Usp25 (NM_013918) Mouse Untagged Clone

CAT#: MC223385

Usp25 (untagged) - Mouse ubiquitin specific peptidase 25 (Usp25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223385 representing NM_013918
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGTGGAGCAGAATGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACATTTTTGAATCAGC
  TGAGAGAAATTACTGGGATTAATGACGCCCAGATACTCCAACAAGCCCTGAAGGATAGTAACGGAAACTT
  GGAATTAGCAGTAGCCTTCCTCACTGCAAAGAATGCCAAGACCCCCCCACAGGAGGAGACAGGCTATTAC
  CAGACAGCGCTTCCAGGCAACGACAGGTACATCAGCGTGGGAAGCCAGGCAGATGCAAATGTAATTGATC
  TCACTGGAGATGACAAAGATGATCTTCAGAGAGCGATTGCCTTGAGTTTGGCGGAGTCAAACAGGGCATT
  CAGGGAGACGGGCATAACCGATGAAGAGCAGGCCATCAGTAGAGTTCTTGAAGCCAGCATAGCAGAAAAT
  AAAGCATGCTTGAAGAGAACACCTATAGAAGTTTGGAGGGATTCTCGAAACCCCTATGACAGAAAAAGAC
  AAGAGAAAGCTCCAGTCGGTCTGAAGAATGTCGGCAACACCTGCTGGTTTAGTGCAGTTATTCAGTCATT
  ATTCAATCTTTTGGAGTTTAGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAAGATTTACCC
  CGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGGGAACTGAGGTATCTGTTTGCACTTCTTGTTG
  GTACCAAGAGGAAGTATGTTGATCCGTCCAGAGCAGTTGAGATTCTGAAGGATGCCTTCAAATCCAATGA
  CTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAGTTATTGGACTGGTTGGAAGATGCCTTCCAAATG
  AAAGCTGAAGAGGAAACGGATGAAGAGAAGCCAAAGAACCCTATGGTAGAGCTGTTCTATGGAAGATTCC
  TAGCCATGGGAGTGCTTGAAGGAAAAAAATTTGAGAACACTGAAATGTTCGGTCAGTATCCACTTCAGGT
  GAACGGGTTCAAAGACCTGCATGAGTGCCTGGAGGCCGCCATGATCGAAGGGGAGATCGAATCCTTACAC
  TCCGATAACTCAGGAAAGTCAGGCCAGGAGCATTGGTTCACTGAATTGCCGCCGGTATTAACATTTGAAC
  TGTCAAGATTTGAATTTAACCAGGCATTGGGAAGACCAGAAAAAATTCACAATAAACTAGAATTTCCCCA
  AGTTTTATATCTGGACAGGTATATGCACAGAAATAGAGAAATAACAAGAATTAAACGTGAAGAGATCAAG
  AGACTTAAAGATTACCTCACAGTATTACAACAAAGATTAGAGAGATATTTAAGCTATGGTTCCGGTCCCA
  AAAGATTCCCATTGGTAGATGTTCTACAGTATGCATTGGAGTTTGCCTCCAGTAAACCTGTGTGCACTTC
  CCCCGTTGATGACATCGATGCTAGTTCCTCAGCAAGTGGCCCCCTACCATCACAGTCCTTACCAAGCACA
  ACAGAACAGCAGGGACCCTGTGCTTCAGATCTGCCCGGCTCCTCCTCACCCGCATCAGGTGCCGCCCTGC
  CCTTGAGGTCAGTGATTCACAAACCCTTCACTCAGTCTCGGATACCTCCAGATTTGCCCATGCATCCGGC
  ACCAAGGCACATCACAGAGGAAGAACTGTGCGTGCTGGAGAGCTGTTTACATCGCTGGAGGACGGAAATA
  GAAAATGACACGCGAGATCTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAACTCATGTACTCGG
  ACAAATCCATGATCCAAGTCCCTTACCGCCTCCATGCCGTCCTAGTTCATGAGGGCCAGGCTAATGCCGG
  CCACTACTGGGCGTACATCTTTGACCACCGGGAGAGCCGGTGGATGAAGTATAATGATATTGCTGTGACC
  AAGTCTTCATGGGAAGAGCTAGTGAGGGACTCTTTCGGTGGTTATCGAAATGCCAGTGCATACTGCTTAA
  TGTACATAGACGACAAGGCACAGTTCCTAATACAAGAGGAGTTTAATAAAGAGACTGGGCAGGCCCTTGT
  TGGAATGGAAACCTTACCTCCAGACTTGAGAGACTTTGTTGAGGAGGACAACCAGCGCTTTGAGAAGGAA
  CTGGAAGAATGGGACACGCAGCTTGCCCAACGATCACTGCAAGAAAAGCTGCTAGCTGCGCCGAAACTGC
  GGGAAGCAGAGGCTTCGGCCACCACAGCACAAGCAGGAGGCGCAGACTATCTAGAGCAGCCATCAAGAAG
  TGACTTGTCAAAGCACTGGAAAGAAGAAACGCTCCGAGTGATTGCCAAGGCGTCACATGATCTTGAAGAT
  AAAGGGCCAGAGACAGTTTTGCAGTCGGCAATTAAATTAGAGTATTCTAGGCTGGTTAAGTTGGCCCAAG
  AAGATACTCCACCAGAAACAGATTATCGTTTACATCATGTCCTGGTCTACTTTATCCAGAACCAGGCACC
  AAAGAAAATCATTGAGAAAACATTACTCGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGT
  CACAACATAATGAAAGTTGCCCAAGCCAAACTGGAAATGATCAAACCTGAAGAAGTGAACTTGGAAGAAT
  ATGAGGAGTGGCATGCAGATTATAAGAAATTCCGAGAAACAACTATGTATCTCATAACTGGACTAGAAAA
  CTTTCAGAGAGAAAGTTATATAGACTCCTTGCTGTTCCTTCTCTGTGCTTATCAGAATAACAAAGAGCTC
  TTGTCTAAGGGTCCGTACAGAGGCCACGATGGGGAGCTGATATCACATTACAGAAGAGAGTGCTTACTGA
  AATTAAACGAGCAAGCGGCCGAGCTGTTTGAGTCTGGAGAGGACGGAGACGTGAACAATGGCTTAATTAT
  TATGAATGAGTTTATCGTTCCCTTTCTGCCCTTGTTGCTGGTGGATGACATGGAAGAGAAAGATATCCTT
  GCTGTGGAAGACATGAGGAATCGCTGGTGTTCATACCTGGGGCAAGAAATGGAAGCAAACCTCCAAGAAA
  AGCTCACGGATTTTCTGCCAAAACTGCTTGATTGTTCTACAGAGATTAAAGGTTTCCACGAGCCACCAAA
  GTTACCTTCCTACTCTGCGCACGAACTCTGTGAGCGATTCGCCCGGATCATGTTGTCCCTCAGTCGTACG
  CCTGCTGATGGAAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013918
• Insert Size: 3168 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013918.2, NP_038946.2
• RefSeq Size: 4432 bp
• RefSeq ORF: 3168 bp
• Locus ID: 30940
• UniProt ID: P57080
• Cytogenetics: 16 C3.1
• Gene Summary: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]