Mfhas1 (NM_001081279) Mouse Untagged Clone

CAT#: MC223367

Mfhas1 (untagged) - Mouse malignant fibrous histiocytoma amplified sequence 1 (Mfhas1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mfhas1
• Synonyms: 2310066G09Rik; D8Ertd91e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223367 representing NM_001081279
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCCAGGACAGTGGCAACCTGAAGACGGTGAGGCTGTGGCGGGACGCCGCCCTGAGAGCCAGGA
  AGCTGCGGAGCAACCTGCGCCAGCTCACGCTCAGCTGCCCAGGGGCCGGAGGCGACCCACTGGAGTCCCC
  CGACGCCCCCCAGCTGGTGCTGCCGGCCAACATCGGGGACATTGAGGTGCTGAACCTGGGGAACAACGGC
  CTCGAGGATGTGCCTGAAGGTCTGGGGTCAGCTCTGGGCAGCCTTCGCGTCTTGGTCTTGCGCAGGAACC
  GTTTTGCCCGGCTGCCCCCTGCGGTGGCGGAGCTAGGCCACCACCTTACCGAACTGGACGTCAGCCACAA
  CCGGTTGACCATCCTGGGAGCCGAGGTGGTGAGCGCCCTGAGGGAGCTGCGCAAACTCAACCTGAGCCAC
  AACCAGTTGCCAGCCCTGCCTGCCCAGCTGGGTGCCCTCGCCCACCTGGAGGAGCTGGATGTCAGCTTCA
  ACCGGCTGGCGCATCTGCCAGATTCCTTCTCCTGCCTCAACCACCTTCGGACCCTTGACGTGGACCACAA
  CCAGCTCACCGCCTTTCCCCAGCAGCTGCTGCAGCTGGCTGCCCTGGAGGAGCTCGACGTCTCCAGCAAC
  CGGCTGCGAGGCCTACCTGAGGATATCAGTGCCCTGCGTGCCCTCAAGATCCTCTGGCTTAGCGGGGCCG
  AGCTTGGCACGCTGCCCAGAGGCTTCTGCGAGCTGGCCAGTTTGGAGAGCCTCATGTTAGATAACAACGG
  GCTCCAGGCTCTGCCCGACGAGTTCAGCCGTCTGCAGAGGCTCAAAATGCTCAACCTCTCTTCCAACCTC
  TTCGAGGAGTTCCCTGCTGCGCTGCTGCCCCTGGCTGGTCTGGAGGAGCTCTATCTTAGTCGCAATCAGC
  TCACCTCAGTGCCCTCTCTTATCGCCGGGCTGGGTCGGCTTCTCACCTTGTGGCTGGATAATAACCGCAT
  CCGGTACCTGCCAGACTCCATTGTGGAGCTGACTGGCCTGGAGGAGCTGGTGCTCCAGGGCAACCAGATC
  GCTGTGCTGCCGGACAACTTTGGCCAGCTCTCCCGGGTAGGCTTGTGGAAAATCAAGGACAACCCACTGA
  TTCAGCCCCCTTACGAAGTCTGCATGAAGGGGATCCCTTACATCGCAGCCTACCAGAAGGAACTGGCGCA
  TTCGCAGCCAGCCGTGCAGCCCCGGCTCAAGCTGCTCCTGATGGGCCACAAGGCTGCAGGAAAGACCCTG
  CTTCGACATTGCCTCACCGAGGACAAAGTGGAGGGAGGGCAAGGTGGAGGGGACAAGGAGAAGAGCTACC
  TACCTTTCCCTCCCCTTGGTAGTAAGGGCATCGAGGTGACCAGCTGGACAGCGGATGCTTCCCGGGGCTT
  GCGGTTCATTGTGTATGACTTAGCCGGTGATGAAAGCTATGAGGTGATACAGCCCTTCTTCCTCTCCCCT
  GGAGCCCTGTATGTGCTCGTGGTCAACTTGGCCACCTATGAACCTCGCTGCTTCCCTACCACCGTGGGCT
  CCTTCTTGCATCGGGTGGGGGCCCGGGTGCCTCACGCTGTGGTGTGTATTGTAGGCACGCATGCGGACCT
  GTGTGGGGAGCGGGAGCTTGAGGAGAAATGTCTGGACATTCACCGACAGATTGCCCTGCAGGAGAAGAAC
  GATGCAGAGGGCCTTAGCCATCTAGCCAAGGTGGTGGATGAGGCCCTGGCTCGGGACTTCGAGCTGCGCT
  CTGCCAGCCCTCACGCAGCCTACTACGGTGTTTCAGACAAGAACCTTCGGAGGCGCAAGGCCCATTTTCA
  ATACTTGCTTAACCACCGGTTGCAGATCCTCTCTCCCGTGTTGCCCGTAAGCTGCCGGGACCCCCTCCAA
  TTGCAACGCCTTCGAGACAAGCTGCTCTCTGTAGCTGAGCACCGAGAAATCTTTCCCAACTTACACCGAG
  TCCTGCCTCGATCCTGGCAGGTACTGGAGGAACTGCACTTCCAGCCACCTCAGGCACAACGACTGTGGCT
  GAGCTGGTGGGACTCTGCCCGCCTGGGCCTGCAGGCAGGTCTGACTGAGGACCGGCTCCAGAGTGCTCTT
  TCCTACCTGCACGAGAGCGGCAAACTGCTCTATTTTGAGGATAGCCCAGCCCTCAAGGAGCATGTCTTCC
  ACAACCTCACCCGCCTCATTGACATCCTCAATGTCTTTTTCCAGAGAGATGCTTCATTGCTGCTGCATAA
  ACTGCTCTTAGGGACCAATGGAGAGGGGGAGGGGGAGGGGGAAAGTTTCCCCACTATAGCAGTGCCCAGC
  CCGGGTCAGGACCCACTCCGGGCCACCCAGCTTCATCATTATGTGGAAGGCTTTTTGCTGCATGGGCTTC
  TGCCAGCCCACATCATTCGGTTGCTGCTTAAGCCTCATGTCCAGGCCCAACAGGACTTGCAGCTCCTGCT
  GGAGCTGTTGGAGAAGATGGGACTCTGTTACTGCCTCAATAAACCCAAAGGCAAGCCTTTGAATGGCTCC
  GCGGCATGGTACAAGTTCCCATGCTACGTGCAGAATGAGGTGCCCCATGCAGAGGCCTGGATTAATGGCA
  CCAACCTAGCCGGGCAGTCTTTTGTGGCTGAACAGTTACAGATCGAATATAGTTTTCCCTTCACCTTTCC
  ACCTGGATTGTTCGCACGTTACAGTGTCCAGATCAACAGTCACGTGGTGCACAGGTCGGATGGGAAATTT
  CAGATCTTTGCATATAGAGGGAAAGTTCCCGTGGTGGTGAGTTACAGACCTGCCAAGGGGGTTCTGCAGC
  CCGACACCCTGTCCATTGCCAGCCATGCGTCACTACCAAATATATGGACCGCATGGCAAGCCATCACCCC
  TTTGGTAGAGGAACTGAATGTCCTACTTCAGGAATGGCCTGGACTGCACTACACTGTGCACATCCTCTGT
  TCTAAGTGCCTTAAGAGAGGGTCGCCAAATCCACACGCTTTCCCAGGGGAGTTGCTGAGCCAGCCCAGGC
  CAGAAGGGGTGGCAGAGATCATCTGCCCCAAGAATGGCAGCGAGCGAGTGAATGTGGCTTTGGTTTACCC
  ACCTACACCGACTGTGATCAGCCCTTGTTCCAAGAAGAACGTTGGTGAGAAGCACAGAAACCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081279
• Insert Size: 3147 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081279.1, NP_001074748.1
• RefSeq Size: 6408 bp
• RefSeq ORF: 3147 bp
• Locus ID: 52065
• UniProt ID: Q3V1N1
• Cytogenetics: 8 21.16 cM
• Gene Summary: Probable GTP-binding protein (By similarity). Functions in innate immunity and more specifically the inflammatory response as a regulator of the Toll-like receptor TLR2 and TLR4 signaling pathways (PubMed:20616063, PubMed:26599367). Negatively regulates the part of the TLR4 signaling pathway that leads to the activation of the transcription factor AP-1. By retaining the phosphatase complex PP2A into the cytoplasm, prevents the dephosphorylation of the AP-1 subunit JUN which is required for proper activation of the transcription factor (By similarity). Both inhibits and activates the TLR2-dependent signaling pathway (PubMed:26599367). Positively regulates the TLR2 signaling pathway to activate specifically the downstream p38 and JNK MAP kinases and promote the polarization of macrophages toward the pro-inflammatory M1 phenotype. It may also play a role in the regulation of inflammation induced by high glucose through the PKB/AKT signaling pathway. Also involved in erythrocyte differentiation through activation of the ERK1/ERK2 signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]