Rest (NM_011263) Mouse Untagged Clone

CAT#: MC223476

Rest (untagged) - Mouse RE1-silencing transcription factor (Rest), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rest
• Synonyms: 2610008J04Rik; AA407358; NRSF; REST4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223476 representing NM_011263
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCCAGGTGATGGGGCAGTCTTCTGGAGGAGGCAGTCTCTTCAACAACAGTGCCAACATGGGCA
  TGGCCTTAACCAACGACATGTACGACCTGCACGAGCTCTCGAAAGCTGAACTGGCAGCCCCTCAGCTCAT
  CATGTTAGCCAACGTGGCCCTGACGGGGGAGGCAAGCGGCAGCTGCTGCGATTACCTGGTCGGTGAAGAG
  AGGCAGATGGCCGAATTGATGCCCGTGGGAGACAACCACTTCTCAGAAAGTGAAGGAGAAGGCCTGGAAG
  AGTCGGCTGACCTCAAAGGGCTGGAAAACATGGAACTGGGAAGTTTGGAGCTAAGTGCTGTAGAACCCCA
  GCCCGTATTTGAAGCCTCAGCTGCCCCAGAAATATACAGCGCCAATAAAGATCCCGCTCCAGAAACACCC
  GTGGCGGAAGACAAATGCAGGAGTTCTAAGGCCAAGCCCTTCCGGTGTAAGCCTTGCCAGTACGAAGCCG
  AATCTGAAGAGCAGTTTGTGCATCACATCCGGATTCACAGCGCTAAGAAGTTCTTTGTGGAGGAAAGTGC
  AGAGAAACAGGCCAAAGCCTGGGAGTCGGGGTCGTCTCCGGCCGAAGAGGGCGAGTTCTCCAAAGGCCCC
  ATCCGCTGTGACCGCTGTGGCTACAATACCAACCGGTATGACCACTACATGGCACACCTGAAGCACCACC
  TGCGAGCTGGCGAGAACGAGCGCATCTACAAGTGCATCATCTGCACGTACACGACGGTCAGCGAGTACCA
  CTGGAGGAAACACCTGAGAAACCATTTCCCCAGGAAAGTCTACACCTGCAGCAAGTGCAACTACTTCTCA
  GACAGAAAAAATAACTACGTTCAGCACGTGCGAACTCACACAGGAGAACGCCCGTATAAATGTGAACTTT
  GTCCTTACTCAAGCTCTCAGAAGACTCATCTAACGCGACACATGCGGACTCATTCAGGTGAGAAGCCATT
  TAAATGTGATCAGTGCAATTATGTGGCCTCTAATCAGCATGAAGTGACCCGACATGCAAGACAGGTTCAC
  AACGGGCCTAAACCTCTTAATTGCCCGCACTGTGACTACAAAACAGCAGATAGAAGCAACTTCAAAAAGC
  ACGTGGAGCTGCATGTTAACCCACGGCAGTTCAACTGCCCCGTGTGTGACTACGCGGCTTCTAAGAAGTG
  TAATCTACAATACCATTTCAAATCTAAGCATCCCACCTGTCCCAGCAAAACAATGGATGTCTCCAAAGTG
  AAGCTAAAGAAAACCAAAAAGAGAGAGGCTGACCTGCTTAATAACGCCGTCAGCAACGAGAAGATGGAGA
  ATGAGCAAACAAAAACAAAGGGGGATGTGTCTGGGAAGAAGAACGAGAAACCTGTAAAAGCTGTGGGAAA
  AGATGCTTCAAAAGAGAAGAAGCCTGGTAGCAGTGTCTCAGTGGTCCAGGTAACTACCAGGACTCGGAAG
  TCAGCGGTGGCGGCGGAGACTAAAGCAGCAGAGGTGAAACACACAGACGGACAAACAGGAAACAATCCAG
  AAAAGCCCTGTAAAGCCAAGAAAAACAAAAGAAAGAAGGATGCTGAGGCCCATCCCTCCGAAGAGCCTGT
  GAACGAGGGACCAGTGACAAAAAAGAAAAAGAAGTCTGAGTGCAAATCAAAAATCGGTACCAACGTGCCA
  AAGGGCGGCGGCCGAGCGGAGGAGAGGCCGGGGGTCAAGAAGCAAAGCGCTTCCCTTAAGAAAGGCACAA
  AGAAGACGCCGCCCAAGACAAAGACAAGTAAAAAAGGTGGCAAACTTGCTCCAAAGGGGATGGGGCAGAC
  AGAACCTTCTTCTGGGGCATTGGCTCAAGTGGGGGTGTCTCCAGACCCTGCCCTCATTCAGGCAGAGGTC
  ACCGGGTCAGGATCTTCTCAGACAGAGCTTCCTTCACCCATGGATATTGCTAAGTCAGAGCCCGCCCAGA
  TGGAGGTTTCCCTAACAGGGCCACCTCCGGTGGAGCCTGCTCAAATGGAGCCATCGCCTGCGAAACCTCC
  CCAGGTAGAAGCACCCACTTACCCCCAGCCTCCCCAAAGGGGGCCTGCCCCTCCCACGGGGCCTGCCCCT
  CCCACGGGGCCTGCCCCTCCCACGGAGCCTGCCCCTCCCACGGGGCTTGCCGAGATGGAACCTTCTCCCA
  CGGAGCCTTCCCAGAAGGAACCACCTCCCAGTATGGAGCCTCCCTGCCCCGAGGAGCTGCCTCAGGCCGA
  GCCACCTCCTATGGAGGATTGTCAGAAGGAGCTGCCTTCTCCCGTGGAGCCCGCTCAGATTGAGGTTGCT
  CAGACGGCCCCTACGCAGGTTCAGGAGGAGCCCCCTCCTGTCTCGGAGCCACCTCGGGTGAAGCCAACCA
  AAAGATCATCTCTCCGGAAAGACAGAGCAGAGAAGGAGCTGAGCCTGCTGAGTGAGATGGCGCGGCAGGA
  GCAGGTCCTCATGGGGGTTGGCTTGGTGCCTGTTAGAGACAGCAAGCTTCTGAAGGGAAACAAGAGCGCC
  CAGGACCCCCCAGCCCCACCGTCACCATCGCCAAAGGGAAACTCGAGGGAAGAGACACCCAAGGACCAAG
  AAATGGTCTCTGATGGGGAAGGAACTATAGTATTCCCTCTCAAGAAAGGAGGACCAGAGGAAGCTGGAGA
  GAGTCCAGCTGAGTTGGCTGCTCTCAAGGAGTCTGCCCGTGTTTCATCCTCTGAACAAAACTCAGCCATG
  CCAGAGGGTGGAGCATCACACAGCAAGTGTCAGACTGGCTCCTCTGGGCTTTGTGACGTGGACACTGAGC
  AGAAGACAGATACTGTCCCCATGAAAGACTCCGCAGCAGAGCCAGTGTCCCCTCCTACCCCAACAGTGGA
  CCGTGACGCAGGGTCACCAGCTGTAGTGGCCTCCCCTCCTATCACGTTGGCTGAAAACGAGTCTCAGGAA
  ATTGATGAAGATGAAGGCATCCATAGCCATGATGGAAGTGACCTGAGTGACAACATGTCTGAGGGGAGTG
  ACGACTCAGGACTGCACGGGGCTCGGCCGACACCACCAGAAGCTACGTCAAAAAATGGGAAGGCAGGGTT
  GGCTGGTAAAGTGACTGAGGGAGAGTTTGTGTGTATTTTCTGTGATCGTTCTTTTAGAAAGGAAAAAGAT
  TATAGCAAACACCTCAATCGCCACTTGGTGAATGTGTACTTCCTAGAAGAAGCAGCTGAGGAGCAGGAGG
  AGCAGGAGGAGCGGGAGGAGCAGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011263
• Insert Size: 3249 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011263.2, NP_035393.2
• RefSeq Size: 4266 bp
• RefSeq ORF: 3249 bp
• Locus ID: 19712
• UniProt ID: Q8VIG1
• Cytogenetics: 5 C3.3
• Gene Summary: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells (PubMed:29961578, PubMed:9771705). Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes (By similarity). Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier (By similarity). Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression (By similarity). Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 2 (PubMed:21884984). Repressor activity may be inhibited by forming heterodimers with isoform 2, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes (PubMed:10490617, PubMed:11039732). Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes (PubMed:15907476). Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons (PubMed:27819263). Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits (By similarity). Acts as a regulator of osteoblast differentiation (PubMed:25727884). Key repressor of gene expression in hypoxia; represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions (By similarity). May also function in stress resistance in the brain during aging; possibly by regulating expression of genes involved in cell death and in the stress response (PubMed:24670762). Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death (By similarity). After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death (By similarity).[UniProtKB/Swiss-Prot Function]