Ap3b2 (NM_021492) Mouse Untagged Clone

CAT#: MC223471

Ap3b2 (untagged) - Mouse adaptor-related protein complex 3, beta 2 subunit (Ap3b2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ap3b2
• Synonyms: AI549966; AU042881; beta-NAP; beta3B; Naptb; [b]-NAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223471 representing NM_021492
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGCTCCGGCCTACAGCGAAGACAAGGGCGGCTCAGCCGGTCCCGGGGAGCCAGAATATGGCC
  ACGACCCGGCGAGCGGCGGTATCTTCTCCTCGGACTACAAGCGGCATGATGACCTGAAAGAGATGTTGGA
  CACCAACAAGGACTCCCTCAAGCTGGAGGCCATGAAGAGGATTGTGGCAATGATCGCCCGGGGAAAGAAT
  GCCTCGGACCTGTTCCCTGCAGTGGTGAAGAATGTGGCCTGTAAGAACATAGAGGTGAAGAAGCTTGTCT
  ACGTGTATCTGGTCCGATATGCCGAGGAGCAGCAAGACCTCGCTCTACTGTCCATCTCTACTTTCCAACG
  TGGCCTAAAGGATCCCAACCAGCTGATCCGTGCCAGTGCGCTCCGGGTTCTCTCTAGCATCCGTGTGCCC
  ATCATAGTGCCCATCATGATGCTAGCCATCAAGGAAGCTGCCTCGGACATGTCACCCTACGTTCGGAAAA
  CAGCTGCTCATGCCATCCCCAAACTCTACAGTTTGGACTCCGATCAGAAGGACCAGCTGATAGAAGTCAT
  TGAGAAGCTTTTGGCAGATAAGACCACGCTGGTGGCAGGCAGTGTAGTGATGGCCTTTGAAGAGGTATGT
  CCAGAACGTATTGACCTGATTCACAAAAACTACCGGAAGCTCTGCAACCTGCTCATTGATGTGGAGGAGT
  GGGGCCAGGTGGTGATTATCAGCATGCTCACGCGTTATGCGCGAACGCAATTCCTGAGCCCTACGCAGAA
  TGAGTCCTTGCTGGAGGAGAATCCCGAGAAGGCCTTCTATGGCTCCGAGGAGGACGAGGCCAAAGGCCCT
  GGATCAGAGGAGGCAGCCACTGCGGCGCTGCCCGCTCGGAAGCCCTATGTAATGGACCCAGATCACCGGC
  TGCTGCTGCGGAACACCAAGCCCCTGCTGCAGAGCCGCAGCGCCGCGGTGGTCATGGCGGTGGCGCAGCT
  TTACTTCCACCTAGCGCCTAAGGCAGAGGTGGGCGTCATCGCCAAGGCGCTGGTACGCCTGCTGCGCAGC
  CACAGTGAGGTGCAGTACGTAGTGCTCCAGAACGTGGCCACCATGTCCATCAAGCGCAGGGGTATGTTTG
  AACCCTACCTGAAGAGCTTCTACATTAGGTCCACGGATCCTACCCAGATCAAGATCCTGAAGCTGGAAGT
  GCTGACCAACCTGGCCAATGAGACTAACATCCCCACTGTTCTCCGGGAATTCCAGACCTACATTCGCAGC
  ATGGACAAAGACTTTGTGGCAGCCACGATCCAGGCCATTGGACGATGTGCAACGAACATAGGCCGAGTCC
  GTGACACCTGTCTCAATGGCTTGGTGCAGCTTCTGTCCAACCGTGACGAGCTTGTGGTTGCAGAGTCAGT
  GGTGGTCATTAAGAAGCTGCTGCAAATGCAGCCAGCGCAGCACGGTGAGATCATCAAACACTTGGCAAAG
  CTCACAGACAACATCCAGGTGCCCATGGCACGAGCCAGCATCCTCTGGCTCATTGGAGAGTACTGTGAGC
  ATGTCCCCAAGATCGCACCTGATGTCCTGAGAAAAATGGCCAAGTCCTTCACAGCAGAGGAGGACATCGT
  GAAGCTGCAGGTCATCAACCTGGCAGCCAAGCTGTACCTGACTAACTCAAAGCAGACCAAGCTCTTGACC
  CAGTATGTGCTGAGTCTGGCCAAGTACGACCAGAACTATGATATCCGGGATCGAGCCCGCTTCACCCGGC
  AGCTCATTGTTCCCTCTGAGCAGGGTGGTGCCCTCAGTCGCCATGCCAAGAAACTCTTCCTGGCCCCTAA
  GCCAGCCCCTATCTTGGAGTCATCCTTCAAAGATCGTGACCATTTCCAGCTGGGCTCACTGTCACACCTG
  CTCAATGCAAAGGCTACAGGCTACCAGGAGCTCCCGGACTGGCCAGAGGAAGCCCCAGATCCATCTGTGC
  GCAATGTGGAGGTACCTGAATGGACCAAATGTTCAAATCGGGAGAAGAGGAAGGAGAAGGAAAAGCCTTT
  CTATTCTGACTCCGAGGGGGAGTCTGGCCCCACTGAGTCTGCAGACAGCGAGCCTGAATCTGAGAGTGAG
  TCAGAGAGTAAGAGCAGCAGTGGGAGTGGCTCCGGGGAGTCTAGCAGTGAGTCGGACAATGAAGAAGAGG
  ATGAGGAGAAAGGCGGAGGCAGCGAGAGTGAACAGAGTGAGGAGGAAGATGAGAAGAAGAAGAAGACAAA
  GAAGAAGAAGGCATCAGAGGGACACAGAGAAGGCTCATCATCAGAAGAGGGCAGTGACTCCAGCAGCAGC
  TCAGAGTCTGAGGTGACGTCAGAGTCGGAGGAGGAACAGGTGGAACCTGCCTCCTGGAGGAAGAAAACGC
  CTCCAGGCAGCAAGAGTGCCCCTGTAGCCAAGGAGATTTCCCTGCTGGACCTAGAGGATTTCACCCCTCC
  TAGTGTCCAGCCTGTGTCTCCTCCCATGGTTGTGTCTACCAGTCTGGCTGCTGACCTGGAAGGGCTGACA
  CTCACAGACTCCTCTCTGGTGCCTTCGCTGCTGAGTCCAGTGTCCAGCATTGGGAGGCAGGAGCTGCTGC
  ACCGGGTAGCTGGAGAGGGGCTGTCTGTGGACTATGCCTTCAGCCGCCAGCCTTTCTCTGGGGATCCCCA
  CATGGTGTCTCTGCATATCTACTTCTCCAACAACTCGGAGACTCCCATCAAGGGGCTGCATGTGGGCACT
  CCCAAGCTGCCTGCTGGCATCAGCATTCAAGAATTCCCTGAAATCGAGTCCCTGGCACCTGGAGAATCCA
  CCACTACTGTGATGGGCATTAACTTCTGCGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAAAC
  CCGACAGTTCTATGTCTCCATTCAGCCACCTGTCGGGGAGCTGATGGCCCCTGTATTTATGAGTGAGAAT
  GAGTTCAAGAAGGAACAGGGAAAGCTGACAGGCATGAATGAGATCACAGAGAAGCTTACATTGCCAGACA
  CCTGTCGAAGTGATCACATGGTGGTGCAGAAAGTGACTGCCACCGCCAACCTAGGTCGTGTCCCATGTGG
  GACATCTGATGAGTACAGGTTTGCAGGGAGGACATTAACTAGTGGGAGTCTGGTCCTGCTGACTCTGGAT
  GCCCGGGCAGCTGGAGCCGCCCAGCTGACCGTCAACAGTGAGAAGATGGTGATTGGCACCATGCTGGTGA
  AGGATGTGATTCAGGCTCTGACCCAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_021492
• Insert Size: 3249 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021492.3, NP_067467.2
• RefSeq Size: 3816 bp
• RefSeq ORF: 3249 bp
• Locus ID: 11775
• UniProt ID: Q9JME5
• Cytogenetics: 7 45.71 cM
• Gene Summary: Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.[UniProtKB/Swiss-Prot Function]