Itga2b (NM_010575) Mouse Untagged Clone

CAT#: MC223271

Itga2b (untagged) - Mouse integrin alpha 2b (Itga2b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itga2b
• Synonyms: AI172977; alphaIIb; CD41; CD41B; GpIIb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223271 representing NM_010575
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGCCTCCTGCGCATGGCATTCCCTGTGGCTTCTGCAGTGGACACCACTGTTCTTGGGTCCTA
  GTGCTGTTCCTCCAGTCTGGGCCTTGAACCTGGACTCTGAGAAGTTCTCCGTCTATGCAGGTCCCAATGG
  CAGCCACTTTGGCTTCTCAGTGGACTTTCATAAGGACAAACATGGAAGCGTGTCCATCGTGGTGGGCGCC
  CCCAGGGCCCTAAACGCAAGTCAAGAGGAGACTGGAGCCGTATTCCTGTGCCCCTGGAAGGCCAATGGTG
  GCAAGTGCAACCCGTTGCTCTTCGACCTCAGGGATGAGACACGAAACCTAGGCTTCCAAATTTTCCAAAC
  CTTCAAGACCGGGCAAGGACTTGGGGCGTCGGTCGTCAGCTGGAATGACGTCATTGTGGCCTGTGCCCCC
  TGGCAGCACTGGAATGTCCTGGAAAAGCGCGATGAGGCAGAGAAGACTCCGGTAGGAGGCTGCTTCTTGG
  CTCAGCTCCAAAGCGGGGGCCGCGCAGAGTACTCGCCCTGTCGCGCCAACACCATGAGCTCCGTTTACGC
  AGAGAGTTTTCGCGGAGACAAGCGATACTGTGAAGCAGGCTTCAGCTTGGCGGTGACCCAGGCTGGGGAG
  CTAGTGCTTGGAGCCCCTGGAGGCTACTTTTTTTTAGGTCTCCTGGCTCGCGTTCCAATTGAGAACATCA
  TCTCCAGCTACCGCCCGGGTACCCTTTTGTGGCATGTTTCCAACCAGCGCTTCACCTACGACAACAGCAA
  CCCAGTGTTTTTCGATGGTTACCGGGGATATTCGGTAGCCGTGGGCGAGTTTGATGGGGATCCGAGCACT
  ACAGAGTACGTATCGGGTGCCCCCACTTGGAGCTGGACCTTGGGAGCGGTGGAAATTTTGGACTCCTACT
  ACCAGCCCCTGCACCGGCTTCATGGAGAACAGATGGCTTCATATTTCGGGCACTCGGTGGCAGTCACTGA
  CGTCAACGGGGACGGGAGGCATGACCTACTGGTGGGGGCTCCATTGTATATGGAGAGCAGGGCAGACCGC
  AAGCTGGCCGAGGTGGGCCGCGTTTATTTGTTTCTGCAGCCTAAGGGCCCCCAAGCTCTGAGCACACCCA
  CTCTCCTGCTGACTGGCACCCAGCTCTATGGGAGATTTGGATCTGCCATTGCACCCCTGGGTGACCTCAA
  CCGAGACGGCTATAATGATATTGCTGTGGCTGCCCCCTATGGGGGTCCCAGTGGTCAGGGCCAAGTGCTG
  ATATTCCTGGGTCAGAGTGAAGGGCTGAGTCCACGCCCCTCCCAGGTTCTGGACAGCCCCTTCCCCACAG
  GCTCTGGCTTTGGCTTCTCCCTTCGTGGTGCTGTAGACATCGATGACAATGGATACCCAGACCTGATTGT
  GGGAGCATATGGAGCTAGCAAGGTGGCTGTGTACAGAGCTCAGCCTGTGGTGATGGCCACTGTCCAACTG
  ATGGTTCAAGACTCCCTGAATCCAACACTGAAGAACTGTGTCCTGGATCAGACCAAGACACCAGTCAGCT
  GCTTCAACATCCAGATGTGTGTGGGAGCCACAGGACACAACATTCCTCAGAAGCTGCATCTAAAGGCAGA
  GCTGCAGCTGGACTTACAGAAGCCCCGTCAGGGCCGCCGGGTGCTCCTGCTGGCATCCCAACAGGCGAGC
  CTCACCCTGAGCCTGGACCTGGGCGGAAGAGACAAGCCTATCTGCCACACCACGGGGGCCTTCCTTCGGG
  ATGAGGCCGACTTCCGGGACAAGCTGAGCCCAATTGTGCTAAGCCTCAACGTGTCGCTGCCCCCAGAAGA
  GACTGGAGGAGCCCCTGCCGTGGTATTGCATGGAGAAACCCATGTCCAGGAGCAGACACGGATCATCCTG
  GATTGTGGGGAAGACGACCTGTGTGTGCCACAGCTCCGGCTCACAGCTACTGCGGGGGACTCCCCACTCC
  TAATCGGTGCTGACAATGTGTTGGAGCTGAAGATTGAAGCAGCCAATGATGGTGAGGGAGCCTATGAAGC
  GGAGCTGGCTGTGCATCTGCCTCCAGGTGCCCACTACATGCGGGCTCTCAGCAACATTGAGGGCTTTGAG
  AGGCTTGTCTGCACTCAGAAGAAAGAGAATGAGTCCAGGGTGGCCCTCTGTGAGCTGGGCAACCCCATGA
  AAAAGGACACCCGGATAGGAATCACAATGCTGGTGAGTGTGGAGAACCTGGAAGAAGCTGGCGAGTCCGT
  GTCCTTCCAGCTGCAGGTCAGGAGCAAGAACAGCCAGAATCCAAACAGTAAGGTCGTGATGTTGCCTGTG
  GCAATCCAAGCTGAAGCCACAGTGGAGCTTCGAGGGAATTCCTTCCCTGCCTCCCTGGTGGTGGCAGCAG
  AAGAAGGTGACAGGGAGCAGGAAGACTTGGACAGATGGGTCTCCAGGCTGGAGCACACCTATGAGCTCCA
  CAACATTGGCCCTGGCACTGTGAATGGCCTCAGACTTCTCATCCACATTCCTGGCCAGTCCCAGCCCTCG
  GATCTGCTCTACATCCTGGATGTGCAGCCGCAGGGAGGTCTCCTTTGCTCCACACAGCCATCTCCCAAGG
  TGGACTGGAAACTATCCACGCCCAGCCCTTCTTCCATTCGCCCCGTCCATCACCAGCGTGAGCGCAGACA
  GGCATTCCTGCAGGGGCCCAAGCCAGGGCAGCAGGACCCAGTTCTGGTGAGCTGCGACGGCTCAGCGTCC
  TGTACGGTGGTGGAGTGTGAGCTGCGGGAGATGGTGCGCGGGCAGCGGGCCATGGTTACTGTGCAGGTCA
  TGCTGGGGCTGTCCAGCCTCCGCCAGAGGCCGCAGGAGCAGTTTGTGCTGCAGTCGCACGCCTGGTTCAA
  CGTCTCCTCCCTACCTTACTCGGTGCCGGTGGTCAGCTTGCCCAGTGGGCAAGCTCGGGTGCAGACACAG
  CTGCTTCGGGCCTTGGAGGAGAGGGCCATTCCTGTCTGGTGGGTGCTGGTGGGCGTGCTGGGCGGTCTGC
  TGCTGCTGACCCTGCTAGTTTTGGCCATGTGGAAGGCTGGCTTCTTCAAGCGGAATCGACCGCCTCTGGA
  GGAAGATGAAGAGGAAGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010575
• Insert Size: 3102 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010575.2, NP_034705.2
• RefSeq Size: 3437 bp
• RefSeq ORF: 3102 bp
• Locus ID: 16399
• UniProt ID: Q9QUM0
• Cytogenetics: 11 66.29 cM
• Gene Summary: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.[UniProtKB/Swiss-Prot Function]