Tacc2 (NM_021314) Mouse Untagged Clone

CAT#: MC223276

Tacc2 (untagged) - Mouse transforming, acidic coiled-coil containing protein 2 (Tacc2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tacc2
• Synonyms: mKIAA4180
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223276 representing NM_021314
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGGTCGCAGTCCCTGCAGCCAGCCCCAGCCAGTGACCTGAACCTGGAGGTTTCTGAGGCCATGA
  GTTCCGACTCTGAGGAAGCCTTTGAGACCCCCGAGTCAACGACCCCTGTCAAAGCTCCACCAGCCCCTCC
  CCCTCCACCCCCTGAAGTCACCCCAGAGCCTGAGGTCATCGATCCACCAGCCCCAGAAGAACCAGGATGC
  ATTTCTGAGCCACCGGTTGTGGTTCCCGATGGCCCTCGCAGCAGCGAGTCCGTGGAAGGAAGCCCTTTCC
  GTCCTTCCCACTCCTCCTCGGCGGTGTTCGATGAAGACAAGCCGATAGCCAGCAGCGGGACTTACAACTT
  AGACTTCGACAGCATCGAGCTGGTGGATAACTTCCAGAGCTTGGAGCCGTGCTCTGCCGACTCTAAGGGT
  CAGGAGTGTAAGGTGAGCACGCGGAGGAAATCCACCGAGTCCGTGCCACCCTCCAAGTCCACGCTGTCCC
  GTTCGCTTAGCCTACAAGCCAGTGACTTTGACGGTGCTTCTTGCCCCGGCAGCCCTGAAGCTGGGACCCT
  TACCACAGATGCATGTGGCACAGGATCCAACAGTGCTTCCAGCACCCTTAAGCGAACTAAAAAAACACGG
  CCACCTTCCTTGAAAAAGAAGCAAGCCACCAAGAAACCCACAGAAACCCCCCCAGTGAAGGAGACCCAAC
  AGGAACCAGGTGAAGAGAGTCCAGTCCCCAGTGAGGAACACTTAGCACCAGAGACAAAGACGGAGTCGGC
  CACACCTGAGGGCGCTGGTTGCACCTTGTCAGACGATACACCCCTGGAGTCCCCTGCTGTCCCCACAGCC
  ACCTGTCCTCTGACTTTGGAGAGTGCAGAAGACGTTAGCCCCCTGGTTTCTGGAGGTGGCAGAGTGCAGA
  ACTCACCCCCAGTTGGGAGGAAATCAGTGCCTCTTACCACAGCCTCTGAGGCGGTGGAGGTGACCCTGTC
  GGACAGTGGGGGGCAAGAGGACTTGCCTGCCAAGGGGTTGTCAGTGAGGCTGGAGTTTGACTATTCTGAG
  GACAAGGGAAGTTGGGAGAGTCAGCAGGAAAACGCCCCTCCCACCAAAAAGATAGGCAAGAAGCCAGTTG
  CCAAAATGCCCCTAAGGAGGCCAAAGATGAAAAAGACCCCCGAGAAACTTGACAACACTCCTGCCTCACC
  TCCAAGGTCCCCTACTGAACCCAGTGACACCCCTATTGCTAAAGGTACCTACACCTTTGATATAGACAAG
  TGGGATGACCCCAATTTTAACCCTTTTTCCTCCACCTCAAAAATGCAAGAGTCTCCCAAACTGTCCCAAC
  AATCGTACAACTTTGACCCTGACGCCTGTGAAGAGTCCCTTGACCCCTTTAAGGCATCCTCTAAGACCCC
  CAGCTCACCTTCTAAGTCCCCAGCCTCTTTTGAGATCCCAGCCAGCACCACGGAAGCGGATGGGGATGGG
  TTGAATAAACCTGCCAAGAAGAAGAAGACTCCACTAAAGACGATGGTTGAAGATGTGATGTCTGTGTGTT
  CTCTGTTTGACACGTTTAGGGTGAAGAAGTCTCCAAAGCGGTCTCCTCTGTCTGACCCGCCTTCTCAGGA
  CCCCACTCCAGCTGCCACGCCGGAAGCACCCTCAGCCATCTCTACAGTGGTCCACGCCACAGATGAGGAG
  AAGCTGGCAGTCACTAGCCAGAAGTGGACGTGTATGACGGTGGACTTGGATGCTGACAAACAGGACTTCC
  CTCAGCCCTCGGACCTGTCTAACTTTGTAAATGAGACCAAATTCAATTCACCCTCAGAGGAGCTGGACTA
  CAGAAACTCCTATGAAATTGAATACATGGAAAAGCTTGGCTCCTCCTTACCTCAGGACGATGACACTCCG
  AAGAAGCAGGCCTTGTACCTTATGTTTGACACTCCTCAGGAGAGCCCCGTCAAGTCTCCTCCAGTCCGGA
  TGTCAGATTCCCCAACTCCATGTTCAGGGTCGAGTTTTGAGGACACCGAAGCCCTTGTGAATGCAGCAAC
  AAAGCTCCAGCATCCAGTCGCAAGAGGTCTGCCCTCCAGTCAGGAGCCTCTCTTGCAGGTGCCTGAGAAG
  CCCTCCCAGAAGGAGCTGGAGGCCATGGCCTTGGGCACCCCAGCAGAAGCAATTGAAATCACAGCTCCCG
  AGGGTGCCTTTGCTTCTGCAGATACCCTCCTCAGCAGGCTGGCCCATCCCGCCTCTCTCTGTGGTGCACT
  TGGCTATCTGGAGCCTGACTTAGCAGAAAAGAACCCCCCAGTATTTGCCCAGAAACTTCAGGAGGAGTTA
  GAGTTTGCTGTCATGCGGATAGAAGCCCTGAAGCTGGCCAGGCAGATCGCCCTGGCTTCCCGCAGCCGCC
  AGGACACCAAGAGGGAAGCTGCTCACCCACCAGATGTCTCCATCTCCAAAACTGCCTTGTACTCCCGCAT
  CGGGTCCACCGAGGTGGAGAAACCCCCAGGCCTTCTGTTCCAGCAGCCAGACCTGGACTCTGCACTCCAG
  GTTGCCAGAGCAGAGGTCATCGCCAAGGAGAGAGAAGTCTCAGAGTGGAGGGATAAATATGAAGAAAGCC
  GACGGGAAGTGGTGGAAATGAGGAAAATTGTGGCCGAATATGAGAAGACCATTGCACAGATGATCGAGGA
  CGAACAGAGAGAAAAATCCATCTCCCACCAAACTGTACAGCAGCTGGTCCTGGAGAAGGAGCAAGCCCTG
  GCTGACCTGAACTCTGTGGAAAAGTCTCTGGCTGACCTCTTCAGGAGATACGAAAAGATGAAGGAGGTCC
  TGGAAGGTTTTCGAAAGAATGAAGAGGTGTTGAAGAAATGTGCGCAGGAGTACCTATCCCGAGTGAAGAA
  AGAGGAACAGAGGTACCAGGCCCTGAAGGTGCACGCCGAAGAGAAACTGGACAGAGCCAATGCAGAGATT
  GCCCAGGTTCGCGGCAAGGCCCAGCAAGAGCAAGCAGCCTACCAGGCTAGCCTGCGGAAGGAGCAGCTTC
  GAGTGGATGCTCTGGAAAGAACGCTGGAGCAGAAGAATAAAGAGATAGAAGAACTCACCAAGATTTGTGA
  CGAGCTGATCGCCAAGATGGGAAAAAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021314
• Insert Size: 3111 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021314.4, NP_067289.2
• RefSeq Size: 3940 bp
• RefSeq ORF: 3111 bp
• Locus ID: 57752
• UniProt ID: Q9JJG0
• Cytogenetics: 7 F3
• Gene Summary: This gene encodes a member of the transforming, acidic coiled-coil (TACC) family of proteins. Members of this family are centrosomal proteins that interact with microtubules and tubulin. TACC proteins are thought to be involved in centrosome/mitotic spindle dynamics and gene regulation. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region compared to variant 3. These differences cause translation initiation at a downstream start codon and result in an isoform (b) with a shorter N-terminus compared to isoform c.