Pum2 (NM_001160220) Mouse Untagged Clone

CAT#: MC223420

Pum2 (untagged) - Mouse pumilio 2 (Drosophila) (Pum2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pum2
• Synonyms: 5730503J23Rik; Pumm2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223420 representing NM_001160220
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT
  TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA
  GACTGCATGGGGAACTTCTCATCATTCAATGTCCCAGCCTATTATGGTGCAGAGAAGATCTGGACAGAGT
  TTTCATGGAAACAGTGAAGTAAATGCAATTCTTTCTCCACGCTCAGAAAGTGGAGGCCTTGGTGTGAGCA
  TGGTAGAATATGTATTAAGTTCCTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAACTTTTGG
  TACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG
  GACCAAAACAGAGATCTTAAACAAGATGATGAGGACTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAA
  TGGATGCCGATTGCAAAGATTTTAATCGCACTCCTGGAAGTCGCCAAGCCTCTCCAACTGAAGTAGTTGA
  GCGCCTTGGCCCTAGTACTAATCCCCCAGAAGGATTGGGCCCTCTTCCTAATCCGACAGCGAATAAACCA
  CTTGTTGAAGAATTTTCAAACCCTGAAACTCAGAATCTGGATGCAATGGACCAAGTTGGTCTGGATTCCT
  TACAGTTTGACTATCCTGGTAATCAGGTACCCATGGATTCTTCAGGAGCTACTGTAGGCCTTTTTGACTA
  CAATTCCCAACAGCAGCTCTTTCAGAGGACTAGTGCACTAACAGTTCAGCAGTTAACTGCAGCTCAGCAG
  CAGCAGTATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTCCAG
  CTGCATTTGTGCCAAATCCATATATTATTAGTGCTGCTCCTCCAGGGACTGACCCGTATACTGCAGCAGG
  ATTGGCTGCAGCAGCAACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGTGTTCCATGGGGA
  GTGTATCCAGCCAATTTATTTCAGCAACAAGCTGCAGCTGCGGCAAGCAACACAGCCAACCAGCAAGCAG
  CATCACAAGCTCAGCCTGGACAGCAGCAGGTTCTTCGTCCTGGAGCAGGTCAGCGTCCTATTACTCCAAG
  TCAGGGCCAACAAGGGCAGCAAGCAGAGTCACTTGCAGCAGCTGCAAACCCAACTTTGGCTTTTGGTCAG
  AGTCTTGCTGCAGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCT
  TAGTGGTTGGCCCCGGAGCAAGAACTGGCCTTGGAGCTCCAGTACGATTAATGGCTCCAACACCTGTCTT
  AATAAGTTCAACAGCAGCACAAGCTGCAGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAATAGTCTTACA
  GGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAGCAGCAGCAGCAAC
  CAAGCACTAACCTGCAGTCTAATTCATTTTATGGGAGCAGCTCTTTGACTAACAGCTCCCAGAGCAGCTC
  TTTATTCTCTCATGGACCTGGCCAACCTGGAAGTGCGTCTCTTGGCTTTGGAAGTGGTAGCTCTTTAGGA
  GCTGCTATAGGCTCAGCTCTCAGTGGATTTGGCTCATCAGTTGGCAGTTCTGCAAGTAGTAGTGCCACAA
  GGAGAGAGTCTCTATCTACTAGCTCTGACTTGTACAAAAGATCTAGTAGCAGCCTAGCACCCATAGGGCA
  ACCATTTTACAATAGTCTGGGATTTTCCTCCTCTCCAAGTCCAATAGGCATGCCTCTGCCAAGCCAAACT
  CCAGGACATTCACTTACGCCACCGCCATCACTTTCATCACATGGATCCTCATCCAGTTTGCATTTAGGAG
  GACTGACAAACGGTAGTGGTCGGTATATCTCTGCAGCACCTGGAGCAGAAGCAAAGTACCGAAGTGCTTC
  AAGCACTTCCAGTCTATTTAGCTCCAGCAGCCAGCTCTTTCCTCCTTCTCGGCTCCGCTATAATAGATCT
  GACATCATGCCCTCCGGCCGCAGTAGGTTATTGGAAGATTTCAGGAACAACCGCTTCCCCAACCTTCAGC
  TCAGAGACTTAATTGGACATATAGTCGAGTTTTCTCAAGACCAGCATGGTTCCAGATTCATACAGCAAAA
  GCTAGAGAGAGCTACTCCAGCTGAGCGACAAATAGTATTTAATGAAATTCTACAGGCAGCCTATCAATTA
  ATGACAGATGTTTTTGGAAACTATGTTATACAGAAGTTTTTTGAGTTTGGAAGTTTGGATCAGAAATTAG
  CCCTGGCTACTCGTATTCGTGGTCATGTTCTACCATTAGCCTTGCAGATGTATGGCTGCCGTGTTATTCA
  AAAGGCGTTAGAATCTATTTCTTCTGATCAGCAGAGTGAAATGGTTAAGGAACTAGATGGCCATGTACTT
  AAATGTGTGAAAGATCAAAATGGAAACCATGTTGTACAGAAATGCATTGAATGTGTTCAGCCACAGTCAC
  TGCAGTTCATCATCGATGCTTTCAAAGGACAAGTATTTGTGCTTTCAACCCATCCTTATGGCTGCAGAGT
  CATTCAGCGTATCTTAGAGCACTGCACGGCAGAGCAGACCTTACCCATCTTAGAAGAACTTCACCAACAC
  ACAGAACAGTTGGTACAGGATCAGTATGGCAATTATGTTATTCAGCATGTACTGGAACATGGTCGACCTG
  AAGACAAGAGCAAAATTGTTTCCGAAATCAGAGGAAAGGTCTTAGCCCTGAGTCAACACAAATTTGCCAG
  CAATGTAGTAGAAAAGTGTGTTACTCATGCCTCCCGTGCTGAGAGAGCTTTACTGATTGATGAGGTCTGC
  TGTCAGAATGATGGTCCTCACAGTGCCTTATACACCATGATGAAGGATCAGTATGCCAACTATGTGGTTC
  AGAAGATGATTGATATGGCTGAGCCTGCGCAGAGAAAGATAATCATGCACAAGATTCGACCTCATATTAC
  TACTCTTCGCAAATACACATATGGGAAGCATATACTGGCCAAGTTGGAAAAATACTATCTGAAAAACAGC
  CCAGATCTAGGGCCAATTGGAGGACCACCAAATGGGATGCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001160220
• Insert Size: 3195 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160220.1, NP_001153692.1
• RefSeq Size: 6305 bp
• RefSeq ORF: 3195 bp
• Locus ID: 80913
• Cytogenetics: 12 A1.1
• Gene Summary: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3' UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3' UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame donor splice site in the 3' coding region compared to variant 1. This results in an isoform (2) lacking 2 aa compared to isoform 1. Variants 3 and 4 encode the same isoform.