Psd (NM_028627) Mouse Untagged Clone

CAT#: MC223241

Psd (untagged) - Mouse pleckstrin and Sec7 domain containing (Psd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Psd
• Synonyms: 1110007H17Rik; A930015K15; Efa6; Efa6a; mKIAA2011; Psdl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223241 representing NM_028627
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGGGTGCCATGCGCTTCTGCTCAGAAGGCGACTGTGCCATCTCCCCACCACGATGTCCTCGTC
  GCTGGCTCCCCGAAGGCCCAGTGCCCCAAAGTCCCCCAGCCAGCATGTATGGCAGCACAGGATCCCTTAT
  ACGACGAGTGGTGGGCCCAGGTCCTCGAGGCCGGGATCTGGGACGTGTGACAGCACCTTGTACCCCTCTG
  CGAGCCCCTCCCTCACCCCATATAGCTCCCTCACCCTGGGGACCCTCTTCACCCACTGGGCAGCCTCCAC
  CAGGGGCACAGAGTTCTGTGGTCATCTTCCGTTTTGTGGAGAAGGCCAGTGTGAGGCCACTGAATGGCCT
  ACCTGCTTCTGGAGGCCTGAGTCGGAGCTGGGACCTGGGCGGGATTTCTGCCCCCAGGCCCACCCCAGCC
  CTTGGACCTGGCTGCAACCGGAAGTTGCGGCTAGAGGCATCCACATCAGACCCACTCCCAGCTGGAGGAG
  GCTCTGTTCTTCCTGGCAGCAGGGACCCCTCACGTGGACCATTGGTCCCACCCCAGATCGGGGCAGATGG
  GCTTTACTCCTCCCTCCCCAATGGGCTGGGGGGGACCCCTGAGCACCTGGCCATGCACTTCCGAGGACCT
  GCAGACACCGGATTCCTAAACCAGGGGGACACCTGGTCCTCCCCCCGGGAAGTCTCCTCTCATGCCCAGA
  GAATCGCTCGAGCCAAGTGGGAATTCTTCTATGGCTCCTTGGACGCCCCCAGCTCAGGTGCTAAGCCCCC
  AGAGCAGGTCCTCCCATCACGTGGGGTGGGCTCCAAGCAAGGCTCTGGGGTGGCTGTGGGGCGAGCAGCC
  AAGTACTCCGAGACAGACCTGGACAAGGTGCCCCTGAGGTGCTACCGAGAGACTGACATCGATGAGGTGC
  TGGCTGAGCGGGAGGAGGCCGACTCTGCCATTGAGAGTCAGCCCAGCTCTGAGGGCCCCCATGGCACTGC
  CCAACCACCTGCCTCACGACCTAGCCCATGCCCTGGCCCTTCTTCCAGTCTGGGCAGTGGCAACGAAGAT
  GATGAGGCAGGAGGTGAAGAGGATGTGGATGATGAGGTGTTCGAGGCCTCGGAGGGGGCCCGGCCAGGGG
  ACCACATGCCTCACTCTGGGCTTCTCAAGTCACCAGTACCCTTCCTGCTTGGGACCAGCCCCTCAGCGGA
  TGGGCCTGACTCCTTCAGTTGTGTGTTTGAAGCTATCTTGGAGTCACACCGGGCCAAGGGCACCTCCTAT
  AGTAGCCTTGCCTCCCTGGAGGCACTGGCCTCACCTGGCCCAACCCAGAGCCCCTTCTTCACCTTTGAGA
  TGCCTCCCCAGCCCCCAGCTCCCAGGCCTGACCCACCTGCACCTGCCCCACTTGCCCCTCTTGAACCTGA
  TTCTGGCACCAGCTCTGCTGCTGATGGTCCTTGGACCCAGAGAAGGGAGGTGGAAGAGTCAGATGCTGGG
  GCCACGCTGGCCCCAAGGAAGGAGCTTCCTAGTCCCTCCCACTCTGAGGATAGCTTTGGGCTGGGGGCAG
  CACCCCTAGGCAGTGAACCACCCCTAAGTCAGCTGGTATCCGACTCAGACTCAGAGCTGGACAGCACAGA
  GCGGCTGGCCCTGGGAAGCACAGACACCTTGTCCAATGGACAGAAAGCAGATTTGGAGGCTGCACAGCGC
  CTAGCCAAGAGGCTATACCGACTCGATGGCTTCAGGAAGGCCGATGTGGCACGGCATCTGGGCAAGAACA
  ATGACTTCAGCAAATTGGTGGCTGGCGAGTATCTGAAGTTTTTTGTCTTCACGGGCATGACTCTGGACCA
  AGCTCTCAGGGTATTTCTAAAGGAGCTGGCCTTAATGGGTGAGACCCAGGAGCGGGAGCGTGTACTGGCC
  CACTTCTCCCAGCGATATTTCCAGTGTAATCCTGAAGCCCTGTCCTCAGAGGACGGTGCTCACACGCTGA
  CCTGTGCCCTCATGCTCCTCAACACAGATCTCCATGGCCATAACATTGGGAAGCGCATGACCTGTGGGGA
  CTTCATCGGAAACCTGGAGGGCCTCAATGATGGTGGCGACTTCCCCAGGGAGCTGCTCAAGGCCTTGTAC
  AGCTCCATCAAGAATGAAAAATTGCAATGGGCCATAGACGAGGAGGAGCTGCGACGGTCTCTGTCCGAGT
  TGGCCGATCCTAACCCCAAGGTCATCAAGCGGGTCAGCGGAGGCAGTGGCAGCAGTTCCAGCCCCTTCCT
  GGACCTGACTCCTGAGCCCGGGGCAGCTGTCTACAAGCACGGGGCCCTGGTGCGAAAGGTGCACGCAGAC
  CCTGACTGCAGGAAGACACCTCGTGGCAAGCGGGGCTGGAAGAGCTTCCACGGGATCCTCAAGGGCATGA
  TCCTCTACCTGCAGAAGGAGGAGTATCAGCCTGGGAAGGCTCTTTCCGAGGCAGAGCTGAAGAATGCTAT
  CAGCATCCACCACGCCCTGGCTACCCGCGCCAGCGATTATAGCAAGAGACCACACGTCTTCTACCTGCGC
  ACAGCTGACTGGCGGGTCTTCCTCTTCCAGGCTCCGAGCCTGGAGCAAATGCAGTCCTGGATCACTCGCA
  TCAATGTGGTGGCTGCCATGTTTTCTGCACCCCCCTTCCCAGCTGCTGTTAGCTCCCAGAAGAAATTCAG
  CCGCCCTCTGCTGCCCAGTGCTGCCACCCGCCTCTCCCAGGAGGAGCAGGTGAGGACCCATGAGGCCAAG
  CTGAAGGCCATGGCCAGCGAGCTGAGAGAGCACCGGGCTGCACATCTGGGAAAGAAGGCCCGAGGCAAGG
  AGGCGGATGAGCAGCGGCAGAAGGAGGCCTACCTGGAGTTTGAGAAATCCCGCTACGGCACCTATGCAGC
  ACTGCTTCGAGTCAAGATGAAGGCAGCCAGCGAGGAGCTGGACACCATAGAGGCAGCCCTGGCCCAGGCT
  GGGAGCACGGAGGATGGATGCCCTCCGCCTCACTCCAGTCCTTCCCTGCGGCCCAAGCCCACCAGCCAGC
  CCCGGGCTCAGCGTCCTGGCTCAGAAACCCGGGCTGGAGCAGGCAGTACAAGGCCAAAGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028627
• Insert Size: 3075 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028627.2, NP_082903.2
• RefSeq Size: 3950 bp
• RefSeq ORF: 3075 bp
• Locus ID: 73728
• UniProt ID: Q5DTT2
• Cytogenetics: 19 38.8 cM
• Gene Summary: Guanine nucleotide exchange factor for ARF6 (By similarity). Isoform 2 and isoform 3 induce cytoskeletal remodeling, but lead to distinct morphological changes in HeLa cells: isoform 2 induces cell elongation and formation of actin-rich protrusions, whereas isoform 3 promotes the formation of membrane ruffles and loss of stress fibers (PubMed:19494129).[UniProtKB/Swiss-Prot Function]