Ank3 (NM_170687) Mouse Untagged Clone

CAT#: MC223502

Ank3 (untagged) - Mouse ankyrin 3, epithelial (Ank3), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ank3
• Synonyms: 2900054D09Rik; AI314020; An; Ank; Ank-3; AnkG; Anky; Ankyrin-3; Ankyrin-G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223502 representing NM_170687
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGCCACACAGTGAAGATGCCATCACAGGGGACACTGACAAGTATCTCGGGCCACAGGACCTTA
  AGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTTACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAG
  CCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGAAGCTCCTACGCAAGGGACAGCATGATG
  ATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCACCTGACGTTCACGAGGGAGTTTGATTCTGACTCCC
  TCAGACACTACAGTTGGGCAGCGGACACGTTAGATAATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGG
  GTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATGCGAGGAAGCCGCCACCACGGGATGCGG
  ATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACCCGCATCACGTGCCGCCTGGTAAAGAGACATAAAC
  TGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGG
  GGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCCTCATTTTGGGTCCATGAGGGGGAAGGAGAGAGAA
  CTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGGAAGGAACATCAGTTTGACAGTAAAAACGAAGACC
  TCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCGACAGCCCGGAAGAGTTGGGTACAAAGCGCATCTG
  CAGAATTATCACAAAGGATTTCCCCCAGTATTTTGCCGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAG
  ATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACCGTGCCCCTCGTCCAGGCCTCCTTCCCAGAGGGCG
  CCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTCAGCCCGTGCCAGAGGAAACGGTAAAAAAAATCCT
  TGGGAACAAAGCAACATTTAGCCCAATTGTCACGGTAGAGCCGAGGAGAAGGAAGTTCCATAAGCCGATC
  ACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAAGGCGTGTCCAATGGGTACAAGGGGGATGCCACGC
  CCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCACCTCACCAGCTCAATGGGAAGACATCACAGGAAC
  AACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTTCACAACCAACGTTTCAGCCAGATTCTGGCTGGCG
  GACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCCTCCCAGCTGTACAGAGAGCTGATATGCGTTCCCT
  ACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACGACCCGGTGGAGTCCTCGCTGAGGTGCTTCTGTAT
  GACAGACGACAGGGTGGACAAAACCCTGGAGCAGCAGGAGAACTTCGAGGAGGTTGCCAGAAGCAAAGAC
  ATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGATTGCTATGGAAACCTGGCCCCTCTGACCAAAGGAG
  GACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAGAAAACAGACTGCCATTTTCCATCAAGATCAGAGA
  CACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCTGAAGGAGCCAAAGACAACAAAGGGATTACCCCAA
  ACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCACATAAAAAGGCTGAGAAGGCAGACAGACGCCAGA
  GCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAACCCAGCATGAGTCCGCAGAGTCC
  TTGTGAGCGGACGGATATCAGGATGGCGATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAGCTGGCA
  AGGGAACTGAATTTTTCAGTGGATGAAATCAACCAAATACGTGTGGAAAATCCCAATTCTTTAATTTCTC
  AGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAGACGGAAAGAATGCCACAACTGATGCCTTAACTTC
  GGTCTTAACGAAGATTAACCGGATAGACATTGTAACTCTGCTGGAAGGACCAATATTTGATTATGGGAAT
  ATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAATGTTTTCCATGACCCAGTTGATGGTCACCCTTCCT
  TTCAAGTGGAGCTGGAGACCCCCATGGGGTTGTACTGCACACCACCCAACCCTTTCCAGCAAGATGACCA
  TTTTAGTGATATCTCTAGCATAGAGTCTCCCTTTAGGACCCCCAGTAGACTGAGTGACGGGCTGGTGCCT
  TCCCAGGGAAACATAGAGCATCCAACAGGTGGACCTCCAGTGGTAACCGCAGAGGACACTTCTTTAGAAG
  ACAGCAAAATGGACGATTCTGTAACTGTAACAGACCCGGCCGACCCACTGGACGTAGATGAGAGCCAGTT
  GAAGGACCTGTGTCAGAGCGAGTGTGCTCAGTGCTGGGCGAGTGTGCCCGGGATCCCAAACGACGGTCGG
  CAGGCAGAGCCACTGAGACCGCAGACTAGAAAAGTAGGCATGAGCTCTGAACAGCAGGAAAAAGGAAAAT
  CTGGTCCTGATGAGGAAGTGACAGAAGACAAGGTCAAATCTCTGTTTGAGGACATTCAACTTGAAGAAGT
  AGAGGCTGAGGAGATGACAGAAGACCAGGGGCAGGCTATGCTTAACCGTGTTCAGCGAGCAGAACTGGCA
  ATGTCTTCACTTGCAGGTTGGCAGAACGAGACGCCAAGTGGAAGCCTAGAGTCCCCAGCGCAAGCTCGAA
  GACTAACTGGTGGGTTACTGGACCGTCTGGATGACAGCTCTGACCAGGCTCGGGATTCTATTACCTCATA
  CCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGGAAACCACACAGCGGAAGTCATTCCAGAAGCAAAG
  GCAAAACCCTACTTCCCGGAATCCCAAAACGATATAGGGAAACAGAGCATCAAGGAGAACCTGAAACCAA
  AAACACACGGATGTGGTCGCACTGAGGAACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCTGGAAGA
  AACCAGCAAGCTTGTCATAGAAGACGCACCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATGACCAGG
  ACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAAGAGGGGTCCACCAGGTCAGAGCCTAAGC
  AGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAATCCGGAACGTGGAGAAGAAAACCCACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_170687
• Insert Size: 3285 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170687.3, NP_733788.2
• RefSeq Size: 7554 bp
• RefSeq ORF: 3285 bp
• Locus ID: 11735
• UniProt ID: G5E8K5
• Cytogenetics: 10 36.1 cM
• Gene Summary: This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (6) utilizes an alternate exon in the 5' coding region and an alternate translation initiation site, compared to variant 2. This results in a shorter isoform (f) and distinct N-terminus, compared to isoform b.