Ssh1 (NM_198109) Mouse Untagged Clone

CAT#: MC223338

Ssh1 (untagged) - Mouse slingshot homolog 1 (Drosophila) (Ssh1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ssh1
• Synonyms: AW551225; Gm1394; Gm1395; SSH-1; SSH-1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223338 representing NM_198109
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGGTGACCCTTCAGCGCTCTCCCACGCCCAGCGCCGCGTCTTCCTCCGCAAGCAACAGCGAGT
  TGGAGGCTGGCAGCGATGAAGAACGGAAATTGAACCTCAGCTTGAGTGAGAGCTTTTTCATGGTGAAAGG
  AGCTGCCCTCTTCCTACAGCAGGGAAACAGCCCACAGGGCCAGCGGAGTCTTCAGCACCCTCACAAGCAT
  GCAGGTGATCTGCCTCAGCACCTGCAAGTGATGATCAACCTCCTGCGTTGTGAAGACAGAATCAAGCTGG
  CCGTGCGCCTAGAGAGTGTCTGGACCGACCGTGTCCGCTACATGGTCGTGGTATACACCAGCGGGCGCCA
  GGACACCGAGGAGAATATTCTGCTGGGAGTTGACTTTTCCAGTAAGGAGAGCAAAAGCTGCACGATCGGA
  ATGGTTCTTCGACTCTGGAGCGACACCAAGATTCACCTCGATGGGGACGGCGGGTTCAGTGTGAGCACAG
  CGGGCAGGATGCACATATTCAAGCCAGTGTCTGTCCAGGCCATGTGGTCTGCCCTGCAAGTGCTTCACAA
  GGCCTGCGAAGTGGCCCGGAGGCATAACTACTTCCCAGGAGGAGTGGCGCTCATCTGGGCCACCTACTAT
  GAGAGCTGCATCAGCTCGGAGCAGAGCTGCATCAATGAGTGGAATGCCATGCAGGACCTGGAGTCCACGC
  GGCCCGACTCCCCCGCGCTGTTTGTGGACAAGCCAACCGAGGGCGAAAGAACTGAGCGCCTCATTAAAGC
  CAAACTCCGGAGCATCATGATGAGCCAGGACCTTGAAAATGTGACTTCTAAGGAAATCCGAAATGAGCTG
  GAGAAGCAAATGAACTGCAACCTGAAGGAGTTCAAGGAATTCATCGATAACGAGATGCTGCTCATCCTGG
  GCCAGATGGACAAGCCCTCCCTCATCTTTGACCATCTTTATCTTGGCTCCGAGTGGAATGCATCCAATCT
  GGAGGAGCTGCAGGGCTCAGGAGTTGACTACATTTTAAATGTCACTAGAGAAATAGACAATTTTTTCCCC
  GGCTTGTTTGCGTACCATAACATCCGCGTGTACGATGAGGAGACCACAGACCTTCTTGCCCACTGGAATG
  AAGCTTATCATTTCATAAACAAAGCGAAAAGGAATCATTCCAAGTGCCTGGTCCATTGCAAGATGGGCGT
  CAGCCGATCTGCGTCCACAGTCATTGCCTACGCCATGAAGGAGTTCGGCTGGCCCCTGGAGAAAGCGTAT
  AACTACGTGAAGCAGAAACGTAGTATCACTCGGCCCAACGCAGGCTTTATGAGGCAGCTGTCTGAGTACG
  AAGGCATCCTGGATGCAAGCAAACAGCGGCATAACAAACTATGGCGCCAGCAGCCCACAGATGACACCAT
  CGCAGAGCCCAGTGAGTTCTTGCCAGAGACCTTGGACGGAGCCCTGGACGCTCAGCTGCCCTGTTTGGAT
  GATACCACCCACCCTGGGCTCCCGAGAAGTCTGGCCCCAGGAGGACCCGCTCTCCCCTGTTGTTTTCGAA
  GACTCTCGGACCCCCTCCTCCTTCCCCACCATGATGAAACGGGTGGCCTGGTCCACTTAGAGGATCTCGA
  GAAGGATGCTCTGTTAGAGGAGGAGGAGTCTCAGCCAGTGGAGGTGCACAAGCTGGTTCAGCATCCCCAG
  GAAGGTGCCAGGCTGTGTGAAAAGGACGTAAAGAGGAAACTGGAGTTTGGGAACTCCAAACCCCGCAGTG
  ACTCCTTGCCTCAGGTGGAGGAGCTGGAGAAGGACGGTAGCCCAAGAACGGGGAGGTGGAGGCGGGCCTC
  CACCCAGCTCGATAGAAGCTTGCTCGACCAGGAAAACCTAAATAACAACAACAGCAAGAGGAGCTGTCCC
  GATGACTTGGAGCGCGATGCCATGTTTGGAATTCTCAGCAAAGTGAAGCCTCCCTACACATCCTGTGCCG
  ACTGCATGTACCCCACAGCTGGTGGGACTCCCGAGGCCTACATGGAGCGACACGAAGACCCCAGCTCCTC
  TGCTATCTGCACCCAGCCAACCTTCCTCCCCCATGTCACGTCTTCCCCAATGGCCCACGCGAGCAGCAGG
  TCCCGAGCTCCGGAGAGGCCGGCCTCGGGTCCAGCCAACACCTCTCCATTCCTACTACCAGCAGGCTCCA
  GGAAGCCAGATGTCAGTGGCTCTGGAGCCGGGGCTGCCCCGGAACCACCAGCAAGCCTTCTAGAGCCTTC
  CAGAGAGACCTCAAAAGCCCTTCCAAAATCTCTCCAGTTGAAGAATCCTCACTGTGACAAGAACGCTGCC
  AATATGGAGGTGTCGGCGAAGGAAGAACCGTCGCCTAAGAAAGATCCCAAGCCGGCTAAGGACCTGAGGC
  TTCTGTTCAGTAACGAGGCGGAGAAGCCCACCACCAACAGCTACCTGATGCAGCATCAGGAGTCCATCAT
  CCAACTGCAGAAGGCAGGCCTGGTCCGAAAGCACACCAAAGAGCTGGAGAGGTTGAAGAGCCTGCCTTCA
  GACTCACCAGCTGCCTGCAGGGACAGCGCCACCTGCAGGCTGGAGGCCAGCATCCCGGAGGAGGGTAGCC
  AGGAGCCCGCACACCCAGCCCTGTGCAGCCAAGCTGGGTCGGAAGAACAGCCTGTAGGGGGAACCTTGCA
  GAAGAGCCCCACGTCTACCCTCCCCCGTTTAGATCACACCAGTAACTTCTCGAAGGACTTCCTGAAGACC
  GTGTGTTACACCCCCACCTCCTCCTCCATCAGCTCCAACCTGACCCGGAGCTCCAGCAGCGACAGCATCC
  ACAGCGTCCGAGGGAAGCCTGGGCTGGTGAAGCAGCGGGCGCAGGAGATCGAGACGCGGCTGCGCCTGGC
  AGGCCTCACCGTGTCGTCCCCGCTGAAAAGGTCCCATTCCCTTGCCAAGCTGGGAAGTCTCAACTTCTCC
  ACGGAGGACCTGTCCAGCGAGGCTGACACATCCACCATCGCTGACTCGCAGGACGCCAAGTGTGGTCTCT
  CCTCTTCCTTCTTGCCCGAACCCCAGTCTGCGCCAAGGGACCCCGCTGCAACCTCTAAATCATCAGGGAA
  ATCTGCCCCAGAACACTTGAAAAGCCCGTCGAGGGTAAACAAAAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198109
• Insert Size: 3129 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198109.4, NP_932777.2
• RefSeq Size: 3174 bp
• RefSeq ORF: 3129 bp
• Locus ID: 231637
• UniProt ID: Q76I79
• Cytogenetics: 5 F
• Gene Summary: Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.[UniProtKB/Swiss-Prot Function]