Itga6 (NM_008397) Mouse Untagged Clone

CAT#: MC223447

Itga6 (untagged) - Mouse integrin alpha 6 (Itga6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itga6
• Synonyms: 5033401O05Rik; AI115430; Cd49f; VLA-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223447 representing NM_008397
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCGCGGGCCAGTTGTGCCTGCTCTACCTGTCCGCGGGGCTTCTAGCCCGGCTGGGTACAGCCT
  TCAACCTGGACACCCGCGAGGACAACGTGATCCGGAAATCGGGGGATCCCGGGAGCCTCTTCGGCTTCTC
  GCTCGCCATGCACTGGCAGTTGCAGCCGGAGGACAAGCGGCTGTTGCTTGTGGGGGCACCTCGGGCAGAA
  GCACTCCCGCTGCAGAGGGCGAACAGAACAGGGGGCCTGTACAGCTGTGACATCACCTCCCGGGGACCTT
  GTACACGGATTGAATTTGATAATGACGCTGATCCTATGTCAGAAAGCAAGGAAGACCAGTGGATGGGAGT
  CACTGTTCAGAGCCAAGGTCCAGGGGGCAAAGTGGTGACGTGTGCACATCGATATGAGAAACGGCAGCAC
  GTCAACACGAAGCAGGAGTCGCGGGATATCTTTGGAAGATGTTATGTCCTAAGTCAGAATCTCAGAATTG
  AAGATGATATGGACGGAGGAGACTGGAGTTTCTGCGATGGCCGGTTGAGAGGCCATGAAAAGTTTGGCTC
  CTGTCAGCAAGGAGTAGCGGCTACTTTCACTAAGGACTTTCATTACATTGTTTTTGGAGCCCCAGGGACT
  TACAACTGGAAAGGGATCGTCCGTGTAGAACAAAAGAATAACACTTTTTTTGACATGAACATCTTTGAAG
  ATGGGCCCTATGAAGTTGGTGGAGAGACTGATCATGATGAAAGTCTCGTGCCCGTTCCTGCTAACAGTTA
  CCTAGGCTTTTCACTGGACTCAGGGAAGGGTATTGTTTCTAAAGATGACATCACTTTTGTGTCTGGTGCT
  CCAAGAGCCAATCACAGTGGGGCTGTAGTTTTGCTAAAAAGAGACATGAAGTCCGCGCATCTGCTCCCTG
  AGTATATATTTGACGGAGAAGGCCTGGCTTCCTCGTTTGGCTATGATGTGGCAGTGGTGGACCTCAATGC
  AGATGGGTGGCAAGACATTGTTATCGGAGCTCCACAGTATTTTGATAGGGATGGTGAAGTCGGGGGTGCA
  GTTTACGTCTACATTAACCAGCAAGGCAAATGGAGTAATGTGAAGCCGATTCGTCTAAATGGGACCAAAG
  ACTCGATGTTTGGAATCTCTGTGAAAAATATAGGTGATATTAACCAAGATGGCTATCCAGATATTGCTGT
  TGGAGCTCCCTATGATGATCTGGGGAAGGTTTTTATCTATCATGGATCCCCGACTGGCATAATTACCAAG
  CCAACACAGGTTCTCGAGGGGACATCGCCTTACTTCGGCTATTCAATCGCTGGGAATATGGACCTGGATC
  GGAATTCCTACCCCGACCTTGCTGTGGGCTCCCTCTCAGACTCGGTCACTATTTTCAGATCCCGGCCAGT
  GATTAACATTCTAAAAACCATCACAGTGACTCCTAACAGAATTGACCTCCGCCAGAAGTCCATGTGTGGC
  TCACCTAGCGGGATATGCCTCAAGGTTAAAGCCTGTTTTGAATATACTGCGAAACCTTCCGGTTATAACC
  CTCCAATATCAATTTTGGGTATTCTCGAAGCTGAAAAAGAAAGAAGAAAGTCAGGGTTGTCATCGAGAGT
  TCAGTTTCGAAACCAAGGTTCCGAGCCAAAGTATACTCAGGAGCTGACCCTGAATCGGCAGAAGCAGCGG
  GCGTGCATGGAGGAGACCCTCTGGCTGCAGGAGAACATCAGAGACAAGCTGCGTCCCATCCCCATCACGG
  CTTCTGTGGAGATCCAGGAGCCCAGCTCTCGCCGGCGGGTGAACTCACTCCCCGAAGTTCTTCCCATCCT
  GAATTCAAATGAAGCCAAAACGGTCCAGACAGATGTCCACTTCTTAAAGGAAGGATGTGGAGACGACAAT
  GTCTGTAACAGCAACCTTAAGCTAGAGTATAAATTTGGTACCCGAGAAGGAAATCAAGACAAATTCTCTT
  ACCTTCCAATTCAAAAAGGCATCCCAGAATTAGTCCTAAAAGATCAGAAAGATATAGCTCTGGAAATAAC
  GGTGACCAACAGCCCTTCGGATCCAAGGAATCCCCGGAAAGATGGCGACGATGCCCATGAAGCCAAACTC
  ATCGCCACGTTTCCAGACACTCTGACATATTCCGCTTACAGAGAACTGAGGGCTTTCCCTGAGAAGCAGC
  TGAGCTGTGTGGCCAACCAGAATGGCTCCCAAGCCGACTGTGAGCTCGGAAATCCTTTCAAGAGAAATTC
  CAGTGTTACTTTCTATCTGATTTTAAGTACAACCGAGGTCACCTTTGACACCACAGATCTGGATATTAAT
  CTGAAGTTGGAAACAACAAGCAATCAGGATAATTTGGCTCCAATTACAGCGAAGGCAAAAGTGGTTATTG
  AATTGCTTTTATCGGTCTCCGGAGTCGCTAAGCCTTCGCAGGTGTATTTTGGAGGTACAGTTGTTGGTGA
  GCAAGCTATGAAATCTGAAGATGAAGTAGGAAGTTTAATAGAGTATGAATTTAGGGTGATTAACTTAGGC
  AAGCCTCTTAAAAACCTCGGCACAGCAACCTTGAATATACAGTGGCCCAAGGAGATTAGCAATGGCAAAT
  GGTTGCTTTATTTGATGAAAGTTGAATCCAAAGGTTTGGAGCAGATTGTTTGTGAGCCACACAATGAAAT
  AAACTACCTGAAGCTGAAGGAGTCTCACAACTCAAGAAAGAAACGGGAACTTCCTGAAAAACAGATAGAT
  GACAGCAGGAAATTTTCTTTATTTCCTGAAAGAAAATACCAGACTCTCAACTGCAGCGTCAACGTGAGGT
  GTGTGAACATCAGGTGCCCACTGCGAGGGCTGGACAGCAAGGCCTCTCTCGTTCTTCGTTCCAGGTTGTG
  GAACAGCACATTTCTAGAGGAATATTCCAAACTGAACTACTTGGACATTCTCCTGAGGGCTTCCATAGAT
  GTCACCGCTGCTGCTCAGAATATCAAGCTCCCTCACGCGGGCACTCAGGTTCGAGTGACGGTGTTTCCCT
  CAAAGACTGTAGCTCAGTATTCAGGAGTAGCTTGGTGGATCATCCTCCTGGCTGTTCTTGCCGGGATTCT
  GATGCTGGCTCTATTAGTGTTTTTACTGTGGAAGTGTGGCTTCTTCAAGAGAAATAAGAAAGATCATTAC
  GATGCCACCTATCACAAGGCTGAGATCCATACTCAGCCGTCTGATAAAGAGAGGCTTACTTCCGATGCAT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008397
• Insert Size: 3222 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008397.4, NP_032423.2
• RefSeq Size: 6018 bp
• RefSeq ORF: 3222 bp
• Locus ID: 16403
• Cytogenetics: 2 42.79 cM
• Gene Summary: This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 4 to bind laminin and to form the main component of hemidesmosomes, which mediate attachment of epithelia to basement membranes. In mouse, deficiency of this gene is associated with absence of hemidesmosomes, severe skin blistering, and early post-natal death. In humans mutations of this gene are associated with epidermolysis bullosa. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.