Vcl (NM_009502) Mouse Untagged Clone

CAT#: MC223409

Vcl (untagged) - Mouse vinculin (Vcl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vcl
• Synonyms: 9430097D22; AA571387; AI462105; AW545629
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223409 representing NM_009502
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGTTTCACACGCGTACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCGCACCTGG
  TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTAGCCGCCGT
  GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAAAAGAGACTGTTCAGACCACTGAGGATCAGATTCTG
  AAGAGAGATATGCCACCAGCCTTTATTAAGGTTGAAAATGCTTGCACCAAGCTTGTTCAGGCAGCCCAGA
  TGCTTCAGTCAGACCCATACTCGGTTCCTGCGCGGGATTACCTCATTGACGGCTCTAGGGGAATCCTTTC
  TGGCACATCTGACCTACTGCTTACCTTCGATGAGGCTGAGGTTCGTAAAATTATTAGGGTTTGCAAAGGA
  ATTTTGGAATATCTTACAGTGGCAGAGGTAGTGGAAACTATGGAAGACTTGGTCACTTACACAAAGAATC
  TTGGGCCAGGAATGACTAAGATGGCCAAAATGATTGATGAGAGACAGCAGGAGTTGACTCACCAGGAACA
  CCGTGTGATGTTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG
  ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTTTGAAAAATCGAAATTTTACTG
  TAGAAAAGATGAGTGCTGAAATTAACGAGATCATTCGTGTGTTACAACTCACTTCCTGGGATGAAGATGC
  CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCGTCCATAGACTCCAAATTGAACCAGGCC
  AAAGGTTGGCTCCGTGACCCCAATGCCTCCCCAGGGGATGCTGGAGAGCAGGCCATCAGGCAGATCTTAG
  ATGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAAAT
  GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCTTCCCCAGTGGCCATG
  CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACCGCCAAAGTGGAGAATGCAGCTCGGAAGC
  TGGAAGCCATGACGAACTCAAAGCAGAGCATTGCAAAGAAGATTGATGCTGCCCAGAATTGGCTGGCGGA
  TCCAAATGGTGGACCTGAGGGAGAAGAACAGATTCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCAGAA
  TTATGTGATGATCCTAAGGAGAGAGATGACATCCTCCGCTCCCTTGGAGAGATAGCTGCTCTGACCTCTA
  AACTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCGCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC
  GACGGCACTACAGAACCTGCAGACCAAAACCAACAGGGCCGTGGCCAACAGCAGACCTGCCAAAGCAGCT
  GTCCACCTCGAGGGCAAGATTGAACAGGCGCAGCGGTGGATTGATAACCCCACAGTAGATGACCGTGGAG
  TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAGGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA
  TCGCCAAGATCTTCTTGCCAAATGTGACCGTGTAGACCAGCTAACAGCTCAGCTGGCTGACCTGGCTGCC
  CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCACTTGCATCCCAGCTTCAGGACTCCTTAAAGGATCTTA
  AAGCCCAGATGCAGGAAGCTATGACTCAAGAGGTATCCGATGTTTTCAGCGATACTACAACTCCTATCAA
  GCTGTTGGCAGTAGCCGCCACTGCTCCTCCTGATGCACCCAATAGGGAAGAGGTATTTGATGAAAGGGCA
  GCCAATTTTGAAAACCATTCAGGAAGGCTTGGAGCCACAGCAGAGAAGGCGGCTGCTGTTGGTACTGCCA
  ATAAATCGACAGTGGAAGGCATTCAGGCATCTGTGAAGACAGCCCGAGAACTCACTCCCCAGGTCATCTC
  CGCTGCTCGGATCTTACTGAGGAACCCTGGTAACCAGGCTGCTTATGAACATTTTGAGACCATGAAGAAC
  CAGTGGATTGATAATGTTGAAAAAATGACAGGGCTGGTGGACGAGGCTATTGATACCAAGTCTCTGTTGG
  ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA
  GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG
  GTAGAGAACTCTGAGGACCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA
  TCTCCCCCATGGTGATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT
  CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAGGTCAGAGAAGCCTTCCAACCTCAGGAGCCT
  GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGACTAACTGATGAGCTGGCTCCTCCTAAGCCAC
  CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCCCCACCACCAGAAGAGAAGGATGAAGAGTTCCCTGA
  GCAGAAAGCTGGTGAGGTGATTAACCAGCCAATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG
  AAATGGTCTAGCAAGGGCAATGACATCATTGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCAGAGATGT
  CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC
  CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT
  CTCTTACAGGTATGCGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTATCCACAGTGAAGGCCA
  CTATGCTGGGCCGGACCAACATCAGTGATGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC
  CCAGAACCTCATGCAGTCTGTGAAGGAGACTGTGCGAGAGGCTGAAGCTGCTTCAATCAAAATCCGAACA
  GATGCTGGCTTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_009502
• Insert Size: 3201 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009502.4, NP_033528.3
• RefSeq Size: 5229 bp
• RefSeq ORF: 3201 bp
• Locus ID: 22330
• UniProt ID: Q64727
• Cytogenetics: 14 11.53 cM
• Gene Summary: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).[UniProtKB/Swiss-Prot Function]