Ptk2 (NM_007982) Mouse Untagged Clone

CAT#: MC223361

Ptk2 (untagged) - Mouse PTK2 protein tyrosine kinase 2 (Ptk2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptk2
• Synonyms: Fadk; FADK 1; FAK; FRNK; mKIAA4203; p125FAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223361 representing NM_007982
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGCTTATCTTGACCCAAACTTGAATCACACACCAAGTTCGAGTACTAAGACTCACCTGGGTA
  CTGGCATGGAACGGTCCCCTGGTGCAATGGAACGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAGTAG
  TGAGCCAACCACCTGGGCCAGTATTATCAGGCATGGAGATGCTACTGATGTCAGGGGCATCATTCAGAAG
  ATAGTGGACAGTCACAAAGTAAAGCACGTGGCCTGCTATGGATTTCGCCTCAGTCACCTGCGATCGGAGG
  AGGTGCACTGGCTGCACGTGGACATGGGTGTCTCCAGTGTGAGGGAGAAATATGAACTCGCCCACCCACC
  AGAGGAGTGGAAATATGAGTTGAGAATTCGTTACTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGAT
  AAGCCAACATTGAATTTCTTCTATCAACAGGTGAAGAGTGACTACATGCAAGAAATAGCTGATCAAGTAG
  ACCAAGAAATAGCTTTGAAGTTGGGTTGTTTGGAAATTAGGCGATCCTATTGGGAGATGAGGGGTAATGC
  CCTAGAGAAGAAGTCCAACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAGT
  TTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAGCTGATCCAGCAAACATTCAGGCAGTTTGCCAACC
  TTAATAGAGAAGAAAGCATCCTGAAATTCTTTGAGATTCTTTCTCCTGTGTACAGATTTGACAAAGAGTG
  CTTCAAGTGTGCCCTTGGGTCAAGTTGGATCATTTCTGTGGAATTGGCAATCGGCCCAGAAGAAGGGATC
  AGTTACCTGACAGACAAAGGCTGCAATCCCACACACCTAGCAGACTTTAACCAAGTGCAGACCATCCAGT
  ACTCAAACAGTGAAGACAAAGACAGGAAAGGAATGCTACAACTCAAAATCGCTGGTGCTCCTGAGCCTCT
  GACCGTGACGGCACCATCCCTAACCATTGCCGAGAACATGGCCGACCTGATTGATGGATACTGCCGGCTG
  GTGAATGGAGCCACACAATCTTTCATCATCAGACCCCAGAAAGAAGGTGAACGGGCGTTGCCATCAATAC
  CAAAGTTGGCCAACAGTGAAAAGCAAGGCATGCGGACACATGCAGTCTCTGTGTCAGAGACAGATGACTA
  TGCAGAGATCATCGATGAGGAAGACACATACACCATGCCCTCGACCAGGGATTATGAGATTCAGAGAGAA
  AGAATAGAACTTGGACGCTGTATTGGAGAAGGTCAGTTTGGAGATGTACATCAAGGCGTGTACCTGAGCC
  CAGAGAATCCAGCTTTGGCTGTTGCAATCAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAA
  GTTCCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCACATCGTGAAGCTGATTGGAGTCATT
  ACAGAGAACCCTGTCTGGATAATCATGGAGTTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAA
  GAAAATACAGCTTGGACCTGGCATCTTTGATATTATATGCCTATCAGCTTAGTACAGCACTCGCGTATCT
  GGAGAGCAAAAGATTTGTTCACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCTTCAAATGATTGTGTA
  AAATTGGGAGACTTTGGATTATCTCGATATATGGAAGACAGTACTTACTATAAAGCTTCCAAAGGAAAAT
  TACCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTGTGGAT
  GTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGCGTGAAGCCTTTTCAAGGAGTGAAGAACAATGAT
  GTGATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCCACCCTCTACAGCC
  TTATGACGAAATGTTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACTAAAAGCTCAGCTCAG
  CACAATCCTGGAGGAGGAGAAGGTGCAGCAAGAAGAACGGATGAGGATGGAATCCAGAAGACAGGCTACC
  GTGTCCTGGGACTCTGGAGGGTCTGATGAAGCACCACCCAAGCCCAGCAGACCTGGTTATCCTAGCCCGA
  GATCCAGTGAAGGATTTTATCCCAGCCCACAGCACATGGTCCAGACCAATCACTACCAGGTCTCTGGCTA
  CCCGGGTTCCCATGGAATCCCAGCCATGGCTGGCAGCATTTACCAAGGTCAGGCATCTCTTCTGGACCAG
  ACAGAATTGTGGAATCACAGACCTCAGGAGATGTCCATGTGGCAGCCCAGTGTGGAGGATTCGGCAGCTT
  TGGACCTTCGAGGGATGGGACAGGTGCTTCCCCCTCACCTGATGGAAGAGCGGCTAATCCGACAGCAGCA
  GGAAATGGAAGAAGACCAGCGCTGGCTGGAGAAGGAAGAGCGATTCCTGAAACCTGATGTGAGGCTCTCT
  CGAGGCAGCATCGACAGGGAAGACGGGAGTTTTCAGGGTCCGACTGGAAACCAACACATCTATCAGCCTG
  TGGGGAAGCCAGATCCTGCAGCTCCACCAAAGAAACCTCCTCGCCCTGGAGCACCTGGCCACCTAAGCAA
  CCTGTCCAGCATCAGCAGCCCTGCAGACAGCTACAATGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGC
  CCCCCTCCCACTGCCAACCTTGACCGGTCCAATGACAAGGTATATGAAAATGTGACAGGCCTAGTGAAGG
  CTGTCATCGAGATGTCCAGCAAAATCCAGCCAGCTCCTCCAGAAGAGTACGTCCCTATGGTGAAGGAAGT
  TGGCCTGGCCCTGCGGACCTTACTAGCCACGGTGGATGAGACCATTCCTGCTCTTCCAGCCAGCACTCAT
  CGAGAGATCGAGATGGCACAGAAGCTGCTGAACTCCGACTTAGGCGAGCTCATCAGCAAGATGAAGCTGG
  CGCAGCAGTACGTCATGACCAGCCTGCAGCAGGAGTATAAGAAGCAGATGCTGACAGCTGCTCACGCCCT
  GGCTGTGGATGCCAAGAACCTACTTGATGTTATTGATCAAGCAAGACTGAAAATGCTAGGGCAGACACGG
  CCACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007982
• Insert Size: 3159 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007982.2, NP_032008.2
• RefSeq Size: 4414 bp
• RefSeq ORF: 3159 bp
• Locus ID: 14083
• UniProt ID: P34152
• Cytogenetics: 15 33.94 cM
• Gene Summary: Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 9 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1.