Tlr9 (NM_031178) Mouse Untagged Clone

CAT#: MC223287

Tlr9 (untagged) - Mouse toll-like receptor 9 (Tlr9), (10ug)


  "NM_031178" in other vectors (4)

Reconstitution Protocol

USD 1,349.00

In Stock*

Size
    • 10 ug

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Frequently bought together (2)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "Tlr9"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Tlr9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223287 representing NM_031178
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCTCCGTCGAAGGACTCTGCACCCCTTGTCCCTCCTGGTACAGGCTGCAGTGCTGGCTGAGACTC
TGGCCCTGGGTACCCTGCCTGCCTTCCTACCCTGTGAGCTGAAGCCTCATGGCCTGGTGGACTGCAATTG
GCTGTTCCTGAAGTCTGTACCCCGTTTCTCTGCGGCAGCATCCTGCTCCAACATCACCCGCCTCTCCTTG
ATCTCCAACCGTATCCACCACCTGCACAACTCTGACTTCGTCCACCTGTCCAACCTGCGGCAGCTGAACC
TCAAGTGGAACTGTCCACCCACTGGCCTTAGCCCCCTGCACTTCTCTTGCCACATGACCATTGAGCCCAG
AACCTTCCTGGCTATGCGTACACTGGAGGAGCTGAACTTGAGCTATAATGGTATCACCACTGTGCCCCGA
CTGCCCAGCTCCCTGGTGAATCTGAGCCTGAGCCACACCAACATCCTGGTTCTAGATGCTAACAGCCTCG
CCGGCCTATACAGCCTGCGCGTTCTCTTCATGGACGGGAACTGCTACTACAAGAACCCCTGCACAGGAGC
GGTGAAGGTGACCCCAGGCGCCCTCCTGGGCCTGAGCAATCTCACCCATCTGTCTCTGAAGTATAACAAC
CTCACAAAGGTGCCCCGCCAACTGCCCCCCAGCCTGGAGTACCTCCTGGTGTCCTATAACCTCATTGTCA
AGCTGGGGCCTGAAGACCTGGCCAATCTGACCTCCCTTCGAGTACTTGATGTGGGTGGGAATTGCCGTCG
CTGTGACCATGCCCCCAATCCCTGTATAGAATGTGGCCAAAAGTCCCTCCACCTGCACCCTGAGACCTTC
CATCACCTGAGCCATCTGGAAGGCCTGGTGCTGAAGGACAGCTCTCTCCATACACTGAACTCTTCCTGGT
TCCAAGGTCTGGTCAACCTCTCGGTGCTGGACCTAAGCGAGAACTTTCTCTATGAAAGCATCACCCACAC
CAATGCCTTTCAGAACCTAACCCGCCTGCGCAAGCTCAACCTGTCCTTCAATTACCGCAAGAAGGTATCC
TTTGCCCGCCTCCACCTGGCAAGTTCCTTTAAGAACCTGGTGTCACTGCAGGAGCTGAACATGAACGGCA
TCTTCTTCCGCTTGCTCAACAAGTACACGCTCAGATGGCTGGCCGATCTGCCCAAACTCCACACTCTGCA
TCTTCAAATGAACTTCATCAACCAGGCACAGCTCAGCATCTTTGGTACCTTCCGAGCCCTTCGCTTTGTG
GACTTGTCAGACAATCGCATCAGTGGGCCTTCAACGCTGTCAGAAGCCACCCCTGAAGAGGCAGATGATG
CAGAGCAGGAGGAGCTGTTGTCTGCGGATCCTCACCCAGCTCCGCTGAGCACCCCTGCTTCTAAGAACTT
CATGGACAGGTGTAAGAACTTCAAGTTCACCATGGACCTGTCTCGGAACAACCTGGTGACTATCAAGCCA
GAGATGTTTGTCAATCTCTCACGCCTCCAGTGTCTTAGCCTGAGCCACAACTCCATTGCACAGGCTGTCA
ATGGCTCTCAGTTCCTGCCGCTGACTAATCTGCAGGTGCTGGACCTGTCCCATAACAAACTGGACTTGTA
CCACTGGAAATCGTTCAGTGAGCTACCACAGTTGCAGGCCCTGGACCTGAGCTACAACAGCCAGCCCTTT
AGCATGAAGGGTATAGGCCACAATTTCAGTTTTGTGACCCATCTGTCCATGCTACAGAGCCTTAGCCTGG
CACACAATGACATTCATACCCGTGTGTCCTCACATCTCAACAGCAACTCAGTGAGGTTTCTTGACTTCAG
CGGCAACGGTATGGGCCGCATGTGGGATGAGGGGGGCCTTTATCTCCATTTCTTCCAAGGCCTGAGTGGC
CTGCTGAAGCTGGACCTGTCTCAAAATAACCTGCATATCCTCCGGCCCCAGAACCTTGACAACCTCCCCA
AGAGCCTGAAGCTGCTGAGCCTCCGAGACAACTACCTATCTTTCTTTAACTGGACCAGTCTGTCCTTCCT
ACCCAACCTGGAAGTCCTAGACCTGGCAGGCAACCAGCTAAAGGCCCTGACCAATGGCACCCTGCCTAAT
GGCACCCTCCTCCAGAAACTCGATGTCAGTAGCAACAGTATCGTCTCTGTGGTCCCAGCCTTCTTCGCTC
TGGCGGTCGAGCTGAAAGAGGTCAACCTCAGCCACAACATTCTCAAGACGGTGGATCGCTCCTGGTTTGG
GCCCATTGTGATGAACCTGACAGTTCTAGACGTGAGAAGCAACCCTCTGCACTGTGCCTGTGGGGCAGCC
TTCGTAGACTTACTGTTGGAGGTGCAGACCAAGGTGCCTGGCCTGGCTAATGGTGTGAAGTGTGGCAGCC
CCGGCCAGCTGCAGGGCCGTAGCATCTTCGCGCAGGACCTGCGGCTGTGCCTGGATGAGGTCCTCTCTTG
GGACTGCTTTGGCCTTTCACTCTTGGCTGTGGCCGTGGGCATGGTGGTGCCTATACTGCACCATCTCTGC
GGCTGGGACGTCTGGTACTGTTTTCATCTGTGCCTGGCATGGCTACCTTTGCTAGCCCGCAGCCGACGCA
GCGCCCAAACTCTCCCTTATGATGCCTTCGTGGTGTTCGATAAGGCACAGAGCGCAGTTGCCGACTGGGT
GTATAACGAGCTGCGGGTGCGGCTGGAGGAGCGGCGCGGCCGCCGAGCCCTACGCTTGTGTCTGGAGGAC
CGAGATTGGCTGCCTGGCCAGACGCTCTTCGAGAACCTCTGGGCTTCCATCTATGGGAGCCGCAAGACTC
TATTTGTGCTGGCCCACACGGACCGCGTCAGTGGCCTCCTGCGCACCAGCTTCCTGCTGGCTCAGCAGCG
CCTGTTGGAAGACCGCAAGGACGTGGTGGTGTTGGTGATCCTGCGTCCGGATGCCCACCGCTCCCGCTAT
GTGCGACTGCGCCAGCGTCTCTGCCGCCAGAGTGTGCTCTTCTGGCCCCAGCAGCCCAACGGGCAGGGGG
GCTTCTGGGCCCAGCTGAGTACAGCCCTGACTAGGGACAACCGCCACTTCTATAACCAGAACTTCTGCCG
GGGACCTACAGCAGAATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_031178
Insert Size 3099 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031178.2, NP_112455.2
RefSeq Size 3471 bp
RefSeq ORF 3099 bp
Locus ID 81897
UniProt ID Q9EQU3
Cytogenetics 9 F1
Gene Summary Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:18931679, PubMed:21402738, PubMed:14993594, PubMed:17474149, PubMed:25686612, PubMed:18820679). Plays a role in defense against systemic mouse cytomegalovirus infection (PubMed:14993594). Controls lymphocyte response to Helicobacter infection (PubMed:17474149). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (By similarity).[UniProtKB/Swiss-Prot Function]

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