Gucy2c (NM_145067) Mouse Untagged Clone

CAT#: MC223348

Gucy2c (untagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gucy2c
• Synonyms: AI893437; GC-C; Gcc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223348 representing NM_145067
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCACTGCTGGGCTTGGCTGTGCGGTTACTGCTCTTCCAGCCCGCGCTGATGGTGTTCTGGGCCT
  CTCAGGTGAGGCAGAACTGCCGCAATGGCAGCTACGAGATCAGCGTCCTGATGATGGACAACTCAGCCTA
  CAAAGAACCTATGCAAAACCTGAGGGAGGCTGTGGAGGAAGGACTGGACATAGTGCGAAAGCGCCTGCGT
  GAAGCCGACCTAAATGTGACTGTGAACGCGACTTTCATCTACTCCGACGGTCTGATTCATAAGTCAGGTG
  ACTGCCGGAGCAGCACCTGTGAAGGCCTTGACCTACTCAGGGAGATTACAAGAGATCATAAGATGGGCTG
  CGCCCTCATGGGGCCCTCGTGCACGTATTCCACCTTCCAGATGTACCTCGACACAGAGTTGAACTATCCC
  ATGATTTCCGCTGGAAGTTATGGATTGTCCTGTGACTATAAGGAAACCCTAACCAGGATCCTGCCTCCAG
  CCAGGAAGCTGATGTACTTCTTGGTCGATTTCTGGAAAGTCAACAATGCATCTTTCAAACCCTTTTCCTG
  GAACTCTTCGTATGTTTACAAGAATGGATCGGAACCTGAAGATTGTTTCTGGTACCTCAATGCTCTGGAG
  GCTGGGGTGTCCTATTTTTCTGAGGTGCTCAACTTCAAGGATGTACTGAGACGCAGCGAACAGTTCCAGG
  AAATCTTAACAGGCCATAACAGAAAGAGCAATGTGATTGTTATGTGTGGCACGCCAGAAAGCTTCTATGA
  TGTGAAAGGTGACCTCCAAGTGGCTGAAGATACTGTTGTCATCCTGGTAGATCTGTTCAGTAACCATTAC
  TTTGAGGAGAACACCACAGCTCCTGAGTATATGGACAATGTCCTCGTCCTGACGCTGCCGTCTGAACAGT
  CCACCTCAAACACCTCTGTCGCCGAGAGGTTTTCATCGGGGAGAAGTGACTTTTCTCTCGCTTACTTGGA
  GGGAACCTTGCTATTTGGACACATGCTGCAGACGTTTCTTGAAAATGGAGAAAATGTCACGGGTCCCAAG
  TTTGCTCGTGCATTCAGGAATCTCACTTTTCAAGGCTTTGCAGGACCTGTGACTCTGGATGACAGTGGGG
  ACATTGACAACATTATGTCCCTTCTGTATGTGTCTCTGGATACCAGGAAATACAAGGTTCTTATGAAGTA
  TGACACCCACAAAAACAAAACTATTCCGGTGGCTGAGAACCCCAACTTCATCTGGAAGAACCACAAGCTC
  CCCAATGACGTTCCTGGGCTGGGCCCTCAAATCCTGATGATTGCCGTCTTCACGCTCACGGGGATCCTGG
  TAGTTCTGCTGCTGATTGCCCTCCTCGTGCTGAGAAAATACAGAAGAGATCATGCACTTCGACAGAAGAA
  ATGGTCCCACATTCCTTCTGAAAACATCTTTCCTCTGGAGACCAACGAGACCAACCACATCAGCCTGAAG
  ATTGACGATGACAGGAGACGAGACACAATCCAGAGAGTGCGACAGTGCAAATACGACAAGAAGTTGCTGC
  AGTCTGACTACTACAACCTGACTAAGTTCTACGGCACCGTGAAGCTGGACACCAGGATCTTTGGGGTGGT
  TGAGTACTGCGAGAGGGGATCCCTCCGGGAAGTGTTAAACGACACAATTTCCTACCCTGACGGCACGTTC
  ATGGATTGGGAGTTTAAGATCTCTGTCTTAAATGACATCGCTAAGGGGATGTCCTACCTGCACTCCAGTA
  AGATTGAAGTCCACGGGCGTCTCAAATCCACCAACTGCGTGGTGGACAGCCGCATGGTGGTGAAGATCAC
  CGACTTTGGGTGCAATTCCATCCTGCCTCCAAAAAAAGACCTGTGGACGGCCCCGGAGCACCTGCGCCAG
  GCCACCATCTCTCAGAAAGGAGACGTGTACAGCTTCGCCATCATTGCCCAGGAGATCATCCTCCGTAAGG
  AGACTTTTTACACGCTGAGCTGTCGGGATCACAATGAGAAGATTTTCAGAGTGGAAAATTCATACGGGAA
  ACCTTTCCGCCCAGACCTCTTCCTGGAGACTGCAGATGAGAAGGAGCTGGAGGTCTATCTACTTGTCAAA
  AGCTGTTGGGAGGAGGATCCAGAAAAGAGGCCAGATTTCAAGAAAATCGAGAGCACACTGGCCAAGATAT
  TTGGCCTTTTCCATGACCAGAAAAACGAGTCTTACATGGACACCTTGATCCGACGTCTCCAGCTGTACTC
  TCGAAACCTGGAACATCTGGTGGAGGAAAGGACTCAGCTGTACAAGGCGGAGAGGGACAGGGCTGACCAC
  CTTAACTTCATGCTCCTCCCACGGCTGGTGGTAAAGTCACTGAAGGAGAAAGGCATCGTGGAGCCAGAGC
  TGTACGAAGAAGTCACAATCTACTTCAGTGACATTGTGGGCTTCACCACCATCTGCAAGTATAGCACGCC
  CATGGAGGTGGTGGACATGCTCAACGACATCTACAAGAGCTTTGACCAGATTGTGGACCACCATGACGTC
  TACAAGGTAGAAACCATCGGTGACGCCTACGTGGTGGCCAGCGGTCTGCCTATGAGAAACGGCAACCGAC
  ACGCGGTAGACATTTCCAAGATGGCCTTGGACATCCTCAGCTTCATAGGGACCTTTGAGTTGGAGCATCT
  CCCTGGCCTCCCCGTGTGGATCCGCATTGGAGTTCATTCTGGGCCCTGCGCTGCTGGTGTTGTGGGGATC
  AAGATGCCTCGCTATTGCCTGTTTGGAGACACTGTCAACACTGCCTCCAGGATGGAATCCACCGGCCTCC
  CCTTGAGGATTCACATGAGCAGCTCCACCATAACCATCCTGAAGAGAACGGATTGCCAGTTCCTGTATGA
  AGTGAGGGGAGAAACCTACTTAAAGGGAAGAGGGACAGAGACCACATACTGGCTGACTGGGATGAAGGAC
  CAAGAATACAACCTGCCATCCCCACCGACAGTGGAGAACCAACAGCGTCTGCAGACTGAGTTCTCAGACA
  TGATCGTTAGCGCCTTACAGAAAAGACAGGCCTCGGGCAAGAAGAGCCGGAGGCCCACTCGGGTGGCCAG
  CTACAAGAAAGGCTTTCTGGAATACATGCAGCTGAACAATTCAGACCACGATAGCACCTATTTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145067
• Insert Size: 3147 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145067.3, NP_659504.2
• RefSeq Size: 3879 bp
• RefSeq ORF: 3147 bp
• Locus ID: 14917
• UniProt ID: Q3UWA6
• Cytogenetics: 6 66.67 cM
• Gene Summary: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.