Depdc5 (NM_177786) Mouse Untagged Clone

CAT#: MC223497

Depdc5 (untagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Depdc5
• Synonyms: AV016528
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223497 representing NM_177786
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACCACAAAGGTCTACAAACTTGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG
  TCGTGAACCCTAAAGTATTTCCTCACATCAAACTTGGAGACATTGTGGAGATTGCTCACCCCAATGATGA
  GTACAGTCCTTTGCTTTTGCAAGTCAAGTCGCTTAAGGAAGATTTACAGAAAGAAACTATCAGTGTGGAC
  CAGACTGTGACTCAAGTATTCCGGCTAAGACCTTATCAAGATGTCTATGTGAATGTTGTAGACCCCAAGG
  ATGTGACTCTTGACCTGGTGGAATTGACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT
  AAAGAAAAGTTTGGTGAGCACCTGTGCCTATATCACTCAAAAAGTGGAATTTGCTGGCATCAGGGCACAG
  GCTGGTGAGCTGTGGGTCAAGAATGAGAAGGTCATGTGTGGTTACATTAGTGAAGAGACCAGGGTGGTGT
  TCCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA
  TGGGGATCTGTACTTTGAGAAAGCTGTGAACGGTTTCCTCGCCGACCTGTTTACTAAATGGAAGGAGAAG
  AACTGTAGTCATGAAGTGACTGTGGTCCTGTTTTCCAGAACTTTCTATGATGCAAAATCTATTGATGAAT
  TTCCTGAAATAAACCGAGCTTCAATTCAAGAGGATCACAAAGGGAGATTCTATGAGGACTTTTACAAAGT
  GGTGGTGCAGAATGAGAGAAGGGAAGAGTGGACTTCACTCCTCGTGACCATTAAAAAACTCTTCATCCAG
  TATCCAGTGTTGGTGCGACTGGAACAGGCAGGGGGCTTTCCTCAAGGAGACAATTCTACCTCAGCACAAG
  GAAACTACCTAGAGGCCATCAACTTGTCATTCAATGTGTTTGACAAGCACTACATCAACCGAAACTTTGA
  CCGAACTGGGCAGATGTCTGTGGTGATCACGCCGGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG
  ATCTTGACCAAGCAGCGGATGATTGATAATGGAATTGGTGTGGACTTAGTGTGCATGGGAGAGCAGCCAC
  TACATGCTGTGCCATTATTCAAGCTACATAACCGGAGTGTTCCGAGGGATTCTCGACTGGGTGATGATTA
  TAATATTCCTCACTGGATAAACCATAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAACAGTTTCACT
  CCACGGATAAAACTAGCAGGAAAGAAGTCTGCCTCTGAGAAAACCAAAAATGGTCGTGATACATCTCTCG
  GGACTCCAAAGGAATCTGAAAACACCCTTCCCATCCAAGTAGATTATGATGCCTACGATGCTCAAGTGTT
  CAGGCTGCCTGGCCCATCCCGGGCTCAGCGCCTCGCCACCTGCAGGTCTGTGAGAGAACAGGAGAATCAC
  AGTCGCAAAAGTGCAAGCTCCTGTGATGTCTCATCCAGCCCTTCCCTGCCAAGCCGTGCACTGCCCACTG
  AGGAAGTGAGGAGCCAGGCTTCTGATGACAGCTCCCTGGGCAAGAGCACCAACATCCTGATGATCCCTAA
  CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCACTGGGCTACACCAGCACCCGAGATGTCCTGGAGAAC
  ATGATAGAACCCCCACAGAGGGACTCCAGTGCACCAGGAAGGTTCCATGTGGGTAGTGCAGAGTCCATGC
  TCCATGTCCGACCTGGAGGATACACGCCTCAGAGAGCGCTGATTAACCCCTTCGCCCCCTCGAGGATGCC
  CATGAAGCTCACCTCCAACAGAAGGCGCTGGATGCACACTTTTCCTGTAGGCCCATCTGGAGAGGCCATC
  CAGATCCATCATCAGACCCGGCAAAACATGGCAGAGCTGCAGGGCAGCAGGCAGAGGGACCCTACCCACT
  CCTCTGCAGAGCTGTTGGAGTTGGCATACCATGAAGCTGCCGGGAGGCACAGCACTTCCCGCCAGCCTGG
  TGACAGCATGTCCTTGAACTTCAGTGGAACGGAAGAGCTTTCTGTCAGCCTGCTTAGCAACAGCAGTACA
  GGTGTGAATCCTAGGACCCAGAACAAGGACTCCCTAGAGGACAGTGTTTCTACCTCTCCAGACCCAATGC
  CAGGCTTCTGTTGCACAGTTGGAGTGGATTGGAAGTCTCTTACTACTCCTGCATGCCTTCCCCTCACCAC
  TGACTACTTCCCGGACCGTCAGGGCCTGCAGAATGACTACACAGAGGGCTGCTATGATCTCCTCCCAGAA
  GCAGACATGGACAGGAGGGATGAGGAGGGTGTTCAAATGACAGCCCAGCAAGTGTTTGAAGAGTTCATCT
  GCCAGCGTCTCATGCAGGGTTACCAAATCATAGTACAGCCAAAGACCCAGAAACCCAACACCACAGTCCC
  ACCCCCATTGAGCAGTAGCCCACTCTATAGCAGAGGCCTTGTGTCCCGAAATCGCCCTGAGGAGGAAGGC
  CAGTATTGGTTAAGTATGGGCAGAACTTTCCATAAGGTGACACTCAAGGACAAGATGATCACAGTAACTC
  GTTACCTTCCCAAGTACCCTTATGAATCTGCCCAGATCCATTACACCTACAGCCTCTGCCCTTCCCACTC
  TGACTCGGAGTTTGTCTCCTGTTGGGTGGATTTCTGCCACGAACGGCTCGAGGAATACAAGTGGAATTAC
  TTAGATCAGTATATTTGTTCTGCTGGCTCTGAAGACTTCAGCTTAATCGAGTCTCTGAAGTTCTGGAGAA
  CCCGATTTTTACTACTGCCAGCCTGTGTTACTGCCACCAAGCGCATCACAGAAGGGGAGGTGCACTGTGA
  CATCTATGGGGACAAACCCCGTGCAGATGAAGATGAGTGGCAGCTTCTAGATGGCTTTATTCGCTTTGTA
  GAGGGCTTAAATCGCATCCGCAGGCGCCACCGCTCAGACCGCATGATTCGGAAGGGGACTGCCATGAAAG
  GCTTGCAGATGACCGGGCCCATCTCTGCACACTCTCTCGAGGCAGCAGGCCCTCCAGTCGGCAAGAAAGG
  AACCTCAGCTCTGTCTGCCCTGCTGGAGATGGAAGCTAGTCAGAAGAGCCTAGGAGAACAGCAGACGACT
  GTGCACGGAAAAAGCTCCACTCAGCCAGCTGAGAACAGCAGTGTTGCCATGACTCCCACCTATGTGGACA
  GCCCACGCAAGGTGCAGCATGTGACCCGCACAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177786
• Insert Size: 3258 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177786.4, NP_808454.2
• RefSeq Size: 3569 bp
• RefSeq ORF: 3258 bp
• Locus ID: 277854
• Cytogenetics: 5 17.35 cM
• Gene Summary: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.