Atp2a2 (NM_001110140) Mouse Untagged Clone

CAT#: MC223305

Atp2a2 (untagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp2a2
• Synonyms: 9530097L16Rik; D5Wsu150e; mKIAA4195; SERCA2; Serca2a; SERCA2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223305 representing NM_001110140
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGCTCACACAAAGACCGTGGAGGAGGTGCTGGGCCACTTCGGGGTCAACGAGAGCACGGGGC
  TGAGCTTGGAGCAGGTCAAGAAGCTCAAGGAGAGATGGGGCTCCAACGAATTGCCGGCTGAAGAAGGAAA
  AACCTTGCTGGAACTTGTGATTGAGCAGTTTGAAGACTTACTAGTTAGAATTTTACTGCTGGCAGCATGT
  ATATCTTTCGTTTTGGCTTGGTTCGAGGAAGGGGAAGAAACGATTACAGCCTTTGTAGAGCCGTTTGTAA
  TTCTGCTTATCTTGGTAGCCAATGCAATCGTGGGTGTGTGGCAGGAAAGAAATGCTGAAAATGCAATAGA
  AGCTCTTAAGGAATATGAGCCTGAAATGGGCAAAGTGTATCGACAGGACAGAAAGAGTGTGCAACGAATT
  AAAGCTAAAGACATAGTTCCTGGTGATATAGTGGAAATTGCTGTTGGTGACAAAGTTCCTGCTGATATTA
  GATTGACATCCATCAAGTCTACAACTCTAAGAGTCGACCAGTCAATTCTTACAGGTGAATCTGTCTCCGT
  CATCAAGCATACTGACCCTGTCCCTGACCCCCGAGCTGTTAATCAAGACAAAAAGAACATGCTCTTTTCT
  GGTACAAACATTGCTGCTGGGAAAGCTATGGGAGTGGTGGTGGCAACTGGAGTTAATACTGAGATCGGCA
  AGATCCGGGATGAAATGGTGGCAACAGAACAGGAGAGAACACCCCTACAGCAGAAGCTAGACGAGTTTGG
  GGAGCAGCTTTCCAAAGTTATCTCCCTCATTTGCATTGCAGTCTGGATCATCAACATTGGGCATTTCAAT
  GACCCAGTTCATGGTGGCTCCTGGATCAGGGGTGCCATCTACTACTTTAAGATTGCCGTGGCCCTGGCTG
  TTGCCGCAATCCCTGAGGGTCTGCCTGCTGTCATCACCACCTGCTTAGCTCTTGGAACTCGTAGGATGGC
  AAAGAAAAATGCTATCGTTCGAAGTCTGCCTTCTGTGGAGACCCTTGGTTGTACTTCTGTTATCTGCTCA
  GATAAGACAGGCACACTTACCACAAACCAGATGTCCGTGTGCAGGATGTTCATTCTGGACAAAGTAGAAG
  GTGACACTTGTTCCCTTAATGAGTTCAGCATAACTGGATCCACATATGCACCAATTGGAGAAGTGCAAAA
  GGATGATAAGCCAGTGAAGTGCCATCAGTATGACGGGCTTGTAGAGTTAGCCACCATCTGTGCTCTGTGT
  AATGACTCTGCTTTGGATTATAATGAGGCAAAGGGTGTGTATGAGAAAGTTGGAGAAGCTACCGAGACTG
  CTCTCACGTGCCTGGTGGAGAAGATGAATGTATTTGATACTGAGCTGAAGGGGCTTTCTAAAATAGAGCG
  TGCAAACGCCTGCAACTCGGTCATAAAGCAGCTGATGAAGAAGGAGTTCACTCTGGAGTTTTCACGGGAT
  AGAAAATCAATGTCCGTCTATTGTACCCCAAACAAGCCAAGCCGGACATCCATGAGCAAGATGTTTGTGA
  AGGGGGCTCCAGAAGGTGTCATCGATAGGTGCACCCACATCCGAGTTGGAAGTACCAAGGTCCCCATGAC
  TCCTGGTGTCAAACAGAAGATTATGTCTGTCATTCGGGAGTGGGGCAGTGGCAGCGACACGCTACGGTGC
  CTGGCTCTGGCCACTCATGACAACCCACTGAAGAGAGAGGAGATGCACCTGGAAGACTCTGCTAACTTCA
  TCAAATACGAGACCAACCTGACTTTCGTCGGCTGTGTGGGCATGCTGGATCCTCCCAGGATTGAAGTAGC
  CTCTTCTGTGAAGCTGTGCCGGCAAGCAGGCATCCGGGTCATCATGATCACTGGAGACAACAAAGGCACC
  GCTGTGGCCATCTGTCGCCGCATTGGCATCTTTGGGCAGGATGAGGATGTGACATCAAAGGCTTTTACAG
  GGCGAGAGTTTGATGAATTAAGCCCTTCAGCCCAGAGAGATGCCTGCTTAAATGCCCGCTGTTTTGCTCG
  AGTTGAACCTTCCCACAAGTCTAAGATTGTTGAGTTCCTTCAGTCCTTTGATGAGATCACAGCTATGACT
  GGTGATGGTGTGAATGATGCTCCTGCTCTGAAGAAATCTGAAATCGGGATTGCCATGGGCTCAGGGACTG
  CAGTGGCTAAGACTGCTTCTGAGATGGTCCTGGCAGATGACAACTTCTCCACCATTGTGGCTGCTGTTGA
  GGAGGGGCGAGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCATCCAACGTGGGGGAA
  GTGGTCTGTATCTTCCTGACGGCAGCCCTTGGGTTTCCTGAGGCTTTAATTCCTGTCCAGTTACTCTGGG
  TCAATCTGGTGACAGATGGTCTGCCTGCCACTGCGCTGGGGTTCAATCCTCCAGACCTGGACATCATGAA
  CAAACCCCCCCGGAACCCAAAAGAACCACTGATCAGCGGGTGGCTCTTTTTCCGTTACCTGGCTATTGGC
  TGTTATGTTGGCGCTGCCACCGTGGGTGCTGCTGCATGGTGGTTCATCGCTGCTGACGGCGGTCCAAGAG
  TCTCCTTCTACCAGCTGAGTCATTTCCTACAGTGTAAGGAGGACAACCCAGACTTCGATGGAGTGGATTG
  TGCAATCTTTGAGTCCCCATATCCAATGACAATGGCACTTTCTGTTCTAGTAACCATAGAGATGTGTAAT
  GCCCTCAACAGCTTGTCTGAAAACCAGTCTTTGCTGAGGATGCCCCCCTGGGAGAATATCTGGCTCGTGG
  GCTCCATCTGCTTGTCCATGTCACTTCACTTCTTGATCCTCTACGTGGAACCTTTGCCGCTCATTTTCCA
  GATCACACCGCTGAATCTGACCCAGTGGCTGATGGTGCTGAAAATCTCCTTGCCTGTGATCCTCATGGAT
  GAGACGCTCAAGTTTGTGGCCCGAAACTACCTGGAACAACCCGGTAAAGAGTGTGTGCAGCCTGCCACCA
  AATCTTCCTGCTCCCTGTCGGCATGCACCGATGGCATTTCCTGGCCGTTTGTGCTGCTCATTATGCCCCT
  GGTGGTCTGGGTCTACAGCACAGACACTAACTTTAGCGATATGTTCTGGTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110140
• Insert Size: 3135 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110140.3, NP_001103610.1
• RefSeq Size: 4565 bp
• RefSeq ORF: 3135 bp
• Locus ID: 11938
• UniProt ID: O55143
• Cytogenetics: 5 F
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform SERCA2A is involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (PubMed:23395171).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (b), also known as SERCA2B.