Itgae (NM_172944) Mouse Untagged Clone

CAT#: MC223314

Itgae (untagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itgae
• Synonyms: A530055J10; alpha-E1; alpha-M290; aM290; CD103
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223314 representing NM_172944
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGGCTCTTCCATACCCTTCTCTGCATGGCCAGCCTGAAGCCCCAGGGAGCCTTCAATTTGGATG
  TGGACTGGGCCTGGGTCACAGCACTCCAGCCAGGTGCACCGGCTGTGCTCAGCTCCCTTCTGCATCAAGA
  CCCCAGCAACAACCAGACCTGCCTCCTGGTAGCCAGACGCAGCTCCAACAGGAATACAGCCGCCCTGTAT
  CGATGCGCTATATCCATCAGTCCAGATGAAATTGCTTGCCAGCCAGTGGAGCACATCTGTATGCCAAAGG
  GCAGGTACCAGGGAGTGACATTGGTCGGGAACCACAATGGTGTTTTGGTGTGCATTCAGGTACAGGCCCG
  GAAGTTCCGCAGCCTCAACTCAGAGCTCACGGGTGCCTGCAGCCTACTCACCCCCAATTTGGACCTCCAG
  GCCCAAGCCTACTTCTCTGACCTCGAAGGGTTCCTGGATCCAGGAGCACATGTGGACTCTGGTGACTACT
  GTAGAAGCAAAGGAGGCAGCACAGGGGAGGAGAAGAAGTCAGCCAGGAGACGCCGGACAGTGGAGGAGGA
  AGACGAGGAGGAAGATGGCACTGAGATCGCTATTGTCCTGGATGGCTCAGGAAGCATTGAACCCTCAGAC
  TTCCAGAAGGCCAAAAATTTCATCTCTACCATGATGAGGAACTTCTATGAGAAGTGTTTTGAGTGCAACT
  TTGCCCTGGTGCAGTATGGGGCCGTGATCCAGACTGAGTTTGATCTTCAAGAGAGCCGGGACATTAACGC
  CTCCCTTGCCAAAGTTCAGAGCATCGTTCAAGTGAAGGAAGTCACTAAGACCGCCTCAGCCATGCAGCAT
  GTCCTAGACAATATCTTCATTCCAAGCCGTGGCTCCAGAAAAAAAGCATTGAAGGTCATGGTGGTACTTA
  CTGATGGTGACATATTTGGGGACCCTCTCAATCTTACGACTGTCATCAACTCCCCAAAGATGCAGGGTGT
  TGTGCGCTTTGCCATTGGGGTGGGTGATGCATTCAAGAATAACAACACTTACAGAGAACTGAAGCTGATT
  GCCTCAGACCCCAAAGAGGCCCACACTTTCAAGGTGACCAACTACTCAGCTCTGGATGGGCTGCTGAGCA
  AGCTGCAACAACACATCGTTCATATGGAAGGTACAGTTGGAGATGCCCTTCAATATCAACTCGCACAGAC
  TGGTTTCAGTGCCCAGATCCTGGACAAGGGGCAGGTGTTGCTTGGCACTGTGGGGGCCTTTAACTGGTCT
  GGGGGTGCGCTGCTCTACAGCACACAGAATGGCCGGGGCTGCTTCCTAAACCAGACAGCCAAGGAGGACT
  CCAGGACTGTACAGTACAGCTACCTAGGTTACTCCCTTGCGGTGCTACACAAGGCCCACGGCGTCTCTTA
  CGTGGCAGGGGCTCCAAGGCACAAGCTCCGTGGGGCTGTGTTTGAGCTCCGGAAGGAGGACAGAGAGGAG
  GATGCCTTTGTGCGACGGATAGAAGGAGAGCAGATGGGGTCCTACTTTGGCTCTGTGCTGTGCCCTGTGG
  ATATCGACATGGATGGGACCACAGATTTTCTACTGGTGGCTGCTCCATTTTACCACATCCGTGGAGAAGA
  AGGCAGAGTCTATGTGTACCAGGTGCCTGAGCAGGATGCTTCCTTCTCCTTGGCACACACACTGAGTGGG
  CACCCTGGGCTCACCAATAGCCGCTTTGGCTTTGCCATGGCAGCTGTGGGGGATATCAACCAAGACAAAT
  TCACAGATGTGGCCATTGGGGCCCCTCTGGAGGGTTTTGGGGCAGGTGACGGGGCCAGCTATGGCAGCGT
  GTACATCTACAACGGACACTCAGGTGGCTTGTATGACAGTCCTTCCCAGCAGATCCGAGCCTCCTCTGTG
  GCCTCGGGACTCCACTACTTCGGCATGTCTGTGTCCGGTGGGTTAGATTTCAATGGCGATGGCCTTGCCG
  ATATCACAGTTGGCTCTCGGGACTCGGCGGTTGTGCTCCGCTCACGCCCTGTGGTTGACCTGACCGTATC
  TATGACCTTTACCCCCGATGCACTGCCCATGGTCTTCATAGGCAAGATGGATGTAAAACTGTGTTTTGAA
  GTTGACTCTTCAGGCGTAGCTTCTGAGCCAGGCCTTAGAGAGATGTTCCTCAACTTTACGGTGGATGTGG
  ATGTGACCAAGCAAAGGCAGAGGCTGCAGTGTGAGGACTCGAGCGGTTGTCAGAGCTGCCTCAGGAAGTG
  GAACGGAGGGTCCTTTCTGTGTGAACACTTCTGGCTCATCTCCACAGAGGAGCTTTGTGAGGAGGACTGC
  TTCTCCAACATCACCATCAAGGTCACCTACGAGTTCCAGACGTCTGGGGGAAGGAGGGACTACCCCAACC
  CCACCCTGGACCACTACAAGGAACCCTCTGCCATCTTCCAGCTGCCCTACGAGAAGGACTGCAAGAACAA
  AGTGTTCTGCATCGCTGAGATCCAGCTGACCACCAACATCTCCCAGCAGGAACTGGTGGTGGGCGTCACA
  AAGGAGGTGACCATGAACATCAGCCTGACTAACTCTGGAGAGGATTCCTACATGACAAACATGGCTCTCA
  ATTATCCCAGAAACTTACAGTTTAAGAAGATACAAAAGCCAGTATCTCCAGATGTTCAGTGTGATGACCC
  CAAGCCAGTTGCTTCTGTCCTGGTCATGAACTGCAAGATTGGTCACCCCATACTCAAGAGATCATCTGTG
  AATGTCTCAGTAACTTGGCAGCTAGAGGAGAGTGTCTTTCCAAATAGGACAGCAGATATCACTGTGACCA
  TCTCCAATTCCAATGAGAAGTCTTTGGCCAGAGAGACACGCAGCCTTCAATTCAGACATGCCTTCATTGC
  AGTTCTTTCCAGACCCTCAGTGATGTACATGAACACAAGCCAAAGCCCTTCTGACCACAAAGAATTCTTC
  TTCAATGTGCATGGGGAGAATCTTTTTGGAGCTGTATTCCAGTTGCAAATTTGTGTCCCCATCAAATTAC
  AAGATTTCCAGATTGTAAGAGTGAAGAATCTGACAAAGACTCAGGTATTTAAATGGGAGTCTGTGATTGC
  ATCGCTTTCCAAAGACTACAGATTCTCAAAGATATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172944
• Insert Size: 3117 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172944.2, NP_766532.1
• RefSeq Size: 3813 bp
• RefSeq ORF: 3117 bp
• Locus ID: 16407
• Cytogenetics: 11 45.22 cM
• Gene Summary: Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Mice expressing a null mutation of the alpha-E subunit gene exhibit a marked reduction in the numbers of intraepithelial lymphocytes in the gut and in the development of gut-associated lymphoid aggregates, supporting a specific role for this integrin in mediating retention of lymphocytes in the intestinal wall.[UniProtKB/Swiss-Prot Function]