Larp1 (NM_028451) Mouse Untagged Clone

CAT#: MC223454

Larp1 (untagged) - Mouse La ribonucleoprotein domain family, member 1 (Larp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Larp1
• Synonyms: 1810024J12Rik; 3110040D16Rik; Larp; mKIAA0731
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223454 representing NM_028451
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTCAAGTGGAGCCGCTGCTGCCGGCGGGCGCCCCGCTGCTGCAGGCCGAGGAGCACGGCCTGG
  CGAGGAAGAAGCCGGCGCCCGACGCCCAGGCCGAGTCGGGCCCGGGTGACGGCGGCGGAGAGCCCGACGG
  CGGCGTCCGCAGGCCGCGGCCCGCCTGCGCCAGGCCTGGCCGCGACGGCGCGGAGCGCGAGAGCCCGCGG
  CCACCCGCAGCTGCGGAAGCCCCAGCGGGGAGCGACGGCGAGGACGGCGGCCGGCGTGACTTCGTGGAGG
  CACCGCCGCCCAAGGTGAACCCGTGGACGAAGCACGCGCCGCCGCCGGCCGCGGTGAACGGACAGCCGCC
  TCCAGAACCTTCTGCTCCAGCCAAGGTGGTGAGGGCAGCTGCTCCTAAACCGCGTAAGGGCAGCAAGGTT
  GGTGATTTTGGAGATGCAGTCAACTGGCCTACACCTGGAGAGATAGCCCACAAAAGTGTGCAGCCACAGT
  CCCACAAGCCTCAGCCTGCTCGTAAGTTGCCACCTAAGAAGGACATGAAGGAGCAAGAGAAAGGAGATGG
  AAGTGATAGTAAGGAGAGCCCAAAAACAAAGTCGGATGAATCGGGGGAGGAAAAGAATGGGGATGAGGAC
  TGCCAGCGAGGCGGGCAGAAGAAGAAAGGGAGCAAACACAAGTGGGTTCCATTGCAAATAGACATGAAGC
  CCGAAGTACCCAGAGAGAAATTGGCCTCCCGTCCCACTCGCCCACAGGAACCAAGACATACACCCGCTGT
  CCGTGGGGAGATGAAAGGATCTGAACCTGCCACCTACATGCCTGTATCTGTGGCCCCACCCACCCCAGCC
  TGGCAACCAGAGACCAAAGTGGAGCCTGCCTGGCACGACCAGGATGAGACATCGAGTGTGAAGAGTGATG
  GGGCTGGTGGGGCTCGGGCTTCCTTCCGTGGCCGTGGACGGGGGCGTGGTCGTGGACGGGGCCGTGGACG
  GGGTGGCACTCGAACCCACTTTGACTACCAGTTTGGCTACCGAAAGTTTGATGGTACAGAGGGGCCCCGC
  ACCCACAAGTATATGAACAACATCACTTACTACTTTGACAATGTCAGCAGCAATGAGATTTACAGTATGG
  ACCAGGAGCTGCTCAAGGATTATATCAAACGCCAGATTGAATATTACTTCAGCGTGGACAATTTAGAGCG
  AGACTTCTTCCTGAGACGGAAAATGGATGCTGATGGCTTCCTTCCCATCACCCTTATTGCTTCCTTTCAC
  CGTGTACAGGCCCTAACCACTGACATTTCACTCATCTTTGCGGCTCTAAAGGACAGCAAGGTGGTAGAGA
  TGGTTGAGGAGAAAGTCCGCAGGAGGGAAGAACCTGAAAAGTGGCCACTGCCTGGACCCCCAATAGTGGA
  TTATTCACAAACTGATTTTTCTCAGCTTCTCAACTGCCCAGAGTTTGTTCCCCGCCAGCACTACCAGAAA
  GAGACAGAGTCGGCACCTGGCTCTCCCCGTGCAGTCACCCCAGTGCCAACAAAAACAGAGGAGGTCAGCA
  ACCTGAAGACCCTTCCCAAGGGCCTGTCTGCCAGCCTCCCAGATCTGGATTCTGAGAGCTGGATTGAAGT
  GAAGAAAAGGCCTCGACCCTCACCAGCACGCCCCAAGAAGCCAGAGGAGCCCAGGTTCTCCCACCCAACT
  GCTCTGCCTCAGCAGCTTCCCTCCCAGCAGCTGATGTCTAAGGATCAGGATGAGCAAGAAGAACTAGACT
  TTCTGTTTGACGAGGAGATGGAGCAGATGGATGGGCGGAAGAATACCTTCACTGCCTGGTCTGAAGAGGA
  CTCTGACTATGAAATTGATGACCGGGATGTCAATAAGATCCTCATCGTCACTCAAACACCACCTTACATG
  CGTCGCCACCCTGGAGGAGACCGTACAGGCAACCACACTTCGCGTGCCAAGATGAGTGCTGAACTGGCCA
  AGGTCATTAATGATGGACTCTTCTACTATGAACAAGACCTGTGGACTGAGAAGTTTGAGCCTGAGTATTC
  ACAGATCAAGCAAGAAGTGGAAAACTTCAAGAAAGTGAATATGATTAGTCGGGAACAGTTTGACACTCTG
  ACCCCTGAGCCCCCTGTGGATCCTAACCAGGAGGTTCCTCCTGGGCCACCTCGGTTCCAACAAGTTCCTA
  CTGATGCCCTGGCCAACAAGCTGTTTGGTGCTCCCGAGCCATCTACCATTGCCCGTTCTCTACCAACCAC
  TGTCCCAGAGTCTCCAAACTACCGAAATGCCAGGACTCCCCGCACACCCCGGACCCCCCAGCTCAAAGAC
  TCTAGTCAGACACCACGGTTTTACCCTGTGGTGAAAGAAGGACGGACCCTAGATGCCAAGATGCCTCGAA
  AAAGAAAGACAAGGCACAGCTCGAACCCCCCCTTGGAAAGTCATGTGGGCTGGGTGATGGATTCTCGGGA
  GCACAGGCCCAGGACTGCTTCTATTAGCTCCAGTCCTTCAGAGGGAACACCTGCAGTCGGCAGCTATGGC
  TGTACCCCTCAGTCACTGCCCAAGTTCCAGCATCCTTCCCATGAGCTGCTCAAGGAAAATGGCTTCACAC
  AACACGTCTACCACAAGTATCGGAGGCGCTGCCTTAATGAGCGGAAGCGCTTGGGCATTGGCCAGTCTCA
  GGAGATGAACACCCTCTTCCGCTTTTGGTCCTTCTTCCTCCGAGATCACTTCAATAAAAAGATGTATGAA
  GAGTTCAAGCAGCTCGCACTGGAGGATGCCAAAGAAGGCTACAGGTACGGTTTGGAGTGCCTTTTTCGAT
  ACTACAGTTATGGTCTAGAGAAAAAGTTCCGGCTGGACATATTCAAGGATTTCCAGGAGGAGACAGTGAA
  GGACTATGAGGCTGGCCAGCTCTATGGATTGGAGAAGTTCTGGGCCTTCTTGAAATACTCCAAAGCCAAA
  AATTTGGACATTGACCCCAAACTGCAAGAATACCTCGGCAAATTCCGCCGCTTAGAAGACTTCCGAGTGG
  ACCCCCCCATGGGCGAGGAAGGCAACCACAAGCGACACCCAGTGGTAGCAGGAGGCAGCGGAGAGGGCAG
  GAAGCGGTGCCCTTCCCAGTCTTCCAGCAGGCCTGCCACTGGGATCAGCCAACCCCCTACGACACCTACT
  GGCCAGGCCACTCGAGAAGATGCCAAATGGACAAGCCAGCACTCGGACACACTGACTTTGCGAAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028451
• Insert Size: 3219 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028451.1, NP_082727.1
• RefSeq Size: 6617 bp
• RefSeq ORF: 3219 bp
• Locus ID: 73158
• UniProt ID: Q6ZQ58
• Cytogenetics: 11 B1.3
• Gene Summary: RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability. Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery. The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1. When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA. Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif. Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes. Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis. Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation. Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis.[UniProtKB/Swiss-Prot Function]