Phf8 (NM_001113354) Mouse Untagged Clone

CAT#: MC223259

Phf8 (untagged) - Mouse PHD finger protein 8 (Phf8), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phf8
• Synonyms: 9830141C09Rik; mKIAA1111
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223259 representing NM_001113354
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGTGCCTGTGTATTGCCTCTGTCGACTGCCTTATGATGTGACCCGCTTCATGATCGAGTGTG
  ACATGTGCCAGGACTGGTTTCACGGCAGTTGTGTTGGTGTTGAGGAGGAGAAGGCTGCTGATATTGATCT
  TTACCACTGCCCTAACTGTGAGGTCTTACATGGACCTTCCATTATGAAAAAACGTCGTGGATCTTCCAAA
  GGACATGATAATCACAAGGGGAAGCCACTGAAGACTGGAAGCTCTATGTTTATCCGAGAACTTCGGGGCA
  GAACTTTTGACAGCTCAGATGAAGTGATTCTGAAGCCCACTGGAAGTCAGCTGACTGTGGAATTCTTGGA
  AGAGAATAGCTTCAGCGTGCCTATCCTTGTCTTGAAGAAGGATGGGTTGGGGATGACATTACCCTCTCCA
  TCATTCACTGTGAGGGATGTGGAACACTATGTTGGTTCTGACAAAGAGATTGATGTGATTGATGTGGCCC
  GCCAGGCTGACTGCAAGATGAAACTCGGTGATTTTGTCAAATACTATTACAGTGGGAAGAGGGAAAAAGT
  CCTCAATGTCATTAGTTTGGAATTCTCCGATACCAGGCTTTCAAACCTCGTGGAAACACCCAGGATTGTT
  CGCAAGCTGTCATGGGTGGAGAACTTGTGGCCAGAGGAATGTGTCTTTGAGAGACCCAATGTGCAGAAGT
  ACTGCCTCATGAGTGTGCGGGATAGCTATACAGATTTTCACATTGACTTTGGTGGGACCTCAGTTTGGTA
  CCATGTGCTTAAGGGTGAGAAGATCTTCTACCTGATCCGCCCAACAAATGCTAATCTGACTCTCTTTGAG
  TGCTGGAGTAGCTCCTCCAATCAGAACGAGATGTTCTTTGGTGACCAAGTGGAAAAGTGCTACAAGTGTT
  CTGTGAAGCAAGGACAAACACTGTTTATTCCTACAGGATGGATACATGCTGTGTTAACACCCGTGGACTG
  CTTAGCATTCGGAGGGAACTTCTTACACAGTCTTAACATTGAAATGCAACTCAAGGCTTATGAAATTGAG
  AAGAGGCTGAGCACAGCAGACCTTTTCAAGTTTCCCAACTTCGAGACCATCTGTTGGTATGTGGGAAAAC
  ATATTCTGGACATCTTTCGAGGCTTACGAGAAAATAGAAGACACCCTGCCTCCTACCTGGTCCATGGTGG
  TAAAGCTCTGAACTTGGCATTTAGAGCTTGGACAAAGAAAGAAGCTTTGCCAGACCACGAGGATGAGATC
  CCAGAGACAGTGCGGACTGTACAGCTCATTAAAGATCTGGCTAGGGAGATCCGTCTGGTTGAAGACATCT
  TCCAACAGAACGTTGGGAAGACGAGCAATATCTTTGGGCTGCAGAGGATCTTCCCAGCTGGCTCCATCCC
  CTTAACCAAGCCAGCCCATTCCACTTCAGTATCCATGTCCAAGCTGTCACTGCCCTCCAAAAATGGTTCA
  AAGAAGAAAGGCCTGAAGCCCAAGGATATCTTCAAGAAGGCAGAGCGAAAGGGCAAGCAGAGTTCAGCCT
  TGGGGCCTGCTGGCCAGTTGAGCTATAATCTCATGGACCCATACAGTCATCAGGCACTGAAGACAGGCCC
  TTCCCAGAAAGCAAAGTTCAACATGAGTGGTACCAGCTTGAATGATTCAGATGATGACTCAGCAGACATG
  GACCTTGATGGCAGTGAGAACCCTCTGGCCCTGTTGATGGCTAATGGCAGTACGAAGAGGATGAAGAGTG
  TATCCAAATCTCGGAGAGCCAAAATTGCAAAGAAGGTAGACAGTGCAAGACTGGTAGCAGAACAGGTCAT
  GGGAGATGAATTTGACTTGGATTCAGATGATGAGCTGCAGATTGACGAGAGATTGGGAAAGGAAAAGGCG
  AACCTGTTAATAAGATCAAAATTTCCCCGGAAGTTGCCCCGTGCAAAACCTTGCTCTGACCCCAACCGAA
  TTCGTGAACCTGGAGAAGTTGAGTTTGACATTGAGGAGGACTATACCACAGATGAGGACATGGTGGAAGG
  GGTTGAAAGCAAGCTTGGGAATGGGAGTGGAGCCGGTGGAATTCTTGATCTACTTAAGGCCAGCAGGCAG
  GTGGGGGGACCTGACTATGCTGCCCTCACTGAGGCCCCAGCCTCCCCCAGCACTCAAGAGGCCATCCAGG
  GCATGTTGTGTATGGCCAACCTGCAGTCCTCATCATCCTCACCAGCTACCTCCAGTCTGCAGGCTTGGTG
  GACTGGAGGGCAAGAAAGAAGCAGCGGGAGCTCCAGCAGTGGCCTGGGCACTGTGTCTAGTAGTCCTGCT
  TCCCAGCGCACCCCAGGGAAGCGGCCCATCAAGAGGCCAGCATACTGGAAAAACGAGAGTGAAGAGGAGG
  AGAATGCCAGTCTTGATGAGCAAGACAGCTTGGGAGCATGCTTCAAGGATGCTGAGTATATCTATCCATC
  TCTGGAGTCTGATGATGATGACCCTGCTTTGAAATCTCGACCCAAGAAAAAGAAGAATTCAGATGATGCT
  CCATGGAGTCCTAAAGCCCGTGTGACTCCAACCCTACCCAAGCAGGACCGTCCTGTTCGTGAGGGGACCA
  GAGTTGCCTCCATTGAGACAGGCTTGGCTGCAGCAGCTGCAAAGCTTGCCCAGCAGGAGCTACAAAAGGC
  CCAAAAGAAGAAATATATCAAGAAGAAGCCTTTGCTGAAGGAGGTAGAACAGCCGCGCCCTCAAGATTCC
  AATCCCATCATGACAATGCCTGCACCAACTGTGGCTACAACACCCCAGCCTGACACCTCCTCCTCACCCC
  AGCCACCTCCTGAGCCTAAACAAGAGGCTCTGTCAGGAAGTCTTGCTGACCATGAGTACACTGCTCGTCC
  CAATGCCTTTGGCATGGCTCAGGCAAATCGCAGCACCACACCCATGGCCCCTGGAGTCTTCCTCACCCAG
  CGGCGCCCTTCAGTTGGTTCCCAGAGCAGTCAGGCAGGACAAGGAAAGCGTCCTAAAAAGGGCCTGGCCA
  CAGCAAAGCAGAGACTCGGCCGCATCCTGAAAATCCACAGAAATGGCAAGTTACTTCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113354
• Insert Size: 3072 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113354.1, NP_001106825.1
• RefSeq Size: 6359 bp
• RefSeq ORF: 3072 bp
• Locus ID: 320595
• UniProt ID: Q80TJ7
• Cytogenetics: X F3
• Gene Summary: Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.[UniProtKB/Swiss-Prot Function]