTGCGCCTTCCAG
  CGAGCCGCACCTTATCTTCGTGTATGAGGACCGACAGATCCTAGAGGACGCAGCAGCACTCATCAGCTAC
  TATGTTAAGCGGCAGCCAGCCATTCAGAAAGAGGACCAGGGCACTATCCGCCAGCTGCTTCACCGTTTCC
  TGCCCAGCCTGTTCTTCTCGCAGCAGTGCCCCGGCACCTCCGATGACTCTGCTGATGAGCGCGACCGGGA
  CCGGGACAGCGCTGAGCCTGAGCGCCGCCGGCCCACAGATGAGAAGCCCCCTGCAGATGCATCTCCAGAA
  CCGCCCAAGGTCCTGGACGATGTGTACAGTCTGTTTTTTGCCAACAACAACTGGTACTTCTTCCTGCGGC
  TGCACCAGACGCTGTGCGCCCGCCTGCTCAAGATCTACCGGCAGGCGCAGAAGCAGCTGCTGGAACATCG
  ACGAGAGCAGGAGAGGGAGCAGCTGCTGTGCGAGGGCCGCCGTGAGAAGGCTGCAGACCCTGCCATGGAG
  CTGCGACTCAAGCAGCCTAGTGAAGTGGAGCTGGAGGAGTACTACCCGGCCTTCCTGGACATGGTGCGGA
  GCCTGCTAGAGGGGAGCATCGACCCCACACAGTATGAGGACACTCTCCGTGAGATGTTCACCATCCATGC
  CTACATTGGCTTCACTATGGACAAGCTAGTGCAGAACATTGCCCGCCAGCTACACCATCTGGTGAGTGAC
  GACGTATGTCTGAAGGTGGTAGAGCTGTACCTGAATGAGCAGCAGCGAGGGGCAGCGGGGGGAAACCTGT
  CCTCCAGATGTGTTCGTGCAGCACGGGAGACTAGCTATCAGTGGAAGGCGGAGCGCTGCATGGCAGATGA
  GAACTGCTTCAAGGTAATGTTCCTGCAGCGCAGAGGTCAGGTGATCATGACCATAGAGCTCCTGGACACA
  GAAGAGGCACAGACTGAGGACCCTGTGGAGGTACAGCACCTGGCTCGCTATGTGGAGCAGTATGTGGGGT
  CTGAGGGTGCATCCAGCTCATCTACTGAAGGCTTCCTGCTGAAGCCGGTGTTCCTGCAGAGGAACCTGAA
  GAAGTTCCGGCGTTGGCAGTGTGAGCAGGTACGTGCCATGCGCGGTGAGGCTAAGAGCTCCTGGAAGCGG
  CTCATGGGCGTGGAGAGCGCTTGTGATGTGGACTGCCGGTTCCGCTTGGGCACACACAAGATGGTGTTCA
  TCGTCAATTCCGAGGACTACATGTACCGCCGTGGGACACTATGCCGTGCCAAGCAGGTGCAGCCCCTGGT
  GTTGTTGCGCCACCACCGGCACTTCGAGGAGTGGCATGGTCGCTGGCTGGAGGACAATGTAACTGTGGCG
  GCGGCAGGGCTGGTGCAGGACTGGCTGATGGGTGAGGAAGAGGAGGACATGGTACCCTGCAAGACGCTTT
  GCGAGACTGCGCATGTTCACGGGCTGCCGGTGACTCGCTACCGAGTGCAGTACAGCCGCCGCCCTGCTTC
  ACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009188
• Insert Size: 3297 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009188.4, NP_033214.2
• RefSeq Size: 4135 bp
• RefSeq ORF: 3297 bp
• Locus ID: 20467
• UniProt ID: Q62141
• Cytogenetics: 8 35.08 cM
• Gene Summary: Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression (PubMed:22476904).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer variant and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.