Atp9a (NM_015731) Mouse Untagged Clone

CAT#: MC223345

Atp9a (untagged) - Mouse ATPase, class II, type 9A (Atp9a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp9a
• Synonyms: IIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223345 representing NM_015731
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGACAGCATCCCGCTGCAGCCCGTGCGCCACAAGAAGCGGGTGGACAGTAGGCCGCGCGCGGGGT
  GCTGTGAGTGGCTGAGATGTTGCGGTGGAGGGGAGCCCAGGCCCCGTACTGTCTGGTTGGGACACCCCGA
  GAAGAGGGACCAGCGGTACCCTCGAAATGTCATCAACAACCAGAAGTACAATTTCTTCACATTTCTTCCT
  GGGGTGTTGTTCAGCCAGTTCAGATACTTCTTCAACTTCTACTTCCTGCTTCTCGCCTGCTCGCAGTTCG
  TCCCAGAGATGAGGCTTGGCGCCCTGTACACCTACTGGGTTCCTCTGGGCTTCGTGCTGGCTGTCACCAT
  CATCCGTGAGGCAGTAGAGGAGATCCGATGTTATGTGCGTGACAAGGAGATGAACTCCCAGGTCTACAGC
  CGGCTCACGTCACGAGGGACCGTGAAGGTGAAGAGTTCAAACATCCAGGTGGGAGACCTCATCCTTGTGG
  AAAAGAACCAGCGGGTCCCTGCTGACATGATCTTCCTGAGGACGTCAGAGAAAAACGGCTCTTGCTTCTT
  GCGCACGGATCAGCTGGATGGAGAGACAGACTGGAAGCTTCGGCTCCCGGTGGCCTGCACACAGAGGCTT
  CCCACGGCTGCTGACCTCCTGCAGATTCGGTCCTATGTGTACGCTGAAGAGCCCAACATCGACATTCACA
  ACTTCCTGGGGACTTTCACCAGGGAAGACAGTGACCCTCCGATCAGTGAGAGTCTGAGCATTGAGAACAC
  GCTGTGGGCCGGCACCGTCATAGCATCAGGCACTGTTGTAGGCGTTGTTCTCTACACTGGCAGAGAACTG
  CGGAGTGTCATGAATACTTCCGACCCCAGAAGTAAGATTGGCCTGTTCGACCTGGAGGTGAACTGCCTCA
  CCAAGATCCTGTTTGGTGCGCTGGTGGTGGTGTCGCTGGTCATGGTGGCCCTGCAGCACTTTGCCGGCCG
  CTGGTACCTGCAGATCATCCGCTTCCTGCTCCTGTTTTCCAACATCATTCCTATCAGCTTGCGTGTGAAC
  TTGGACATGGGCAAGATCGTGTACAGCTGGGTGATCCGCAGGGATTCCAAAATCCCCGGGACCGTGGTTC
  GTTCCAGCACAATTCCTGAGCAGCTGGGCAGGATTTCGTACTTGCTCACAGACAAGACAGGAACCCTGAC
  CCAGAATGAGATGGTGTTCAAGCGGCTGCACCTGGGTACGGTGGCCTACGGCCTGGACTCCATGGACGAA
  GTGCAGAGTCACATCTTCAGCATTTACACCCAGCAATCCCAGGATCCACCTGCTCAGAAGGGCCCCACGG
  TCACCACCAAGGTTCGGAGGACCATGAGCAGCCGTGTCCACGAGGCTGTGAAGGCCATTGCACTCTGCCA
  CAACGTGACACCCGTGTACGAGTCCAATGGTGTGACGGACCAGGCTGAGGCTGAGAAGCAGTTTGAGGAC
  TCCTGCCGAGTGTACCAGGCATCCAGCCCGGATGAGGTGGCTCTGGTCCAGTGGACAGAAAGTGTGGGAC
  TGACGCTGGTGGGTCGAGACCAGTCCTCCATGCAGCTGAGGACCCCTGGTGACCAGGTCCTGAATCTCAC
  TATCCTTCAGGTCTTCCCGTTCACCTATGAGAGCAAGCGGATGGGCATCATCGTGCGGGATGAGTCCACG
  GGGGAAATCACGTTCTACATGAAGGGAGCAGACGTCGTCATGGCTGGCATTGTCCAGTACAACGACTGGC
  TGGAGGAGGAGTGTGGCAACATGGCCCGGGAGGGACTACGTGTGCTGGTGGTAGCCAAGAAGTCCCTCAC
  AGAGGAGCAGTACCAAGACTTTGAAGCCCGCTACGTCCAGGCTAAGCTGAGTGTGCATGACCGCTCGCTG
  AAGGTGGCCACGGTGATCGAGAGCCTGGAGATGGAGATGGAGCTGCTGTGCCTGACTGGTGTGGAGGACC
  AGCTGCAGGCAGATGTCAGGCCCACGCTGGAGACGCTGCGCAACGCTGGCATCAAGGTTTGGATGCTAAC
  AGGGGACAAGCTGGAGACAGCCACGTGCACAGCCAAGAACGCACATCTGGTGACCAGAAACCAAGATATC
  CATGTTTTCCGACTGGTGACCAACCGCGGGGAGGCCCACCTGGAGCTGAATGCCTTCCGTAGGAAGCATG
  ACTGTGCCCTGGTCATCTCTGGAGACTCCCTGGAGGTTTGCCTCAAATACTATGAGTACGAGTTCATGGA
  ACTGGCCTGCCAGTGCCCGGCTGTGGTGTGCTGCCGCTGTGCCCCAACCCAGAAGGCCCAGATTGTTCGG
  CTGCTCCAGGAACGCACCGGGAAGCTCACCTGTGCAGTAGGGGACGGAGGCAATGACGTCAGCATGATCC
  AGGAATCCGACTGCGGCGTGGGCGTGGAGGGCAAGGAAGGGAAGCAGGCCTCGCTGGCAGCGGACTTCTC
  CATCACCCAGTTCAAGCATCTCGGCCGCTTGCTCATGGTGCACGGTCGGAACAGCTACAAGCGCTCGGCG
  GCCCTCAGTCAGTTTGTGATCCACAGGAGCCTCTGCATCAGCACCATGCAGGCTGTCTTCTCGTCTGTGT
  TCTACTTTGCATCCGTTCCTCTCTACCAAGGCTTCCTGATCATTGGGTATTCTACCATCTACACGATGTT
  TCCCGTGTTCTCCCTGGTTTTGGACAAAGACGTGAAGTCGGAAGTCGCCATGTTGTATCCTGAGCTCTAC
  AAGGACCTGCTTAAGGGGCGGCCACTGTCCTACAAGACGTTCTTAATTTGGGTGTTAATCAGCATCTATC
  AAGGGAGCACCATCATGTACGGGGCGCTGCTGCTGTTCGAGTCGGAGTTTGTACACATCGTGGCAATCTC
  CTTCACATCCCTCATCCTCACTGAGCTACTGATGGTGGCGCTCACCATCCAGACGTGGCACTGGCTCATG
  ACAGTGGCCGAGCTACTCAGCCTGGCCTGCTACATTGCCTCCCTGGTGTTCCTCCATGAGTTCATCGATG
  TCTACTTCATTGCCACCCTGTCATTCCTCTGGAAGGTGTCCGTCATCACCTTGGTCAGCTGTCTCCCCCT
  CTATGTCCTCAAGTACCTGCGGAGACGGTTCTCCCCACCCAGCTACTCGAAGCTCACTTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015731
• Insert Size: 3144 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015731.3, NP_056546.2
• RefSeq Size: 3529 bp
• RefSeq ORF: 3144 bp
• Locus ID: 11981
• UniProt ID: O70228
• Cytogenetics: 2 H3
• Gene Summary: Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling and endosome to trans-Golgi network retrograde transport. In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation. Appears to be implicated in intercellular communication by negatively regulating the release of exosomes. The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.