Srgap2 (NM_001081011) Mouse Untagged Clone

CAT#: MC223445

Srgap2 (untagged) - Mouse SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Srgap2
• Synonyms: 9930124L22Rik; AI448945; FBP2; Fnbp2; srGAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223445 representing NM_001081011
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATCGCAGAGTACGATACTCAGGTCAAAGAGA
  TTAGGGCTCAGCTCACAGAGCAGATGAAATGCCTAGATCAGCAATGTGAGCTCCGGGTGCAGCTGTTGCA
  GGACCTGCAGGACTTCTTCCGCAAGAAGGCTGAGATTGAAATGGACTACTCTCGAAACCTGGAGAAGCTA
  GCCGAGCGCTTTCTAGCCAAGACACGAAGCACCAAGGACCAGCAATTTAAGAAGGACCAGAATGTTCTTT
  CTCCAGTCAACTGCTGGAACCTCCTCTTAAACCAAGTGAAGCGGGAGAGCAGGGATCATACCACCCTGAG
  TGACATCTACCTGAATAATATAATTCCTCGATTTGTTCAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA
  AAGAGTAAAGAAGTTGGCCAGCAACTCCAAGATGATTTGATGAAAGTCCTGAACGAGCTTTACTCGGTCA
  TGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTGAAGGAAGCAGAGAAACA
  AGAAGAGAAGCAAATTGGCAAATCAGTAAAGCAAGAGGACCGGCAGACCCCTCGCTCCCCTGACTCCACA
  GCCAATGTCCGCATTGAGGAGAAGCATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGA
  AGCGACAAGCCAAGTACACAGAAAATAAGCTGAAGGCCATTAAAGCCCGGAATGAGTATTTATTGGCTTT
  GGAGGCAACCAATGCATCTGTCTTCAAGTACTATATCCATGACCTGTCTGATATTATTGATCAATGTTGT
  GACCTAGGCTACCATGCTAGCCTGAACCGGGCTCTACGCACTTTCCTATCTGCTGAATTAAATCTGGAAC
  AGTCAAAACATGAAGGTCTGGATGCTATTGAAAATGCAGTAGAAAACCTAGATGCCACCAGTGACAAGCA
  ACGGCTCATGGAGATGTACAACAATGTTTTTTGCCCCCCTATGAAATTTGAATTCCAGCCCCACATGGGA
  GATATGGCCTCTCAACTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTGCAGAGATGCCAACAGCTGC
  AGTCTCGCTTATCCACTTTGAAGATTGAGAATGAAGAGGTGAAAAAGACAATGGAGGCCACCCTGCAGAC
  CATTCAAGACATTGTGACTGTTGAGGATTTTGATGTATCTGACTGCTTCCAGTATAGCAATTCCATGGAG
  TCTGTCAAATCAACTGTCTCTGAAACATTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCGAACCAGC
  AAGAGACGGAACAGTTTTATTTCACGAAAATGAAGGAGTACTTGGAGGGTAGGAACCTCATCACAAAGCT
  ACAAGCCAAGCATGACCTCCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGACTGTAGTCTTGCCAGG
  CGAAGCTCAACTGTGAGGAAACAGGATTCCAGCCAAGCAATTCCTCTGGTGGTAGAAAGCTGCATCCGGT
  TTATTAGCAGACATGGCCTACAACATGAGGGAATTTTCCGGGTTTCTGGATCACAAGTAGAAGTGAACGA
  CATAAAAAACGCCTTTGAGAGAGGAGAGGATCCCCTGGCTGGGGACCAGAATGACCATGACATGGACTCT
  ATAGCTGGTGTCCTCAAGCTTTACTTCCGGGGCCTGGAACACCCGCTCTTCCCTAAAGACATCTTCCATG
  ACTTGATTGCCTGTGTCACAATGGACAACCTGCAAGAGAGAGCTGTGCATATCCGGAAAGTCCTTCTGGT
  CCTGCCCAAGCCCACTCTGATTATCATGAGATATCTCTTTGCCTTCCTCAATCACTTATCACAGTTCAGT
  GAAGAGAACATGATGGACCCCTACAACCTTGCCATCTGCTTCGGGCCCTCACTGATGTCAGTGCCAGAGG
  GCCACGACCAGGTGTCCTGTCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAACATGAGAA
  CATTTTCCCAAACCCCAGGGAGCTGGAGGGTCCCATCTACAGCAGAGGAGGAAGCATGGAGGATTACTGT
  GACAGCACTCATGGAGAGACTACCTCTGCTGAAGACTCCACCCAGGACGTTACAGCGGAGCACCACACAA
  GCGATGATGAATGTGAGCCCATAGAAGCCATTGCCAAGTTTGACTACGTAGGCCGGACAGCCCGAGAACT
  GTCTTTCAAGAAGGGAGCATCCCTGCTGCTCTACCAGCGAGCTTCTGATGACTGGTGGGAGGGCCGGCAC
  AACGGTATAGATGGACTCATCCCCCATCAGTACATCGTAGTCCAAGACACCGAGGACGGTGTCGTGGAGA
  GGTCCAGCCCCAAGTCTGAGATTGAGGTCATGTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAACGGG
  GGCCAGCTGTCCCAGTGGGGGTCATGTAGCTGATATTTATCTTGCAAACATCAACAAGCAAAGGAAGCGT
  CCAGAATCTGGGAGCATCAGAAAAGCATTTCGGAGTGACAGCCATGGGCTGGGCAGTTCTCTGACTGACT
  CCTCCTCCCTGGGGGTGGGGGCTAGCTGCCGTCCATCCTCCCAGCCCATCATGAGCCAGAATCTCCCCAA
  GGAAGGGCCAGATAAGTGTTCCATCAGCGGCCATGGCAGCCTCAACTCTATCAGCCGCCACTCATCCTTG
  AAGAACCGGATGGACAGTCCGCAGATCCGGAAGACCGCTACGGCAGGAAGGTCAAAAAGTTTCAATAACC
  ATCGGCCCATGGACCCTGAAGTCATTGCACAGGATATTGAAGCAACAATGAACTCTGCCCTGAATGAGCT
  TCAAGAGCTAGAGCGGCAGAGCAGTGCTAAGCACACACCTGATGTGGTTCTGGACACCTTGGAACCACTC
  AAGACCTCCCCGGTGGTAGCCCCCACATCTGAGCCCTCCAGCCCTCTGCACACCCAGCTCCTCAAGGACC
  CTGAGCCTGCCTTCCAGCGCAGCGCTAGTACTGCTGGGGACATTGCCTGCGCCTTCCGGCCTGTAAAGTC
  TGTCAAGATGGCTGCTCCAGTCAAACCACCAGCCACACGGCCCAAGCCAACTGTCTTCCCCAAAACAAAC
  GCCACTAGCCCTGGTGTCAATTCATCTGCTTCCCCACAGGCCACTGACAAGTCTTGTACTGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081011
• Insert Size: 3216 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081011.2, NP_001074480.2
• RefSeq Size: 8040 bp
• RefSeq ORF: 3216 bp
• Locus ID: 14270
• UniProt ID: Q91Z67
• Cytogenetics: 1 E4
• Gene Summary: RAC1 GTPase activating protein (GAP) that binds and deforms membranes, and regulates actin dynamics to regulate cell migration and differentiation. Plays an important role in different aspects of neuronal morphogenesis and migration mainly during development of the cerebral cortex. This includes the biogenesis of neurites, where it is required for both axons and dendrites outgrowth, and the maturation of the dendritic spines. Also stimulates the branching of the leading process and negatively regulates neuron radial migration in the cerebral cortex. May play a role for cognition, learning and memory. In non-neuronal cells, it may also play a role in cell migration by regulating the formation of lamellipodia and filopodia.[UniProtKB/Swiss-Prot Function]