Tshz3 (NM_172298) Mouse Untagged Clone

CAT#: MC223495

Tshz3 (untagged) - Mouse teashirt zinc finger family member 3 (Tshz3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tshz3
• Synonyms: A630038G13Rik; mKIAA1474; teashirt3; Tsh3; Zfp537
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223495 representing NM_172298
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGGAGGAAGCAGCAGGCGCCCCGGCGCGCAGCAGCCTATGTTTCCGATGAGTTAAAGGCAGCTG
  CCTTGGTAGAAGATGACGTAGAGCCGGAGGAACAAGCGGCAGATGGAGAGCCCTCAGCCAAGTACATGTG
  TCCAGAGAAGGAACTCAGCAAGGCCTGCCCTAGCTACCAGAACTCACCGGCCGCTGAGTTCTCCAGCCAT
  GAAATGGACAGCGAGTCTCACATCAGTGAGACGAGTGACCGGATGGCTGACTTTGAAAGCAGTTCCATCA
  AGAATGAGGAGGAGACCAAGGAGGTCCAGGTGCCCCTGGAGGACACCATCGTGTCTGATAGCCTGGAGCA
  GATGAAGGCCGTGTACAACAACTTCCTGTCCAATTCCTATTGGTCCAACCTCAACCTGAACCTGCACCAG
  CCCTCCTCCGAGAACAATGGTGGCAGCAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCTGTGGCAGCGGGA
  GCTTTGACTGGCACCAGAGTGCCATGGCGAAGACCCTGCAGCAGGTGTCCCAGAACCGAATGCTGCCGGA
  GCCCAGCCTGTTCAGCACCGTGCAGCTGTACCGCCAGAGCAGCAAGCTGTACGGCTCCATTTTCACCGGG
  GCCAGCAAGTTCCGTTGCAAAGACTGTAGCGCCGCCTACGACACCCTGGTAGAGCTGACCGTGCACATGA
  ATGAGACAGGTCACTATCGCGATGACAACCACGAGACCGACAACAACAACCCCAAGCGCTGGTCCAAGCC
  TCGCAAACGGTCTTTGTTGGAGATGGAGGGGAAGGAGGATGCTCAGAAGGTGCTCAAGTGTATGTACTGT
  GGCCACTCCTTTGAGTCCCTCCAAGACCTGAGCGTCCATATGATCAAAACTAAACACTACCAAAAAGTGC
  CTCTCAAGGAACCTGTCACACCCGTTGCAGCCAAAATCATCCCTGCTGCTCGGAAGAAACCTTCTCTGGA
  GCTGGAGCTGCCTAGCTCCCCTGATTCCACAGGTGGAACCCCCAAAGCCACCCTGTCTGATGCCAGCGAC
  GCCCTGCAGAAGAACTCCAACCCTTACATCACGCCAAATAATCGGTACGGCCACCAGAACGGGGCCAGCT
  ATGCATGGCACTTCGAGGCCCGAAAGTCTCAGATCCTCAAATGCATGGAGTGTGGAAGCTCCCATGACAC
  GCTCCAGGAGCTCACAGCTCACATGATGGTCACTGGCCACTTTATCAAGGTCACAAACTCGGCCATGAAG
  AAGGGGAAGCCCATCATGGAGACACCGGTCACGCCCACCATCACCACCTTGCTGGACGAGAAGGTGCAGT
  CTGTGCCACTGGCAGCCACCACATTCACGTCTCCCTCCAATACGCCCGCGAGTGTCTCCCCGAAGCTGGC
  GGTGGAGATCAAAAAGGAAGTGGACAAGGAGAAAGCAGTCCCGGATGAGAAACCCAAGGAGAGAGAGAAG
  CCCAGTGAAGAGGAGGAGAAGTATGATATTTCTTCCAAGTACCACTATTTGACTGAAAACGACCTAGAAG
  AGAGCCCTAAAGGTGGACTAGATATCCTCAAATCCCTTGAGAACACAGTAACGTCTGCCATCAACAAGGC
  TCAGAATGGCACTCCCAGCTGGGGCGGCTATCCCAGCATCCACGCTGCCTACCAGCTCCCCAACATGATG
  AAGTTGTCCCTGGGTTCCTCGGGGAAGAGCACGCCCCTGAAACCCATGTTTGGCAACAGCGAAATCGTGT
  CTCCCACAAAAACCCAGACCCTGGTCTCTCCACCCAGCAGCCAGACCTCTCCCATGCCCAAGACAAACTT
  TCACGCCATGGAGGAACTGGTGAAAAAAGTCACGGAGAAAGTTGCCAAAGTTGAGGAGAAAATGAAAGAG
  CCAGAAGGCAAGCTCTCGCCACCCAAGCGGGCCACGCCGTCCCCGTGCAGCAGCGAGCAGAGCGAACCCA
  TCAAGATGGAGGCCTCCAGCGACGGCAGCTTCAAAAGTCAGGAGAATAGCCCCAGCCCACCAAGAGATGC
  GTGCAAGGAGGCGAGCCCCTCCGCAGAGCCCGTAGAGAATGGCAAGGAGCTGGTGAAGCCCCTGAGCGGA
  GGTCTCAGCGGCAGCACAGCCATTATCACGGACCACCCACCAGAACAGCCTTTTGTGAACCCCCTGAGTG
  CCCTGCAGTCGGTCATGAACATCCACCTGGGCAAGGCCGCCAAGCCCTCGCTGCCTGCGCTGGACCCCAT
  GAGCATGCTTTTCAAGATGAGCAACAGTCTGGCCGAGAAGGCAGCTGTGGCCACCCCACCACCCCTTCAG
  GCCAAGAAGGCGGAGCATCTGGACCGCTATTTCTACCACGTCAACAATGACCAGCCCATAGACTTGACAA
  AAGGGAAGAGCGACAAAGGTTGCTCTTTAGGTTCAGGGCTTTTGTCCCCCACGTCCACATCCCCGGCAAC
  TTCCTCATCCACGGTGACAACGGCAAAGACATCTGCCGTCGTATCATTCATGTCAAACTCGCCGCTGCGT
  GAGAATGCCTTGTCAGATATATCCGATATGTTGAAGAACTTGACAGAGAGCCACACGTCAAAATCCTCCA
  CTCCTTCCAGCATCTCTGAGAAGTCTGACATTGACGGGGCCACCCTGGAAGAGGCCGAGGAGTCGACTCC
  GGCGCAGAAGAGGAAAGGCCGCCAGTCAAACTGGAATCCTCAGCACCTGCTAATCCTGCAGGCGCAGTTT
  GCAGCTAGCCTGCGGCAGACTTCAGAAGGGAAGTACATCATGTCCGATCTGAGCCCCCAGGAGCGCATGC
  ACATCTCCAGGTTCACCGGTCTCTCCATGACCACCATCAGCCATTGGCTGGCCAATGTGAAGTACCAACT
  TCGAAGGACAGGTGGAACAAAGTTCCTCAAAAACTTGGACACTGGACACCCAGTGTTCTTTTGTAACGAC
  TGTGCGTCACAAATCAGGACTCCTTCCACGTACATCAGCCACCTAGAGTCACATCTGGGCTTCCGGCTAC
  GGGACTTATCAAAATTGTCCACCGAACAGATTAACAATCAAATAGCACAAACCAAGTCGCCGTCTGAAAA
  ACTGGTGACATCCTCCCCGGAGGAGGATCTGGGGACCACCTACCAGTGCAAACTTTGTAATCGGACCTTT
  GCGAGCAAGCATGCTGTTAAACTTCACCTTAGCAAAACCCACGGGAAATCACCGGAAGACCACCTCCTGT
  TCGTTTCTGAATTGGAGAAGCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172298
• Insert Size: 3246 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172298.2, NP_758502.1
• RefSeq Size: 5002 bp
• RefSeq ORF: 3246 bp
• Locus ID: 243931
• UniProt ID: Q8CGV9
• Cytogenetics: 7 B2
• Gene Summary: Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter. Involved in the modulation of glutamatergic synaptic transmission and long-term synaptic potentiation (PubMed:27668656).[UniProtKB/Swiss-Prot Function]