Myo1g (NM_178440) Mouse Untagged Clone

CAT#: MC223256

Myo1g (untagged) - Mouse myosin IG (Myo1g), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo1g
• Synonyms: E430002D17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223256 representing NM_178440
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGCAGTGGGCAGGATGGAGGACGAAGAAGGCCCTGAGTATGGGAAACCAGACTTCGTGCTTTTGG
  ATCAACTGACCATGGAGGACTTCATGAAGAACCTAGAGCTCAGGTTTGAGAAGGGCCGTATCTATACCTA
  CATTGGTGAGGTGCTCGTATCCGTGAACCCCTACCAGGAACTGCCATTGTATGGGCCAGAGGCCATTGCC
  AAGTACCAGGGCCGCGAGCTCTATGAGCGACCACCTCATCTTTACGCCGTGGCCAATGCTGCTTACAAGG
  CAATGAAGCGCAGATCCAGGGACACCTGCATCGTCATCTCAGGGGAGAGTGGGGCAGGGAAGACAGAAGC
  CAGCAAGCACATCATGCAGTACATTGCTGCTGTCACCAACCCAAGCCAGAGGGCTGAGGTGGAGAGGGTG
  AAGAATGTGCTCCTCAAGTCCACCTGTGTGCTCGAAGCCTTTGGCAATGCCCGCACCAATCGCAACCACA
  ACTCCAGCCGCTTTGGCAAGTACATGGACATCAACTTCGACTTCAAGGGGGATCCTGTTGGTGGACACAT
  CCACAGCTACCTGCTGGAGAAGTCTAGGGTTCTCAAGCAACATGTAGGTGAGAGGAACTTCCATGCCTTC
  TACCAGTTGCTTCGAGGCAGTGAGGACCAAGAGCTGCAAGGACTGCATCTGGAAAGAAATCCTGCTGTGT
  ATAATTTCACGCGTCAGGGAGCTGGGCTCAACATGGGTGTGCACAATGCCTTGGACAGTGATGAGAAGAG
  CCACCAAGGAGTGATGGAGGCCATGAGGATCATCGGCTTCAGTCCTGACGAGGTGGAGTCCATCCATCGC
  ATCCTTGCCGCCATATTACACCTGGGAAACATCGAGTTTGTGGAGACAGAGGAAAATGGACCACAGAAAG
  GAGGCCTGGAAGTGGCTGATGAGGCCCTGGTAGGATATGTGGCCAAGCTGACAGCCACTCCCAGAGACCT
  TGTTCTGCGAACCCTGCTGGCTCGAACAGTGGCTTCAGGAGGCCGAGAAGTCATTGAGAAGAGCCACACC
  GTGGCTGAGGCCAGCTATGCCCGGGATGCCTGTGCAAAGGCGATGTACCAGCGACTGTTTGAGTGGGTTG
  TGAACAAGATCAATAGCATCATGGAACCCCGAAACCGAGACCCTCGGTGTGATGGCAAGGATACTGTCAT
  TGGGGTGCTGGACATTTACGGCTTTGAGGTGTTCCCTGTCAACAGCTTTGAGCAGTTCTGCATCAACTAC
  TGCAACGAGAAGCTGCAGCAGCTCTTTATCCAGCTTATCCTGAAGCAAGAGCAGGAGGAGTATGAGCGAG
  AGGGCATCGCCTGGCAGACCATCGAGTACTTCAACAACGCTACCATTGTGGAACTTGTAGAGCAGCCCCA
  CAGAGGCATCCTGGCTGTGTTAGATGAAGCCTGCAGCACGGCAGGCCCCATCACTGACCGAATCTTCCTG
  CAGACCCTGGACACACACCACCGCCACCACCCACACTATTCCAGCCGCCAGCTTTGCCCTACGGACAAGA
  CCATGGAGTTTGGCCGAGACTTCCAGATCAAACACTATGCAGGCGATGTCACGTACTCTGTGGAAGGCTT
  CATTGACAAGAATAGAGACTCTCTCTTCCAGGACTTCAAACGGCTGCTGTACAATAGTGTGGATCCCACC
  TTGCGAGCCATGTGGCCTGACGGGCAACAGGACATCACGGAAGTGACCAAGCGTCCCCTGACAGCCGGCA
  CACTCTTTAAGAATTCCATGGTTGCCCTGGTGGAAAACTTGGCTTCCAAGGAACCCTTCTATGTCCGCTG
  CATCAAACCCAACGAAGACAAGGTGGCTGGGCGGCTCGATGAAGCCCACTGTCGTCACCAGGTCGAATAC
  CTGGGACTATTGGAGAATGTGAGGGTCCGCAGGGCTGGCTTTGCTTCCCGGCAGCCCTACCCTCGATTCC
  TGCTCAGGTACAAGATGACCTGTGAGTACACGTGGCCCAACCACCTGCTGGGCTCTGACCGGGATGCGGT
  GAGCGCCCTGCTGGAACAGCATGGGCTGCAAGGGGATGTGGCCTTTGGCCACAGCAAGCTGTTCATCCGA
  TCCCCAAGGACGCTGGTCACTCTGGAGCAGAGCCGAGCTCGCCTGATTCCCATCATTGTGTTATTGCTGC
  AGAAGGCTTGGCGGGGCACCCTGGCTAGGTGGCACTGCCGGCGACTAAGGGCCATCTACACCATCATGCG
  CTGGTTCCGGAGGCACAAGGTGCGTGCTCACCTGATTGAACTACAGCGCCGGTTCCAGGCTGCACGGCAG
  CCCCCACTCTATGGCCGTGACCTTGTGTGGCCCACACCTCCTGCTGTGCTGCAGCCCTTCCAGGACACTT
  GCCGTGTTCTCTTCAGCAGGTGGCGGGCACGGCAGTTAGTGAAGAACATCCCTCCTTCAGACATGATCCA
  GATCAAGGCCAAGGTGGCTGCTATGGGGGCCTTGCAAGGATTGCGGCAGGACTGGGGTTGCCAGCGGGCC
  TGGGCCCGAGACTACCTGTCCTCTGACACTGACAACCCCACAGCTTCCCATCTGTTTGCTGAGCAACTAA
  AGGCACTTCGGGAGAAAGATGGCTTTGGCTCTGTGCTTTTCTCCAGCCATGTGCGCAAGGTGAATCGCTT
  CCGCAAGAGCCGGGACCGGGCCCTTCTGCTCACAGATCGGTATCTGTACAAGCTGGAGCCTGGACGACAG
  TACCGGGTGATGCGGGCTGTGCCTCTGGAGGCGGTGACAGGGCTGAGTGTGACCAGTGGAAGAGATCAGC
  TGGTGGTGTTACATGCCCAAGGCTATGATGATCTTGTAGTGTGTCTACACCGTTCCCAACCACCACTGGA
  CAATCGAATTGGGGAGCTGGTGGGCATGCTGGCTGCACACTGCCAGGGAGAGGGACGAACTCTGGAGGTC
  CGTGTCTCTGACTGCATCCCACTGAGCCAGCGTGGTGCCCGGCGCCTCATTTCTGTGGAGCCCAGGCCAG
  AGCAGCCTGAGCCAGATTTCCAAAGCAGCCGTAGCACCTTTACCCTCCTCTGGCCAAGCCACTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_178440
• Insert Size: 3075 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178440.4, NP_848534.2
• RefSeq Size: 3307 bp
• RefSeq ORF: 3075 bp
• Locus ID: 246177
• UniProt ID: Q5SUA5
• Cytogenetics: 11 A1
• Gene Summary: Unconventional myosin required during immune response for detection of rare antigen-presenting cells by regulating T-cell migration (PubMed:25083865). Unconventional myosins are actin-based motor molecules with ATPase activity and serve in intracellular movements. Acts as a regulator of T-cell migration by generating membrane tension, enforcing cell-intrinsic meandering search, thereby enhancing detection of rare antigens during lymph-node surveillance, enabling pathogen eradication (PubMed:25083865). Also required in B-cells, where it regulates different membrane/cytoskeleton-dependent processes (PubMed:24310084). Involved in Fc-gamma receptor (Fc-gamma-R) phagocytosis (PubMed:23038771).[UniProtKB/Swiss-Prot Function]